LOCUS       sequence-431694-        7708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting 
            Shank3 locus in the presence of Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Wang J, Lin J, Chen Y, Liu J, Zheng Q, Deng M, Wang R, Zhang Y, Feng
            S, Xu Z, Ye W, Hu Y, Duan J, Lin Y, Dai J, Chen Y, Li Y, Luo T, Chen
            Q, Lu Z
  TITLE     An ultra-compact promoter drives widespread neuronal expression in 
            mouse and monkey brains.
  JOURNAL   Cell Rep. 2023 Nov 28;42(11):113348. doi: 
            10.1016/j.celrep.2023.113348. Epub 2023 Oct 31.
  PUBMED    37910509
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-11-28"; volume: "42(11):113348. doi"; pages: " 
            10.1016/j.celrep.2023.113348. Epub 2023 Oct 31"
COMMENT     Sequence Label: pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3
FEATURES             Location/Qualifiers
     source          1..7708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     protein_bind    complement(265..298)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(349..382)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(386..433)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(434..4534)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(434..4534)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4559..4579)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4586..4612)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4615..4624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4626..4659
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    4666..4699
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4734..4754
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4866..4941
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     repeat_region   4971..5111
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4971..5100
                     /label=AAV2 ITR
     rep_origin      5186..5641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5273..5292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5483..5504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5658..5677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5777..5799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5837..5855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5923..6027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6028..6096,6097..6888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6246..6265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7059..7647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7548..7567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttacataact tcgtatagga tactttatac gaagttatgc
      301 agaatggtag ctggattgta gctgctatta gcaatatgaa acctcttaat aacttcgtat
      361 agcatacatt atacgaagtt atttactttt tcttttttgc ctggccggcc tttttcgtgg
      421 ccgccggcct tttgtcgcct cccagctgag acaggtcgat ccgtgtctcg tacagaccgg
      481 tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc
      541 ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg gtcagggtaa
      601 acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc ttgttgtagg
      661 cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag aactcgctga
      721 tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc tgtttctgct
      781 cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac aggaagttca
      841 catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca gaggccagca
      901 ttctcttccg gccgttttcc agctcgaaca gggagtactt aggcagcttg atgatcaggt
      961 cctttttcac ttctttgtag cccttggctt ccagaaagtc gatgggattc ttctcgaagc
     1021 tgcttctttc catgatggtg atccccagca gctctttcac actcttcagt ttcttggact
     1081 tgcccttttc cactttggcc accaccagca cagaataggc cacggtgggg ctgtcgaagc
     1141 cgccgtactt cttagggtcc cagtccttct ttctggcgat cagcttatcg ctgttcctct
     1201 tgggcaggat agactctttg ctgaagccgc ctgtctgcac ctcggtcttt ttcacgatat
     1261 tcacttgggg catgctcagc actttccgca cggtggcaaa atcccggccc ttatcccaca
     1321 cgatctcccc ggtttcgccg tttgtctcga tcagaggccg cttccggatc tcgccgttgg
     1381 ccagggtaat ctcggtcttg aaaaagttca tgatgttgct gtagaagaag tacttggcgg
     1441 tagccttgcc gatttcctgc tcgctcttgg cgatcatctt ccgcacgtcg tacaccttgt
     1501 agtcgccgta cacgaactcg ctttccagct tagggtactt tttgatcagg gcggttccca
     1561 cgacggcgtt caggtaggcg tcgtgggcgt ggtggtagtt gttgatctcg cgcactttgt
     1621 aaaactggaa atccttccgg aaatcggaca ccagcttgga cttcagggtg atcactttca
     1681 cttcccggat cagcttgtca ttctcgtcgt acttagtgtt catccgggag tccaggatct
     1741 gtgccacgtg ctttgtgatc tgccgggttt ccaccagctg tctcttgatg aagccggcct
     1801 tatccagttc gctcaggccg cctctctcgg ccttggtcag attgtcgaac tttctctggg
     1861 taatcagctt ggcgttcagc agctgccgcc agtagttctt catcttcttc acgacctctt
     1921 cggagggcac gttgtcgctc ttgccccggt tcttgtcgct tctggtcagc accttgttgt
     1981 cgatggagtc gtccttcaga aagctctgag gcacgatatg gtccacatcg tagtcggaca
     2041 gccggttgat gtccagttcc tggtccacgt acatatcccg cccattctgc aggtagtaca
     2101 ggtacagctt ctcgttctgc agctgggtgt tttccacggg gtgttctttc aggatctggc
     2161 tgcccagctc tttgatgccc tcttcgatcc gcttcattct ctcgcggctg ttcttctgtc
     2221 ccttctgggt ggtctggttc tctctggcca tttcgatcac gatgttctcg ggcttgtgcc
     2281 ggcccatcac tttcacgagc tcgtccacca ccttcactgt ctgcaggatg cccttcttaa
     2341 tggcggggct gccggccaga ttggcaatgt gctcgtgcag gctatcgccc tggccggaca
     2401 cctgggcttt ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc agctgcatga
     2461 agtttctgtt ggcgaagccg tcggacttca ggaaatccag gattgtcttg ccggactgct
     2521 tgtcccggat gccgttgatc agcttccggc tcagcctgcc ccagccggtg tatctccgcc
     2581 gcttcagctg cttcatcact ttgtcgtcga acaggtgggc ataggttttc agccgttcct
     2641 cgatcatctc tctgtcctca aacagtgtca gggtcagcac gatatcttcc agaatgtcct
     2701 cgttttcctc attgtccagg aagtccttgt ccttgataat tttcagcaga tcgtggtatg
     2761 tgcccaggga ggcgttgaac cgatcttcca cgccggagat ttccacggag tcgaagcact
     2821 cgattttctt gaagtagtcc tctttcagct gcttcacggt cactttccgg ttggtcttga
     2881 acagcaggtc cacgatggcc tttttctgct cgccgctcag gaaggcgggc tttctcattc
     2941 cctcggtcac gtatttcact ttggtcagct cgttatacac ggtgaagtac tcgtacagca
     3001 ggctgtgctt gggcagcacc ttctcgttgg gcaggttctt atcgaagttg gtcatccgct
     3061 cgatgaagct ctgggcggaa gcgcccttgt ccaccacttc ctcgaagttc cagggggtga
     3121 tggtttcctc gctctttctg gtcatccagg cgaatctgct gtttcccctg gccagagggc
     3181 ccacgtagta ggggatgcgg aaggtcagga tcttctcgat cttttcccgg ttgtccttca
     3241 ggaatgggta aaaatcttcc tgccgccgca gaatggcgtg cagctctccc aggtggatct
     3301 ggtgggggat gctgccgttg tcgaaggtcc gctgcttccg cagcaggtcc tctctgttca
     3361 gcttcacgag cagttcctcg gtgccgtcca tcttttccag gatgggcttg atgaacttgt
     3421 agaactcttc ctggctggct ccgccgtcaa tgtagccggc gtagccgttc ttgctctggt
     3481 cgaagaaaat ctctttgtac ttctcaggca gctgctgccg cacgagagct ttcagcaggg
     3541 tcaggtcctg gtggtgctcg tcgtatctct tgatcataga ggcgctcagg ggggccttgg
     3601 tgatctcggt gttcactctc aggatgtcgc tcagcaggat ggcgtcggac aggttcttgg
     3661 cggccagaaa caggtcggcg tactggtcgc cgatctgggc cagcaggttg tccaggtcgt
     3721 cgtcgtaggt gtccttgctc agctgcagtt tggcatcctc ggccaggtcg aagttgctct
     3781 tgaagttggg ggtcaggccc aggctcaggg caatcaggtt tccgaacagg ccattcttct
     3841 tctcgccggg cagctgggcg atcagatttt ccagccgtct gctcttgctc agtctggcag
     3901 acaggatggc cttggcgtcc acgccgctgg cgttgatggg gttttcctcg aacagctggt
     3961 tgtaggtctg caccagctgg atgaacagct tgtccacgtc gctgttgtcg gggttcaggt
     4021 cgccctcgat caggaagtgg ccccggaact tgatcatgtg ggccagggcc agatagatca
     4081 gccgcaggtc ggccttgtcg gtgctgtcca ccagtttctt tctcaggtgg tagatggtgg
     4141 ggtacttctc gtggtaggcc acctcgtcca cgatgttgcc gaagatgggg tgccgctcgt
     4201 gcttcttatc ctcttccacc aggaaggact cttccagtct gtggaagaag ctgtcgtcca
     4261 ccttggccat ctcgttgctg aagatctctt gcagatagca gatccggttc ttccgtctgg
     4321 tgtatcttct tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg ccgctgtcga
     4381 acagcagggc tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg cccagcacct
     4441 tgaatttctt gctgggcacc ttgtactcgt cggtgatcac ggcccagccc acagagttgg
     4501 tgccgatgtc caggccgatg ctgtacttct tgtcggctgc tgggactccg tggataccga
     4561 ccttccgctt cttctttggg gccatcgcgt aatctggaac atcgtatggg tagcccatgg
     4621 tggcaataac ttcgtataaa gtatcctata cgaagttatc aaagaataac ttcgtataat
     4681 gtatgctata cgaagttata aataaaatac gaaatgaaat aaaatacgaa atggagggcc
     4741 tatttcccat gattccttca tatttgcata tacgatagct taccgtaact tgaaagtatt
     4801 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggcagg agcccagcag
     4861 gctgggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     4921 aaagtggcac cgagtcggtg ctttttttga tgtgcggacc gagcggccgc aggaacccct
     4981 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     5041 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     5101 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5161 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     5281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5401 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     5521 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     5581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5641 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5701 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5761 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5821 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5881 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6241 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6541 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7681 gccttttgct ggccttttgc tcacatgt
//
