LOCUS       sequence-431700-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry tagged YIPF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH144_mCherry-YIPF4 WT
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..240
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    340..928
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(393..413)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(811..822)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1003..1236
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1355..1373)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1441..1545
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1546..1614,1615..2406)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1764..1783)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2577..3165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3066..3085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3319..3336
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3576..3597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3612..3617,3618..3635,3636..3642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3650..3666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3655..3677
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3674..3690
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3674..3690
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3740..3759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3951..4584
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4631..4756
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5253..5486
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5671..5715
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6012..6129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6181..6484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6485..6688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6639..6659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6685..6709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6791..7498
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6936..6954)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7204..7223
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    7523..7547
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8277..8301)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8314..8881
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8331..8883
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8475..8492)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8702..8721
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8895..9171,1..323)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8895..8914)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
       61 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
      121 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
      181 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
      241 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
      301 tgacccgcaa gcccggtgcc tgatgcatcg atggatccta atcaacctct ggattacaaa
      361 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      421 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      481 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      541 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      601 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      661 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
      721 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
      781 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
      841 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      901 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
      961 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     1021 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     1081 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1141 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1201 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     1261 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     1321 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     1381 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1441 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1501 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1561 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1621 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1681 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1741 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     1801 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1861 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1921 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1981 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2041 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2101 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2161 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2221 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2281 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2341 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2401 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2461 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2521 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2581 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2641 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2701 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2761 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2821 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2881 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2941 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3001 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3061 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3241 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3301 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3361 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
     3421 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3481 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
     3541 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3601 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3661 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
     3721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3781 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     3841 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     3901 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     3961 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     4021 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     4081 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     4141 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     4201 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     4261 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     4321 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     4381 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4441 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4501 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4561 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     4621 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4681 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4741 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4801 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     4861 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     4921 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     4981 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     5041 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     5101 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5161 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5221 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     5281 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     5341 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     5401 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     5461 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     5521 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     5581 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     5641 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     5701 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     5761 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     5821 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     5881 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     5941 tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca aatggcagta
     6001 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     6061 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     6121 caaaattttc gggtttatta cagggacagc agagatccag tttggactag tggagttccg
     6181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     6241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     6301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     6361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     6421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     6481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     6541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     6601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     6661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     6721 ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct ctcgaggttg
     6781 atctgctagc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6841 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     6901 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     6961 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     7021 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     7081 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     7141 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     7201 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     7261 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     7321 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     7381 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     7441 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     7501 atgcagcgct agtgcttatc aaacaagttt gtacaaaaaa gttggcatgc agcctccggg
     7561 cccgcccccg gcctatgccc ccactaacgg ggacttcacc tttgtctcct cagcagacgc
     7621 ggaagatctc agtggttcaa tagcatcccc agatgtcaaa ttaaatcttg gtggagattt
     7681 tatcaaagaa tctacagcta ctacatttct gagacaaaga ggttatggct ggcttctgga
     7741 agttgaagat gatgatcctg aagataacaa gccactcttg gaagaattgg acattgatct
     7801 aaaggatatt tactacaaaa tccgatgtgt tttgatgcca atgccatcac ttggttttaa
     7861 tagacaagtg gtgagagaca atcctgactt ttggggtcct ctggctgttg ttcttttctt
     7921 ttccatgata tcattatatg gacagtttag ggtggtctca tggattataa ccatttggat
     7981 atttggttca ctaacaattt tcttactggc cagagttctt ggtggagaag ttgcatatgg
     8041 ccaagtcctt ggagttatag gatattcatt acttcctctc attgtaatag cccctgtact
     8101 tttggtggtt ggatcatttg aagtggtgtc tacacttata aaactgtttg gtgtgttttg
     8161 ggctgcctac agtgctgctt cattgttagt gggtgaagaa ttcaagacca aaaagcctct
     8221 tctgatttat ccaatctttt tattatacat ttattttttg tcgttatata ctggtgtgcc
     8281 aactttcttg tacaaagtgg ttcgataacg aattccgccc ccccccccta acgttactgg
     8341 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     8401 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     8461 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8521 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     8581 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8641 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8701 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8761 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8821 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     8881 atatggccac aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     8941 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     9001 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     9061 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     9121 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc c
//
