LOCUS       sequence-431701-        9390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP tagged YIPF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH142_EGFP-YIPF3 WT
FEATURES             Location/Qualifiers
     source          1..9390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    302..423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(339..358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    449..1037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(502..522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(920..931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1112..1345
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1464..1482)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1550..1654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1655..1723,1724..2515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1873..1892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2686..3274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3175..3194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3428..3445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3685..3706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3721..3726,3727..3744,3745..3751)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3759..3775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3764..3786
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3783..3799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3783..3799
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4060..4693
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4740..4865
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5362..5595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5780..5824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6117..6234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6286..6589
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6590..6793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6744..6764
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6790..6814
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6873..6882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6879..6881,6882..6884,6885..7595)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6879..6881,6882..6884,6885..7595)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6924..6945)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7185..7204)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7532..7553
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    7611..7635
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8686..8710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8729..9228
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8764..8787)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9161..9180
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(9252..9390,1..260)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
       61 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
      121 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
      181 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
      241 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca ctcgacctcg
      301 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      361 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      421 ttatcatgtc tatgcatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
      481 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
      541 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
      601 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
      661 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
      721 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
      781 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
      841 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
      901 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
      961 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     1021 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     1081 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     1141 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     1201 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1261 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1321 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     1381 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     1441 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1501 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1621 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     1921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2341 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2521 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2581 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3301 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3361 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3421 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3481 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     3541 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3601 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
     3661 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     3721 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     3781 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
     3841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     3901 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     3961 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     4021 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     4081 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     4141 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     4201 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     4261 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     4321 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     4381 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     4441 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     4501 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4561 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4621 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4681 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     4741 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     4801 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     4861 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     4921 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     4981 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     5041 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     5101 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     5161 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     5221 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     5281 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     5341 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     5401 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     5461 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     5521 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     5581 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     5641 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     5701 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     5761 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     5821 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     5881 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     5941 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     6001 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     6061 agtgaacgga tctcgacggt atcgccgaat tcacaaatgg cagtattcat ccacaatttt
     6121 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     6181 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     6241 tattacaggg acagcagaga tccagtttgg actagtggag ttccgcgtta cataacttac
     6301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6421 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6481 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6541 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     6601 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6661 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6721 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     6781 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     6841 tgacctccat agaagacacc ggcggccgat ctgccaccat ggtgagcaag ggcgaggagc
     6901 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     6961 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     7021 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     7081 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     7141 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     7201 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     7261 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     7321 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     7381 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     7441 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     7501 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     7561 ccgggatcac tctcggcatg gacgagctgt acaaggctcg aggttatcaa acaagtttgt
     7621 acaaaaaagt tggcatggca actacagctg cgccggcggg cggcgcccga aatggagctg
     7681 gcccggaatg gggagggttc gaagaaaaca tccagggcgg aggctcagct gtgattgaca
     7741 tggagaacat ggatgatacc tcaggctcta gcttcgagga tatgggtgag ctgcatcagc
     7801 gcctgcgcga ggaagaagta gacgctgatg cagctgatgc agctgctgct gaagaggagg
     7861 atggagagtt cctgggcatg aagggcttta agggacagct gagccggcag gtggcagatc
     7921 agatgtggca ggctgggaaa agacaagcct ccagggcctt cagcttgtac gccaacatcg
     7981 acatcctcag accctacttt gatgtggagc ctgctcaggt gcgaagcagg ctcctggagt
     8041 ccatgatccc tatcaagatg gtcaacttcc cccagaaaat tgcaggtgaa ctctatggac
     8101 ctctcatgct ggtcttcact ctggttgcta tcctactcca tgggatgaag acgtctgaca
     8161 ctattatccg ggagggcacc ctgatgggca cagccattgg cacctgcttc ggctactggc
     8221 tgggagtctc atccttcatt tacttccttg cctacctgtg caacgcccag atcaccatgc
     8281 tgcagatgtt ggcactgctg ggctatggcc tctttgggca ttgcattgtc ctgttcatca
     8341 cctataatat ccacctccac gccctcttct acctcttctg gctgttggtg ggtggactgt
     8401 ccacactgcg catggtagca gtgttggtgt ctcggaccgt gggccccaca cagcggctgc
     8461 tcctctgtgg caccctggct gccctacaca tgctcttcct gctctatctg cattttgcct
     8521 accacaaagt ggtagagggg atcctggaca cactggaggg ccccaacatc ccgcccatcc
     8581 agagggtccc cagagacatc cctgccatgc tccctgctgc tcggcttccc accaccgtcc
     8641 tcaacgccac agccaaagct gttgcggtga ccctgcagtc acactgccca actttcttgt
     8701 acaaagtggt tcgataacga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     8761 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     8821 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     8881 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     8941 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     9001 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     9061 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     9121 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     9181 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     9241 ttaattaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     9301 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     9361 ctagcgacgg ccgcatcttc actggtgtca
//
