LOCUS       sequence-431702-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry tagged LIR mutant YIPF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH145_mCherry-YIPF4 LIR mut
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..80
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    377..494
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        546..849
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        850..1053
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1004..1024
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1050..1074
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1156..1863
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1301..1319)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1569..1588
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1888..1912
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2642..2666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2679..3246
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2696..3248
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2840..2857)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3067..3086
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3260..3859
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3260..3279)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3756..3776
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3876..4464
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3929..3949)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4347..4358)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4539..4772
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4891..4909)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4977..5081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5082..5150,5151..5942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5300..5319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6113..6701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6602..6621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6855..6872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7112..7133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7148..7153,7154..7171,7172..7178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7186..7202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7191..7213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7210..7226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7210..7226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7276..7295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7487..8120
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8167..8292
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8789..9022
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
       61 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
      121 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
      181 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
      241 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
      301 acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattaat tcacaaatgg
      361 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      421 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      481 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg actagtggag
      541 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      601 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      661 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      721 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      781 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      841 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      901 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      961 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1021 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1081 agacgccatc cacgctgttt tgacctccat agaagacacc ggcggccgca gatctctcga
     1141 ggttgatctg ctagcatggt gagcaagggc gaggaggata acatggccat catcaaggag
     1201 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     1261 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1321 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1381 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     1441 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     1501 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1561 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     1621 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     1681 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1741 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     1801 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1861 aaggcatgca gcgctagtgc ttatcaaaca agtttgtaca aaaaagttgg catgcagcct
     1921 ccgggcccgc ccccggccta tgcccccact aacggggacg ccacctttgc ctcctcagca
     1981 gacgcggaag atctcagtgg ttcaatagca tccccagatg tcaaattaaa tcttggtgga
     2041 gattttatca aagaatctac agctactaca tttctgagac aaagaggtta tggctggctt
     2101 ctggaagttg aagatgatga tcctgaagat aacaagccac tcttggaaga attggacatt
     2161 gatctaaagg atatttacta caaaatccga tgtgttttga tgccaatgcc atcacttggt
     2221 tttaatagac aagtggtgag agacaatcct gacttttggg gtcctctggc tgttgttctt
     2281 ttcttttcca tgatatcatt atatggacag tttagggtgg tctcatggat tataaccatt
     2341 tggatatttg gttcactaac aattttctta ctggccagag ttcttggtgg agaagttgca
     2401 tatggccaag tccttggagt tataggatat tcattacttc ctctcattgt aatagcccct
     2461 gtacttttgg tggttggatc atttgaagtg gtgtctacac ttataaaact gtttggtgtg
     2521 ttttgggctg cctacagtgc tgcttcattg ttagtgggtg aagaattcaa gaccaaaaag
     2581 cctcttctga tttatccaat ctttttatta tacatttatt ttttgtcgtt atatactggt
     2641 gtgccaactt tcttgtacaa agtggttcga taacgaattc cgcccccccc ccctaacgtt
     2701 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     2761 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     2821 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     2881 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     2941 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     3001 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     3061 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     3121 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     3181 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     3241 tgataatatg gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3301 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3361 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3421 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3481 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3541 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3601 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3661 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3721 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3781 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     3841 ccgcaagccc ggtgcctgat gcatcgatgg atcctaatca acctctggat tacaaaattt
     3901 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3961 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4021 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4081 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4141 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4201 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4261 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4321 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4381 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4441 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     4501 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     4561 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4621 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4681 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4741 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     4801 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
     4861 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4921 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5041 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5101 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5161 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5221 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5281 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     5341 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5401 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5461 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5521 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5581 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5641 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5701 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5761 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5821 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5881 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5941 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6001 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6061 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6241 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6781 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6841 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6901 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     6961 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7021 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     7081 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7141 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7201 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     7261 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     7321 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     7381 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     7441 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     7501 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7561 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7621 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7681 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7741 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7801 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7861 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7921 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7981 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8041 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8101 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8161 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     8221 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8281 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8341 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8401 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8461 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8521 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8581 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     8641 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8701 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     8761 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     8821 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8881 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8941 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     9001 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     9061 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     9121 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata c
//
