LOCUS       sequence-431704-        9390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP tagged LIR mutant YIPF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH143_EGFP-YIPF3 LIR mut
FEATURES             Location/Qualifiers
     source          1..9390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(30..74)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    259..492
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    989..1114
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1161..1794
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2055..2071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2055..2071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2068..2090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2079..2095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2103..2109,2110..2127,2128..2133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2148..2169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2409..2426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2580..3168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2660..2679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3339..4130,4131..4199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3962..3981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4200..4304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4372..4390
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4509..4742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4817..5405
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4923..4934
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5332..5352
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5431..5552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5496..5515
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5594..5992)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6016..6515)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6064..6083)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6457..6480
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    6534..6558
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7609..7633)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(7649..8359,8360..8362,8363..8365))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7649..8359,8360..8362,8363..8365))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7691..7712)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8040..8059
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8299..8320
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8362..8371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8430..8454)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8451..8654)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8480..8500)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8655..8958
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    9010..9127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
       61 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
      121 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
      181 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
      241 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
      301 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
      361 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
      421 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
      481 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg
      541 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc
      601 caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg
      661 cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt
      721 ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa
      781 gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt
      841 ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata
      901 ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc
      961 gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc
     1021 ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc
     1081 gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca
     1141 agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga
     1201 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     1261 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     1321 ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga
     1381 gggctcgcca ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa
     1441 gtcccttgta gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg
     1501 ccacgtgatg aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt
     1561 catccatccc atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct
     1621 cttctacctt atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat
     1681 atctgacccc tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt
     1741 agatccacag atcaaggata tcttgtcttc tttgggagtg aattagccct tccaactact
     1801 aagtttgtag tacatattta acaaatacaa tttctttaaa atgaaaataa ttcagaggaa
     1861 tcacaggttt agagtaaatg aaaccacagg taattggcag tggtaatagg gtatggggtg
     1921 ggaagtttgg gatgattttg gttagcttga gttatccagt tgatccagac atgataagat
     1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2041 aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2101 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2161 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccac
     2221 ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     2281 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgagc
     2341 ttgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     3961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4381 atcacgaggc cctttcgtct tcaagaatga tctagccctt tccttaatta acccgggcct
     4441 ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga
     4501 actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     4561 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     4621 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     4681 gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc
     4741 cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg
     4801 tcttaaagga tctcaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
     4861 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
     4921 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
     4981 caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg
     5041 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
     5101 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
     5161 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
     5221 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
     5281 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
     5341 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
     5401 tgattaggat ctatcgatgc atagacatga taagatacat tgatgagttt ggacaaacca
     5461 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5521 ttgtaaccat tataagctgc aataaacaag tttcgaggtc gagtgtcagt cctgctcctc
     5581 ggccacgaag tgcttagccc tcccacacat aaccagaggg cagcaattca cgaatcccaa
     5641 ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt gatcccagga tgcagatcga
     5701 gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca tttccgatcg
     5761 cgacgataca agtcaggttg ccagctgccg cagcagcagc agtgcccagc accacgagtt
     5821 ctgcacaagg tcccccagta aaatgatata cattgacacc agtgaagatg cggccgtcgc
     5881 tagagagagc tgcgctggcg acgctgtagt cttcagagat ggggatgctg ttgattgtag
     5941 ccgttgctct ttcaatgagg gtggattctt cttgagacaa aggcttggcc atggtttaat
     6001 taatgggctg caggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt
     6061 gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc
     6121 cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa
     6181 agcgaaggag caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc
     6241 agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct
     6301 gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg
     6361 cgcgaagggg ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt
     6421 gcgaggccag aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc
     6481 tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattcgttat cgaaccactt
     6541 tgtacaagaa agttgggcag tgtgactgca gggtcaccgc aacagctttg gctgtggcgt
     6601 tgaggacggt ggtgggaagc cgagcagcag ggagcatggc agggatgtct ctggggaccc
     6661 tctggatggg cgggatgttg gggccctcca gtgtgtccag gatcccctct accactttgt
     6721 ggtaggcaaa atgcagatag agcaggaaga gcatgtgtag ggcagccagg gtgccacaga
     6781 ggagcagccg ctgtgtgggg cccacggtcc gagacaccaa cactgctacc atgcgcagtg
     6841 tggacagtcc acccaccaac agccagaaga ggtagaagag ggcgtggagg tggatattat
     6901 aggtgatgaa caggacaatg caatgcccaa agaggccata gcccagcagt gccaacatct
     6961 gcagcatggt gatctgggcg ttgcacaggt aggcaaggaa gtaaatgaag gatgagactc
     7021 ccagccagta gccgaagcag gtgccaatgg ctgtgcccat cagggtgccc tcccggataa
     7081 tagtgtcaga cgtcttcatc ccatggagta ggatagcaac cagagtgaag accagcatga
     7141 gaggtccata gagttcacct gcaattttct gggggaagtt gaccatcttg atagggatca
     7201 tggactccag gagcctgctt cgcacctgag caggctccac atcaaagtag ggtctgagga
     7261 tgtcgatgtt ggcgtacaag ctgaaggccc tggaggcttg tcttttccca gcctgccaca
     7321 tctgatctgc cacctgccgg ctcagctgtc ccttaaagcc cttcatgccc aggaactctc
     7381 catcctcctc ttcagcagca gctgcatcag ctgcatcagc gtctacttct tcctcgcgca
     7441 ggcgctgatg cagctcaccc atatcctcgg cgctagagcc tgaggtatca tccatgttct
     7501 ccatgtcaat cacagctgag cctccgccct ggatgttttc ttcgaaccct ccccattccg
     7561 ggccagctcc atttcgggcg ccgcccgccg gcgccgctgt agttgccatg ccaacttttt
     7621 tgtacaaact tgtttgataa cctcgagcct tgtacagctc gtccatgccg agagtgatcc
     7681 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     7741 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     7801 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     7861 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     7921 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     7981 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     8041 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     8101 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     8161 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     8221 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     8281 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     8341 acagctcctc gcccttgctc accatggtgg cagatcggcc gccggtgtct tctatggagg
     8401 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
     8461 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     8521 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     8581 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     8641 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     8701 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     8761 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     8821 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     8881 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     8941 accgtaagtt atgtaacgcg gaactccact agtccaaact ggatctctgc tgtccctgta
     9001 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     9061 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     9121 tttaaaattg tggatgaata ctgccatttg tgaattcggc gataccgtcg agatccgttc
     9181 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
     9241 ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     9301 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     9361 actatcatta tgaataattt tatataccac
//
