LOCUS       sequence-431705-        3504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  endogenous mneon tag for YIPF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH163_pSmart-mNEON-YIPF4
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      712..741
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      763..1350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1251..1270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1362..1389)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1413..2204,2205..2273))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2036..2055
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2274..2376)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2377..2408)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(join(2951..3504,1..154))
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      3394..3403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggacttcag gtttaactcc tcataaccat catttggatt gccggtgccc tgacccacca
       61 tgtcaaagtc cacaccgttg atggagccaa agatgtgtaa ctcatgtgtc gctgggagag
      121 aggccatgtt atcctcctcg cccttgctca ccatttcacg tcgactccca accggccgcg
      181 gtagaagagg ctgtggttgt gcccagactc caagtcgacc acggcggcga cccgcacgtt
      241 caccttatgg tgcggcctac gagtctcgac gtgcaagctg cgagcgaggc cagaaacggg
      301 cacagcgggc cgctgagctg ccccgagact accccaccaa agtcttcttg gtggcgacca
      361 cccaagcacc acgacgccgg caccctgaca ggaccataac agtgacagcg gcgctgggat
      421 tggctctttg taatgtgctc tcccattggc tcccggagga agattctgat tgggtcttcc
      481 tgttgttgat tccggaagtt tacccaggac aggaagttca cttgtaattc gtggaaatct
      541 caggcctctt tttcctcctt gggtgctttt gttcctttaa gtgcgatctt tcctggcgtt
      601 ttgtttacct attacctccg tttttgttaa gtgggtggga acgaattcaa gcttgatatc
      661 attcaggacg agcctcagac tccagcgtaa ctggactgca atcaactcac tggctcacct
      721 tcacgggtgg gcctttcttc ggtagaaaat caaaggatct tcttgagatc ctttttttct
      781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      841 ggatcaagag ctaccaactc tttttccgag gtaactggct tcagcagagc gcagatacca
      901 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      961 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1021 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1081 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1141 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1201 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1261 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcatcg atttttgtga
     1321 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcagaaag gcccacccga
     1381 aggtgagcca ggtgattaca tttgggccct cattaccaat gcttaatcag tgaggcacct
     1441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1501 actacgatgc gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccgggagggc cgagcgcaga
     1621 agtgatcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1861 gccagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1921 cttactgtca tgccatccgt gagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcgt
     2101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact cattttagct
     2281 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     2341 ttatggtgaa agttggaacc tcttacgtgc cgatcaagtc aaaagcctcc ggtcggaggc
     2401 ttttgacttt ctgctatgga ggtcaggtat gatttataca taacagaaga aaacagccct
     2461 ccttcctttt tctcattctt tgagatctcc cttggccaat ctgtcctttt tctgagtaag
     2521 ggcgagtaat tccagaacta aaagcttagg attttgtggg tccaattgaa gagactaagc
     2581 cacccagaaa aaccagccac cagacggtat ggggaaaacc aagggctgac cgagtaactt
     2641 gatgctttaa aaagcagctc cctacacccc tccctccgtc cttcggggcc tctcggaagg
     2701 cccggcaggc gtggtgggct gcagtcacca gtcagcttcc tgcctgtccc cacccggcca
     2761 cgaccacctc ggcaccagaa agaccctacg gcgtcgggcc ctggcttcct ccgggtgata
     2821 gcccttccag gggctcagcc tcaccttccg cgtctgctga tgagacaaat gtaaaatcac
     2881 cgttagtagg agcatatgca ggaggtggac ctggaggttg catggagcct ccagacccac
     2941 ctgagcctcc cttgtacagc tcgtccatgc ccatcacatc ggtaaaggcc ttttgccact
     3001 ccttgaagtt gagctcggtc ttggagtgct tgagctccgt cttacggaac acgtacatcg
     3061 gctggttctt cagatagtta gccgccattg gcttggcaaa ggtgtaggtg gtccgcgcag
     3121 tgctccggta gcgcttgcca tttccagtgg tgtaactcca cttaaaggta ctgatgatgg
     3181 ttttgtcgtt ggggtaagtc ttcttcgacc tgcaccagtc cgcagcggtc agcgagttgg
     3241 tcatcacagg accgtcagca gggaaaccag tccccttcac ctgggcctct cctttgatgt
     3301 ggcttccctc gtaggtgtag cggtagttaa cagtaaggga ggcaccatct tcaaactgca
     3361 ttgtgcgatg gacttggtat ccggagccat ctaccatggc ggcctggaaa ggcgacatcc
     3421 cgtcagggta gggcaggtac tgatggaagc catacccgat atgagggacc agaatccagg
     3481 gggagaactg gaggtcaccc ttgg
//
