LOCUS       sequence-431712-        8508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably express the GolgiTag (GolgiIP handle) in mammalian cells 
            (mouse ORF). eGFP for cell sorting desired expression levels..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: pLV UBC msTMEM115-3C-3xHA-P2A-eGFP
FEATURES             Location/Qualifiers
     source          1..8508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(159..170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             363..596
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    668..789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(705..724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     759..778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      829..964
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(984..1003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(985..1003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1013..1030)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1013..1029)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1022..1044)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1171..1626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1258..1277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1468..1489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1652..1756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1757..1825,1826..2617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1975..1994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2788..3376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3277..3296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3530..3547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3664..3685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3700..3705,3706..3723,3724..3730)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3738..3754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3743..3765
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3762..3778
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3762..3778
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3797..3817
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3799..3817
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3845..4071
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4072..4252
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4299..4424
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4917..5150
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5335..5379
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5646..5762
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5765..6163
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             7284..7307
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7308..7334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7338..7364
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7368..7394
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7404..7460
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7461..7463,7464..7466,7467..8180)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7461..7463,7464..7466,7467..8177)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7506..7527)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7767..7786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8114..8135
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(8196..8508,1..276)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8249..8269)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
       61 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
      121 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
      181 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
      241 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
      301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
      361 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
      421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
      601 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
      661 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      781 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
      841 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
      901 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
      961 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     1021 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     1081 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1141 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     1201 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     1261 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     1321 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     1381 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     1441 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     1501 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     1561 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     1621 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1681 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1741 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1801 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1861 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1921 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1981 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2041 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2101 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2161 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatgggaga
     2221 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2281 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2341 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2401 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2461 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2521 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2581 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2641 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2701 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2761 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2821 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2881 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2941 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3001 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3061 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3121 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3241 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3301 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3361 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3421 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3481 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3541 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3601 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     3661 aattaatgtg agttagctca ctcattaggc accccaggct ttccacttta tgcttccggc
     3721 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3781 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     3841 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     3901 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     3961 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     4021 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     4081 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4141 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4201 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     4261 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     4321 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     4381 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     4441 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     4501 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     4561 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     4621 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     4681 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     4741 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     4801 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     4861 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     4921 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     4981 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     5041 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     5101 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     5161 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     5221 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     5281 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     5341 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     5401 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     5461 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     5521 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     5581 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     5641 ttaactttta aaagaaaagg ggggattggg gggtatagtg caggggaaag aatagtagac
     5701 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     5761 ttatggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
     5821 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
     5881 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
     5941 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
     6001 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
     6061 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
     6121 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggggagacg ccatccacgc
     6181 tgttttgacc tccatagaag acaccgacgc tagcggatcc accggtcgcc accatgcaaa
     6241 gggccttgcc aggagccaga caacacctag gtgctatctt ggcatccgcg tccgtcgttg
     6301 ttaaggcact ctgtgctgtt gtactcttcc tttatctcct ttccttcgct gttgacactg
     6361 gttgtctcgc cgtcactcct ggttacctct tcccacccaa tttctggatc tggacactcg
     6421 ctactcacgg actaatggag caacacgttt gggacgtcgc catctccttg gcaactgtcg
     6481 ttgttgcagg taggttgcta gagccccttt ggggagcttt ggagcttcta atattcttct
     6541 cagtcgtcaa tgtttctgta ggattgctcg gggccctcgc atatctcctc acatacatgg
     6601 catctttcaa tcttgtttac ctcttcacta tccgaatcca cggagccctc gggttccttg
     6661 gcggtgtact cgtagcattg aagcaaacaa tgggtgactg tgttgtcctt cgggttcctc
     6721 aagtccgagt ctcagtagtc cccatgctct tgctagcact attgctcctt ttgagacttg
     6781 ccactttgct acaatctcct gctcttgcct catacggttt cggactcctc tcctcttggg
     6841 tttacttgag gttctaccaa aggcactcta gagggagagg agacatggcc gaccacttcg
     6901 ctttcgccac attcttcccc gagatccttc aacccgtcgt tggacttttg gccaatttgg
     6961 ttcacggact acttgttaag gtaaagatct gtcaaaagac tgtaaagagg tacgacgttg
     7021 gggccccaag ttccatcaca atcagtttgc cagggacaga cccacaagac gcagagcgta
     7081 ggagacaact tgctcttaag gcattgaatg agaggcttaa gcgggtagag gaccaatctg
     7141 cttggccctc tatggacgac gacgaggaag aggccggggc aaagactgac tctccactcc
     7201 ctctcgagga agcctcaact ccacccggaa aggttacagt acccgagtcc agcttgatca
     7261 ctttggagac ggctcccttg ttgcttgagg tgctctttca gggaccctac ccgtatgatg
     7321 ttccggatta cgctggctat ccctacgacg tgcccgacta tgccgggtac ccctatgacg
     7381 tcccagacta cgcaggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg
     7441 tcgaagagaa tcctggaccg atggtgagca agggcgagga gctgttcacc ggggtggtgc
     7501 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     7561 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     7621 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     7681 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     7741 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     7801 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     7861 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     7921 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     7981 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     8041 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     8101 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     8161 tggacgagct gtacaagtaa agcggccgcg tcgacaatca acctctggat tacaaaattt
     8221 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8281 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8341 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8401 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8461 agctcctttc cgggactttc gctttccccc tccctattgc cacggcgg
//
