LOCUS       sequence-431713-       11727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to 
            sort for even expression levels..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 11727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1
FEATURES             Location/Qualifiers
     source          1..11727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1298..1886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1351..1371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1769..1780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1973..2206
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2278..2399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2315..2334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2369..2388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2439..2574
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2594..2613)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2595..2613)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2623..2640)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2623..2639)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2632..2654)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2781..3236
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2868..2887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3078..3099
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3262..3366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3367..3435,3436..4227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3585..3604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4398..4986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4887..4906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5140..5157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5274..5295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5310..5315,5316..5333,5334..5340)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5348..5364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5353..5375
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5372..5388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5372..5388
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5407..5427
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5409..5427
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5455..5681
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5682..5862
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5909..6034
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6527..6760
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6945..6989
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7256..7372
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7375..7773
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      7838..7847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7844..8539
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(7977..7995)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8245..8264
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8549..8605
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8609..8632
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8636..8659
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8663..8686
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    8702..8726
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8732..11727,1..1240)
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
ORIGIN
        1 gttaacacag gcagcccagg aacttgcagc agagaaagag aaaatatcag tattacaaaa
       61 caactatgaa aaaagtcagg aaactttcaa acagcttcaa tctgatttct atgggaggga
      121 atctgaactt ctagccacca ggcaagatct taagtctgta gaagagaagc tttctctagc
      181 acaggaggac ttgatttcaa acagaaatca aattggaaat caaaataaat tgattcaaga
      241 actgaagact gccaaggcta cattggagca ggattcagca aagaaagaac agcaattgca
      301 ggagcgatgt aaagcactac aagacattca gaaagaaaag tcactgaaag aaaaagaact
      361 ggtaaatgag aagtctaaat tggcagagat agaagaaatt aaatgtagac aagaaaaaga
      421 aatcactaaa ctaaacgaag aactcaagtc ccacaaacta gaaagcataa aggagataac
      481 aaatcttaaa gatgctaaac agcttctaat tcagcagaaa ttagaacttc aaggaaaagc
      541 ggactccctg aaggcagctg ttgaacagga gaagagaaat cagcagatac taaaagacca
      601 ggtgaaaaag gaagaagagg agctgaagaa agaatttatt gagaaagaag ctaagttgca
      661 ttccgaaata aaagaaaagg aagtaggaat gaagaagcat gaagaaaatg aggctaaact
      721 taccatgcag attacagcat taaatgaaaa cttaggcact gtgaagaagg agtggcaatc
      781 tagtcaacgg agagttagtg agcttgagaa acaaacggat gacttacggg gtgaaattgc
      841 agtattagaa gcaacggttc agaataatca agatgaaagg agagcactac tggaaagatg
      901 tcttaaagga gaaggtgaaa tagaaaagct tcaaaccaaa gtattagaat tgcaaagaaa
      961 gctggataat acaactgcag cagtgcagga gctgggcaga gaaaaccaat cacttcagat
     1021 caaacataca caagcgttga atagaaagtg ggccgaagac aatgaagtac aaaactgtat
     1081 ggcctgtggg aaaggctttt cagtaacagt gagacggcat cactgccgac agtgtggaaa
     1141 tatcttctgt gctgaatgtt cagccaaaaa tgccttaact ccttcctcca agaagcctgt
     1201 tcgtgtctgt gatgcatgtt tcaatgactt gcaaggataa agcggccgcg tcgacaatcg
     1261 tttcaatgac ttgcaaggat aaagcggccg cgtcgacaat caacctctgg attacaaaat
     1321 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     1381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     1441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     1501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     1561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     1621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     1681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     1741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     1801 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     1861 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt aagaccaatg
     1921 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1981 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     2041 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2101 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2161 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     2221 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     2281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2401 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2461 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2521 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     2581 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     2641 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2701 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2761 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3121 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     3241 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3301 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3361 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3421 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3481 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3541 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3601 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3661 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3721 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3781 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatgggaga tcatgtaact
     3841 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3901 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3961 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4021 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4081 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4141 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4201 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5221 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5281 agttagctca ctcattaggc accccaggct ttccacttta tgcttccggc tcgtatgttg
     5341 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     5401 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     5461 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     5521 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     5581 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     5641 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     5701 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5761 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5821 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     5881 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     5941 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     6001 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     6061 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     6121 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     6181 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     6241 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     6301 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     6361 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     6421 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6481 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6541 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     6601 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     6661 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     6721 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6781 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6841 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6901 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6961 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     7021 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7081 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     7141 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     7201 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     7261 aaagaaaagg ggggattggg gggtatagtg caggggaaag aatagtagac ataatagcaa
     7321 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatggcctc
     7381 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     7441 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     7501 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     7561 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     7621 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     7681 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     7741 tcttgtttgt ggatcgctgt gatcgtcact tggggagacg ccatccacgc tgttttgacc
     7801 tccatagaag acaccgacgc tagcggatcc accggtcgcc accatggtga gcaagggcga
     7861 ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca
     7921 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     7981 gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt
     8041 catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact actataagca
     8101 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt
     8161 gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg
     8221 cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc
     8281 gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct
     8341 gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa
     8401 gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa
     8461 cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg
     8521 catggacgag ctgtacaagg gatccggcgc aacaaacttc tctctgctga aacaagccgg
     8581 agatgtcgaa gagaatcctg gaccgatgga ctacaaggat gacgatgaca agggcgatta
     8641 caaggatgac gacgataagg gagattacaa ggatgacgac gataaggctc gaggttatca
     8701 aacaagtttg tacaaaaaag caggctccac catgttaagg aggattttac agaggactcc
     8761 tgggagagtt ggctctcaag gttctgattt agattcatca gcaactccta taaacacagt
     8821 ggacgtcaat aatgaaagct cttcagaggg tttcatatgt ccccagtgta tgaaatctct
     8881 tggatctgct gatgaacttt tcaaacatta tgaagctgtt catgatgctg gtaatgactc
     8941 aggtcatgga ggagagtcta atcttgcttt gaagcgagat gatgtaacac tgctcagaca
     9001 agaggtccaa gacctacagg cttcacttaa ggaagaaaaa tggtactcgg aagaattaaa
     9061 gaaggaatta gaaaaatatc aagggctgca gcagcaagag gccaaacctg atgggttggt
     9121 gactgattca tcagcagaac tacagtcttt ggaacagcaa ttagaagaag cccaaacaga
     9181 aaattttaat attaagcaaa tgaaagactt atttgaacag aaagcagccc aacttgctac
     9241 tgaaattgca gatataaagt caaagtatga tgaagaaagg agtcttcgag aagctgctga
     9301 acaaaaagtg acacgtctga cagaagaatt aaacaaagag gcaactgtaa ttcaagatct
     9361 gaagacggaa ctgcttcaga gacctggtat agaagatgtt gccgtgctaa agaaagaact
     9421 ggtccaagtt caaacactaa tggataacat gaccttggaa cgtgagcgag aatctgaaaa
     9481 actcaaagat gaatgcaaaa aattgcagtc acaatatgct agctcagagg ccacaataag
     9541 ccagctaagg agtgaacttg ccaaaggccc ccaggaagtt gctgtatatg tacaggaact
     9601 acaaaaactg aaaagttcag ttaatgaatt aacacaaaaa aatcagacct tgacagaaaa
     9661 cttgctgaaa aaagaacaag actatactaa gttagaggag aaacataatg aagaatctgt
     9721 gagtaaaaag aatattcagg caacccttca tcaaaaagac ctagattgtc aacagcttca
     9781 gtcaagattg tctgcatctg aaacctcact gcatagaata catgtagaac taagtgaaaa
     9841 aggagaagct actcaaaagc tcaaagaaga attatctgag gtagagacca agtaccagca
     9901 tctaaaggcg gagtttaagc agctacaaca acagagagaa gaaaaggagc agcatgggtt
     9961 acaactccaa agtgaaatta atcaattaca tagcaaactt ctggagacag agcgccaact
    10021 aggggaagct catggtaggc tgaaggaaca gagacagctt tcaagtgaaa agttgatgga
    10081 taaagaacaa caagtggctg atttacaact caaactttct cggttagaag agcagttgaa
    10141 ggaaaaagtt acaaattcta cagaattgca gcatcaatta gataaaacaa agcaacagca
    10201 tcaagaacaa caggctcttc agcaaagcac cacggcaaaa cttcgagaag ctcagaatga
    10261 tttggaacaa gttctacgtc aaattggcga taaggaccaa aagatccaga accttgaagc
    10321 tttattacag aagagtaaag aaaatatttc attactagaa aaagaaagag aagatcttta
    10381 tgcaaaaatt caggctggtg aaggagagac tgctgttctt aaccagttac aagaaaaaaa
    10441 ccatacacta caggagcaag taactcaact aacagagaag ctgaagaatc agtcagaaag
    10501 tcataaacaa gcccaggaga atttgcatga ccaggtacaa gagcagaagg cacatcttag
    10561 agctgcacaa gaccgtgtcc tttccctaga aactagtgtc aatgaattaa atagtcaatt
    10621 aaatgaaagc aaggagaagg tctcccagct tgacatacag attaaagcca aaaccgaact
    10681 attactatca gcagaagcag caaaaactgc tcaaagagct gatcttcaga atcatttgga
    10741 cacagctcaa aatgcattac aagataaaca gcaggagtta aataagatta ctactcagtt
    10801 ggatcaggtc actgcaaagt tacaagacaa gcaagaacat tgcagtcagc tggaaagtca
    10861 tcttaaagaa tataaagaga aatacctctc tttagaacag aaaaccgaag agctagaagg
    10921 tcaaattaag aaactagaag ctgatagtct tgaagttaaa gcaagcaagg agcaggcttt
    10981 gcaagatcta caacagcaaa gacagctgaa cacagattta gagctcagag ccacagaatt
    11041 gagtaaacaa cttgaaatgg agaaggaaat agtatccagt acaagattgg atctacagaa
    11101 aaaatctgaa gcccttgaaa gtatcaagca aaagcttacc aagcaagagg aagaaaaaaa
    11161 aatcctgaaa caagattttg aaactttaag tcaagaaaca aagattcagc atgaggaatt
    11221 gaataacaga attcaaacaa cagtaacaga actacaaaaa gtgaaaatgg agaaagaagc
    11281 tttaatgaca gagctttcta cagtaaagga caaactatca aaagtttctg attctttgaa
    11341 aaactctaaa agtgaatttg aaaaggagaa tcagaaagga aaagccgcta tattagactt
    11401 ggaaaaaact tgcaaagaat taaagcatca acttcaagtg cagatggaaa acacacttaa
    11461 ggaacagaag gaactgaaaa agtcacttga aaaagagaag gaggcttctc atcagttgaa
    11521 attggaactc aattcaatgc aggaacaact tatacaggcc cagaatactt taaaacaaaa
    11581 tgaaaaagaa gagcaacaac ttcaggggaa cataaatgag ctaaagcaat caagtgaaca
    11641 gaagaaaaaa caaattgaag cactccaagg agagcttaaa attgctgttt tacagaagac
    11701 agagcttgag aataaactac agcagca
//
