LOCUS       sequence-431714-       11266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express a ERphagy reporter in mammalian cells (untested).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11266)
  TITLE     Harper Lab plasmids
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
FEATURES             Location/Qualifiers
     source          1..11266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     199..219
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(333..366)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    422..1010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(475..495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(893..904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1013..1029)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1014..1030)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1219..1452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1524..1645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1561..1580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1615..1634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1685..1820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1840..1859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1841..1859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1882..2240)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1922..1943)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2134..2153
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2267..2371
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2373..2730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2373..2564
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2396..2416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2581..2716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2765..3559
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2765..3559
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2819..2838)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3429..3448
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3747..3766)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3791..3838
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4167..4755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4656..4675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4930..5233
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5235..5433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5388..5408
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5451..5631
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5678..5803
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6300..6533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6718..6762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7060..7177
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7269..8480
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8317..8336
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    8528..8552
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     8561..8614
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             8618..9502
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             join(9509..9511,9512..10222)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9551..9572)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9812..9831)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10159..10180
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(10247..10271)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10318..10407
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        10449..10948
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10484..10507)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10881..10900
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(10969..11266,1..302)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10969..10988)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
       61 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
      121 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
      181 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
      241 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
      301 gatgtacaag taggaattcg tcgagggacc taataacttc gtatagcata cattatacga
      361 agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga
      421 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
      481 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      541 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
      601 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
      661 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
      721 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
      781 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
      841 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
      901 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
      961 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     1021 tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     1081 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     1141 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     1201 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     1261 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     1321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     1381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     1441 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     1501 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     1561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1621 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     1681 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1741 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1801 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattag
     1861 catgtgagca aaaggccagc ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1921 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1981 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     2041 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     2101 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     2161 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     2221 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2281 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2341 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     2401 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2461 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     2521 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2581 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2641 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2701 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     2761 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     2821 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     2881 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     2941 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     3001 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     3061 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     3121 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     3181 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     3241 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     3301 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     3361 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     3421 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     3481 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     3541 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     3601 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     3661 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     3721 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     3781 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     3841 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     3901 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     3961 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     4021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4861 gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4921 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4981 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     5041 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5101 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     5161 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     5221 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     5281 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     5341 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     5401 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     5461 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5521 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5581 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     5641 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     5701 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     5761 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     5821 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     5881 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     5941 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6001 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6061 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6121 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     6181 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     6241 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     6301 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     6361 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     6421 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     6481 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     6541 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     6601 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     6661 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     6721 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     6781 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     6841 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     6901 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     6961 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     7021 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     7081 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     7141 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     7201 agatccagtt tggttagtac cgggcccgct ctagccctag atttaaccca acagaaggct
     7261 cgacgcgtgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     7321 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     7381 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     7441 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     7501 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     7561 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     7621 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     7681 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     7741 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     7801 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     7861 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     7921 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     7981 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     8041 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     8101 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     8161 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     8221 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     8281 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     8341 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     8401 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     8461 ttttggcttt tttgttagac gctagactgc cggatcgaat tgtcgacgaa ttcggatctg
     8521 gccaatcaca agtttgtaca aaaaagcagg ctccgccacc atgaagttat gcatattact
     8581 ggccgtcgtg gcctttgttg gcctctcgct cggatccgaa atcggtactg gctttccatt
     8641 cgacccccat tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg
     8701 cgatggcacc cctgtgctgt tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa
     8761 catcatcccg catgttgcac cgacccatcg ctgcattgct ccagacctga tcggtatggg
     8821 caaatccgac aaaccagacc tgggttattt cttcgacgac cacgtccgct tcatggatgc
     8881 cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc attcacgact ggggctccgc
     8941 tctgggtttc cactgggcca agcgcaatcc agagcgcgtc aaaggtattg catttatgga
     9001 gttcatccgc cctatcccga cctgggacga atggccagaa tttgcccgcg agaccttcca
     9061 ggccttccgc accaccgacg tcggccgcaa gctgatcatc gatcagaacg tttttatcga
     9121 gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg accattaccg
     9181 cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc
     9241 aatcgccggt gagccagcga acatcgtcgc gctggtcgaa gaatacatgg actggctgca
     9301 ccagtcccct gtcccgaagc tgctgttctg gggcacccca ggcgttctga tcccaccggc
     9361 cgaagccgct cgcctggcca aaagcctgcc taactgcaag gctgtggaca tcggcccagg
     9421 tctgaatctg ctgcaagaag acaacccgga cctgatcggc agcgagatcg cgcgctggct
     9481 gtctactctg gagatttccg gtctcgaggt gagcaagggc gaggagctgt tcaccggggt
     9541 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     9601 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     9661 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     9721 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     9781 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     9841 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     9901 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     9961 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
    10021 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
    10081 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaaactga gcaaagaccc
    10141 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
    10201 cggcatggac gagctgtaca agaattcaaa agatgagctc tgataaaccc agctttcttg
    10261 tacaaagtgg tgggtggagg cggttcaggt ggcggaggaa gcggaggcgg tggaagttac
    10321 ccctacgacg tgcccgacta cgccggctat ccgtatgatg tcccggacta tgcaggatcc
    10381 tatccatacg acgttccaga ttacgcttag ttaattaagg ccggccctcg aggttaacga
    10441 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
    10501 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
    10561 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
    10621 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
    10681 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
    10741 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
    10801 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
    10861 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
    10921 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac
    10981 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    11041 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    11101 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    11161 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    11221 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcg
//
