LOCUS       sequence-43172-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Nowotschin S, Eakin GS, Hadjantonakis AK
  TITLE     Dual transgene strategy for live visualization of chromatin and 
            plasma membrane dynamics in murine embryonic stem cells and 
            embryonic tissues.
  JOURNAL   Genesis. 2009 May;47(5):330-6.
  PUBMED    19358158
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2009-05"; volume: "47(5)"; pages: "330-6"
COMMENT     Sequence Label: pCAG:myr-mRFP1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     CDS             26..700
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     CDS             26..700
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(153..171)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     421..440
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     615..638
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(784..803)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 gggaagcagc aagagcaagc caaaggcctc ctccgaggac gtcatcaagg agttcatgcg
       61 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      121 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
      181 cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca aggcctacgt
      241 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
      301 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
      361 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
      421 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga tgtaccccga
      481 ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg gcggccacta
      541 cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc ccggcgccta
      601 caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
      661 gtacgagcgc gccgagggcc gccactccac cggcgcctaa gaattcactc ctcaggtgca
      721 ggctgcctat cagaangtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
      781 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
      841 ttctggctaa taaaggaaat t
//
