LOCUS       sequence-43173-e         558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Rhee JM, Pirity MK, Lackan CS, Long JZ, Kondoh G, Takeda J, 
            Hadjantonakis AK
  TITLE     In vivo imaging and differential localization of lipid-modified 
            GFP-variant fusions in embryonic stem cells and mice.
  JOURNAL   Genesis. 2006 Apr;44(4):202-18.
  PUBMED    16604528
REFERENCE   2  (bases 1 to 558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2006-04"; volume: "44(4)"; pages: "202-18"
COMMENT     Sequence Label: pCAG:myr-Venus
FEATURES             Location/Qualifiers
     source          1..558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..282)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     422..441
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggcagcctgc acctgaggag tgaattctta cttgtacagc tcgtccatgc cgagagtgat
       61 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      121 gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      181 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg
      241 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt
      301 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      361 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      421 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      481 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg
      541 caggccgtag cccagggt
//
