LOCUS       sequence-431735-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag-APEX2 tagged YIPF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH161_FLAG-APEX2-YIPF3 WT
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     410..430
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    530..1118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(583..603)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1001..1012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1193..1426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1545..1563)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1631..1735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1736..1804,1805..2596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1954..1973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2767..3355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3256..3275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3509..3526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3766..3787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3802..3807,3808..3825,3826..3832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3840..3856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3845..3867
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3864..3880
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3864..3880
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3930..3949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4141..4774
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4821..4946
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5443..5676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5861..5905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6202..6319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6371..6674
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6675..6878
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6829..6849
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6875..6899
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6987..7010
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7011..7757
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    7773..7797
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8848..8872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8885..9452
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8902..9454
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9046..9063)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9273..9292
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9466..9552,1..513)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9466..9485)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
       61 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
      121 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
      181 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
      241 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
      301 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
      361 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
      421 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
      481 acctggtgca tgacccgcaa gcccggtgcc tgatgcatcg atggatccta atcaacctct
      541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
      601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
      661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
      721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
      781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
      841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
      901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
      961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     1021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     1081 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg
     1141 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1201 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     1261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     1441 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc
     1501 gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct
     1561 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1621 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1681 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1741 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1801 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1861 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1921 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1981 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2041 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2101 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2161 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2221 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2281 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2341 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2401 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2461 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2521 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2581 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2641 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2701 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2881 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3481 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3541 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
     3601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3661 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
     3721 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3781 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3841 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
     3901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3961 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     4021 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     4081 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     4141 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     4201 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     4261 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     4321 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     4381 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     4441 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     4501 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     4561 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     4621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4741 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     4801 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     4861 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     4921 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     4981 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     5041 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     5101 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     5161 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     5221 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     5281 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     5341 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     5401 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     5461 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     5521 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     5581 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     5641 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     5701 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     5761 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     5821 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     5881 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     5941 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     6001 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     6061 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     6121 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     6181 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     6241 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     6301 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     6361 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     6421 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     6481 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     6541 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     6601 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     6661 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     6721 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     6781 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     6841 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     6901 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     6961 ctcgaggttg atctgccacc atgatggact acaaggatga cgacgataag ggaaagtctt
     7021 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag
     7081 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg
     7141 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg
     7201 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca
     7261 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg
     7321 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc
     7381 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt
     7441 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta
     7501 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta
     7561 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc
     7621 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg
     7681 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc
     7741 ttgggtttgc tgatgccgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatgg
     7801 caactacagc tgcgccggcg ggcggcgccc gaaatggagc tggcccggaa tggggagggt
     7861 tcgaagaaaa catccagggc ggaggctcag ctgtgattga catggagaac atggatgata
     7921 cctcaggctc tagcttcgag gatatgggtg agctgcatca gcgcctgcgc gaggaagaag
     7981 tagacgctga tgcagctgat gcagctgctg ctgaagagga ggatggagag ttcctgggca
     8041 tgaagggctt taagggacag ctgagccggc aggtggcaga tcagatgtgg caggctggga
     8101 aaagacaagc ctccagggcc ttcagcttgt acgccaacat cgacatcctc agaccctact
     8161 ttgatgtgga gcctgctcag gtgcgaagca ggctcctgga gtccatgatc cctatcaaga
     8221 tggtcaactt cccccagaaa attgcaggtg aactctatgg acctctcatg ctggtcttca
     8281 ctctggttgc tatcctactc catgggatga agacgtctga cactattatc cgggagggca
     8341 ccctgatggg cacagccatt ggcacctgct tcggctactg gctgggagtc tcatccttca
     8401 tttacttcct tgcctacctg tgcaacgccc agatcaccat gctgcagatg ttggcactgc
     8461 tgggctatgg cctctttggg cattgcattg tcctgttcat cacctataat atccacctcc
     8521 acgccctctt ctacctcttc tggctgttgg tgggtggact gtccacactg cgcatggtag
     8581 cagtgttggt gtctcggacc gtgggcccca cacagcggct gctcctctgt ggcaccctgg
     8641 ctgccctaca catgctcttc ctgctctatc tgcattttgc ctaccacaaa gtggtagagg
     8701 ggatcctgga cacactggag ggccccaaca tcccgcccat ccagagggtc cccagagaca
     8761 tccctgccat gctccctgct gctcggcttc ccaccaccgt cctcaacgcc acagccaaag
     8821 ctgttgcggt gaccctgcag tcacactgcc caactttctt gtacaaagtg gttcgataac
     8881 gaattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     8941 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     9001 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     9061 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     9121 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     9181 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     9241 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     9301 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     9361 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     9421 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgac cgagtacaag
     9481 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     9541 gcgttcgccg ac
//
