LOCUS       sequence-431736-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag-APEX2 tagged LIR mutant YIPF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH162_FLAG-APEX2-YIPF3 LIR mut
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(111..155)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    340..573
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1070..1195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1242..1875
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2067..2086)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2136..2152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2136..2152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2149..2171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2160..2176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2184..2190,2191..2208,2209..2214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2229..2250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2490..2507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2661..3249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2741..2760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3420..4211,4212..4280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4043..4062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4281..4385)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4453..4471
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4590..4823
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4898..5486
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5004..5015
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5413..5433
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5503..6102)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5586..5606)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6083..6102
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6114..6666
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6116..6683
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6276..6295)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6505..6522
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    6696..6720
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7771..7795)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7811..8557)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(8558..8581)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8669..8693)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8690..8893)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8719..8739)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8894..9197
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    9249..9366
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
       61 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
      121 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
      181 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
      241 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
      301 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
      361 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
      421 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
      481 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
      541 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
      601 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
      661 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
      721 agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
      781 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
      841 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
      901 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
      961 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     1021 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     1081 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     1141 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     1201 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     1261 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     1321 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     1381 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     1441 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     1501 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     1561 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     1621 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     1681 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     1741 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     1801 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
     1861 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
     1921 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
     1981 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
     2041 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2101 gaaaaaaatg ctttatttgt gaaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     2161 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2221 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2281 tcgggaaacc tgtcgtgcca cggggagctt tttgcaaaag cctaggcctc caaaaaagcc
     2341 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     2401 aaaaattagt cagccatgag cttgctgcat taatgaatcg gccaacgcgc ggggagaggc
     2461 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2521 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2581 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2821 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3061 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3181 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3301 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3361 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3661 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4021 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4261 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4321 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4381 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4441 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatg atctagccct
     4501 ttccttaatt aacccgggcc tctcactctc tgatattcat ttctttgcaa gttataaata
     4561 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     4621 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     4681 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     4741 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     4801 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     4861 agctgccttg taagtcattg gtcttaaagg atctcaggcg gggaggcggc ccaaagggag
     4921 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     4981 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     5041 cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac
     5101 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     5161 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     5221 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     5281 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     5341 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     5401 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
     5461 caaattttgt aatccagagg ttgattagga tccatcgatg catcaggcac cgggcttgcg
     5521 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
     5581 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
     5641 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
     5701 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
     5761 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
     5821 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
     5881 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
     5941 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
     6001 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
     6061 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggttgtgg ccatattatc
     6121 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
     6181 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat
     6241 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
     6301 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
     6361 tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct
     6421 gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt
     6481 ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa
     6541 tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata
     6601 tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag
     6661 taacgttagg gggggggggc ggaattcgtt atcgaaccac tttgtacaag aaagttgggc
     6721 agtgtgactg cagggtcacc gcaacagctt tggctgtggc gttgaggacg gtggtgggaa
     6781 gccgagcagc agggagcatg gcagggatgt ctctggggac cctctggatg ggcgggatgt
     6841 tggggccctc cagtgtgtcc aggatcccct ctaccacttt gtggtaggca aaatgcagat
     6901 agagcaggaa gagcatgtgt agggcagcca gggtgccaca gaggagcagc cgctgtgtgg
     6961 ggcccacggt ccgagacacc aacactgcta ccatgcgcag tgtggacagt ccacccacca
     7021 acagccagaa gaggtagaag agggcgtgga ggtggatatt ataggtgatg aacaggacaa
     7081 tgcaatgccc aaagaggcca tagcccagca gtgccaacat ctgcagcatg gtgatctggg
     7141 cgttgcacag gtaggcaagg aagtaaatga aggatgagac tcccagccag tagccgaagc
     7201 aggtgccaat ggctgtgccc atcagggtgc cctcccggat aatagtgtca gacgtcttca
     7261 tcccatggag taggatagca accagagtga agaccagcat gagaggtcca tagagttcac
     7321 ctgcaatttt ctgggggaag ttgaccatct tgatagggat catggactcc aggagcctgc
     7381 ttcgcacctg agcaggctcc acatcaaagt agggtctgag gatgtcgatg ttggcgtaca
     7441 agctgaaggc cctggaggct tgtcttttcc cagcctgcca catctgatct gccacctgcc
     7501 ggctcagctg tcccttaaag cccttcatgc ccaggaactc tccatcctcc tcttcagcag
     7561 cagctgcatc agctgcatca gcgtctactt cttcctcgcg caggcgctga tgcagctcac
     7621 ccatatcctc ggcgctagag cctgaggtat catccatgtt ctccatgtca atcacagctg
     7681 agcctccgcc ctggatgttt tcttcgaacc ctccccattc cgggccagct ccatttcggg
     7741 cgccgcccgc cggcgccgct gtagttgcca tgccaacttt tttgtacaaa cttgtttgat
     7801 aacctcgagc ggcatcagca aacccaagct cggaaagctt ttggtgagcc tcagcgtaat
     7861 cagcaaagaa ggcatcttcg tccgctgcat atttgtcaac gagagggcgg aatacagggt
     7921 cagacaaaag agccttgtca gaaggtagct gaaggagacc ttccttctca ccactcaaca
     7981 actccgtgaa gtatgagttg tcgaaaataa gaggattaga ggtccaggga ccctcaaatc
     8041 cagaacgctc cttgtgtgca gctccaatag tgtgaccccc agatagagca acgatatctt
     8101 ggtcagtaag ccccatagct ttgccaaaca catctctcaa atggtcagaa cccttagtgg
     8161 gatcgggcaa gcgaccctct ggtggtggct caggcttgtc ctctcttcca gggtggaatg
     8221 gaaccttagg tccacccgtg acctcaacgg caacaacgcc agccaactgg tagaaatcgg
     8281 cgtagctcaa aatagggaac tccgccttga gtggctccaa aagcctaaca gcgatgtcaa
     8341 gaccgttgtt agcgctgtga gccagttcgg cagggtgctt gatggttccg aagggtccac
     8401 cggtcttcgt gcccttgtca aaggttccag cagagtggaa tgccaaacgg agcattagag
     8461 gagcgcatct cttctcagcg atgaagcctc tgagcttctt cttcgccttc tcaacggcgt
     8521 cctggtaatc agcactcaca gttgggtaag actttccctt atcgtcgtca tccttgtagt
     8581 ccatcatggt ggcagatcaa cctcgagaga tctgcggccg ccggtgtctt ctatggaggt
     8641 caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta
     8701 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     8761 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     8821 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     8881 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     8941 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     9001 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     9061 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     9121 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     9181 ccgtaagtta tgtaacgcgg aactccacta gtccaaactg gatctctgct gtccctgtaa
     9241 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     9301 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     9361 ttaaaattgt ggatgaatac tgccatttgt gaattaattc ggcgataccg tcgagatccg
     9421 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     9481 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     9541 tgcctaactc ta
//
