LOCUS       sequence-431739-       12049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Deuschle K, Chaudhuri B, Okumoto S, Lager I, Lalonde S, Frommer WB
  TITLE     Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors 
            in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing 
            Mutants.
  JOURNAL   Plant Cell. 2006 Sep . 18(9):2314-25.
  PUBMED    16935985
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2006 Sep . 18(9):2314-25."
COMMENT     Sequence Label: pFLIPglu-2uDelta13
FEATURES             Location/Qualifiers
     source          1..12049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(357..359,360..362,363..875)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2409..2660
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    2893..2949
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2958..2974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2958..2974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2971..2993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2982..2998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3006..3012,3013..3030,3031..3036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3051..3072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3237..3261
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4572..5201
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5630..6703
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6769..6963
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7199..7221)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7307..7447
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7362..7381
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7462..7479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7633..8221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7713..7732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8467..9258)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    9779..9803
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        10175..10555
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    10597..10652
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             10654..11205
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      11232..11939
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga gggcccatgg
      361 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      421 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      481 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      541 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      601 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      661 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      721 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      781 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      841 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      901 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      961 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1021 tggagttcgt gaccgccgcc gggatcgctg atactcgcat tggtgtaaca atctataagt
     1081 acgacgataa ctttatgtct gtagtgcgca aggctattga gcaagatgcg aaagccgcgc
     1141 cagatgttca gctgctgatg aatgattctc agaatgacca gtccaagcag aacgatcaga
     1201 tcgacgtatt gctggcgaaa ggggtgaagg cactggcaat caacctggtt gacccggcag
     1261 ctgcgggtac ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt ttcttcaaca
     1321 aagaaccgtc tcgtaaggcg ctggatagct acgacaaagc ctactacgtt ggcactgact
     1381 ccaaagagtc cggcattatt caaggcgatt tgattgctaa acactgggcg gcgaatcagg
     1441 gttgggatct gaacaaagac ggtcagattc agttcgtact gctgaaaggt gaaccgggcg
     1501 ctccggatgc agaagcacgt accacttacg tgattaaaga attgaacgat aaaggcatca
     1561 aaactgaaca gttacagtta gataccgcaa tgtgggacac cgctcaggcg aaagataaga
     1621 tggacgcctg gctgtctggc ccgaacgcca acaaaatcga agtggttatc gccaacaacg
     1681 atgcgatggc aatgggcgcg gttgaagcgc tgaaagcaca caacaagtcc agcattccgg
     1741 tgtttggcgt cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt gcactggcgg
     1801 gcaccgtact gaacgatgct aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc
     1861 tggccgatgg taaaggtgcg gctgatggca ccaactggaa aatcgacaac aaagtggtcc
     1921 gcgtacctta tgttggcgta gataaagaca acctggctct cgagctgttc accggggtgg
     1981 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2041 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2101 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg
     2161 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2221 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2281 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2341 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2401 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2461 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2521 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca
     2581 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2641 gcatggacga gctgtacaag taatctagag tccgcaaaaa tcaccagtct ctctctacaa
     2701 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt
     2761 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     2821 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac
     2881 ctgcaggcat gcaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     2941 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc
     3181 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     3241 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     3301 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     3361 tcccctcggg atcaaagtac tttaaagtac tttgatccaa cccctccgct gctatagtgc
     3421 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
     3481 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
     3541 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
     3601 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
     3661 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
     3721 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
     3781 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
     3841 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
     3901 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
     3961 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
     4021 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
     4081 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
     4141 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
     4201 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
     4261 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
     4321 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
     4381 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     4441 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     4501 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     4561 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     4621 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     4681 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     4741 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     4801 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     4861 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     4921 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     4981 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     5041 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     5101 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     5161 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     5221 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     5281 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     5341 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     5401 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     5461 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     5521 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     5581 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     5641 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     5701 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     5761 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     5821 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     5881 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     5941 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     6001 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     6061 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     6121 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     6181 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     6241 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     6301 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     6361 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     6421 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     6481 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     6541 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     6601 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     6661 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     6721 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     6781 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     6841 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     6901 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     6961 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     7021 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     7081 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     7141 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7201 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7261 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7321 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7381 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7441 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8041 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8281 agggattttg gtcatgcatg atatatctcc caatttgtgt agggcttatt atgcacgctt
     8341 aaaaataata aaagcagact tgacctgata gtttggctgt gagcaattat gtgcttagtg
     8401 catctaacgc ttgagttaag ccgcgccgcg aagcggcgtc ggcttgaacg aatttctagc
     8461 tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
     8521 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
     8581 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
     8641 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
     8701 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
     8761 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
     8821 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
     8881 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
     8941 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
     9001 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
     9061 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
     9121 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
     9181 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
     9241 gatcaccgct tcccccatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac
     9301 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     9361 tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac
     9421 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     9481 ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgcccgaat
     9541 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     9601 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     9661 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     9721 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     9781 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     9841 cgtttttaat gtactgaatt aacgccgaat ttcgaggggc caacagagcc tggcgttccc
     9901 cttttgcatt gagaccgatg ttcgttccgg aaccttgcac gccccagagc ttctcaccgt
     9961 tcacgacaat ttccttctcg ttgaggtcgg tcgcgccatg tcggatgaaa taaaaacttt
    10021 tgataccagc gggggccttc gcagagccga ggtaggtctg agaaattggc attttcacgt
    10081 gtggaagata tgaatttttt tgagaaacta gataagatta atgaatatcg gtgttttggt
    10141 tttttcttgt ggccgtcttt gtttatattg agatttttca aatcagtgcg caagacgtga
    10201 cgtaagtatc cgagtcagtt tttatttttc tactaatttg gtcgtttatt tcggcgtgta
    10261 ggacatggca accgggcctg aatttcgcgg gtattctgtt tctattccaa ctttttcttg
    10321 atccgcagcc attaacgact tttgaataga tacgctgaca cgccaagcct cgctagtcaa
    10381 aagtgtacca aacaacgctt tacagcaaga acggaatgcg cgtgacgctc gcggtgacgc
    10441 catttcgcct tttcagaaat ggataaatag ccttgcttcc tattatatct tcccaaatta
    10501 ccaatacatt acactagcat ctgaatttca taaccaatct cgatacacca aatcgactct
    10561 agcgaattcc tcgagtataa gagctctatt tttacaacaa ttaccaacaa caacaaacaa
    10621 caaacaacat tacaattact atttacaatt accatgagcc cagaacgacg cccggccgac
    10681 atccgccgtg ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc
    10741 gagacaagca cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac
    10801 ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc
    10861 ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct acgactggac ggccgagtca
    10921 accgtgtacg tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac
    10981 ctgctgaagt ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc
    11041 aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg
    11101 gcggccggct tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc
    11161 ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga tttgatgacc cgggggatcg
    11221 atcctagagt cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt
    11281 tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc
    11341 ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat
    11401 tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac
    11461 aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac
    11521 aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta
    11581 aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac
    11641 gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg
    11701 cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg
    11761 caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat
    11821 gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga
    11881 cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg
    11941 acctgcaggc atgcaagctt gcatgcctgc aggtcaacat ggtggagcac gacactctcg
    12001 tctactccaa gaatatcaaa gatacagtct cagaagacca gagggctat
//
