LOCUS       sequence-431740-       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golgiphagy flux reporter GALNT2-keima.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH115_GALNT2-keima
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(411..455)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    640..873
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1370..1495
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1542..2175
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2367..2386)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2436..2452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2436..2452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2449..2471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2460..2476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2484..2490,2491..2508,2509..2514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2529..2550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2790..2807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2961..3549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3041..3060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3720..4511,4512..4580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4343..4362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4581..4685)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4753..4771
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             4890..5123
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5198..5786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5304..5315
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5713..5733
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5803..6402)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5886..5906)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6383..6402
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6414..6966
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6416..6983
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6576..6595)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6805..6822
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(6987..7649,7650..7652))
                     /codon_start=1
                     /product="humanized monomeric Keima-Red fluorescent 
                     protein"
                     /label=hmKeima-Red
                     /note="mammalian codon-optimized"
                     /translation="VSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKL
                     EGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG"
     protein_bind    7684..7708
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9422..9446)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9475..9494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9476..9494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9569..9593)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9590..9793)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9619..9639)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9794..10097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    join(10149..10152,1..114)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
       61 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
      121 atgaatactg ccatttgtga attaattcgg cgataccgtc gagatccgtt cactaatcga
      181 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
      241 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
      301 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
      361 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
      421 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
      481 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
      541 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
      601 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
      661 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
      721 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
      781 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
      841 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
      901 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
      961 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     1021 agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     1081 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     1141 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     1201 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     1261 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     1321 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     1381 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     1441 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     1501 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     1561 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     1621 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     1681 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     1741 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     1801 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     1861 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     1921 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     1981 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     2041 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     2101 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
     2161 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
     2221 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
     2281 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
     2341 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2401 gaaaaaaatg ctttatttgt gaaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     2461 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2521 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2581 tcgggaaacc tgtcgtgcca cggggagctt tttgcaaaag cctaggcctc caaaaaagcc
     2641 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     2701 aaaaattagt cagccatgag cttgctgcat taatgaatcg gccaacgcgc ggggagaggc
     2761 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3361 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3601 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3661 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3721 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3781 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3841 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3901 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3961 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4021 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4081 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4141 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4201 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4261 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4321 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4381 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4441 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4501 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4561 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4621 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4681 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4741 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatg atctagccct
     4801 ttccttaatt aacccgggcc tctcactctc tgatattcat ttctttgcaa gttataaata
     4861 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     4921 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     4981 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     5041 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     5101 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     5161 agctgccttg taagtcattg gtcttaaagg atctcaggcg gggaggcggc ccaaagggag
     5221 atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg
     5281 ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg
     5341 cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac
     5401 aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag
     5461 gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc
     5521 tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc
     5581 acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta
     5641 tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa
     5701 gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca
     5761 caaattttgt aatccagagg ttgattagga tccatcgatg catcaggcac cgggcttgcg
     5821 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
     5881 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
     5941 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
     6001 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
     6061 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
     6121 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
     6181 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
     6241 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
     6301 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
     6361 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggttgtgg ccatattatc
     6421 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
     6481 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat
     6541 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
     6601 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
     6661 tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct
     6721 gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt
     6781 ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa
     6841 tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata
     6901 tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag
     6961 taacgttagg gggggggggc ggaattctag cccagcaggg agtggcgggc gatggcgatc
     7021 tcgcactgct ccacgctggt gtagtcgcgg ttgtggctgg tcacgtccag cttgcggtcg
     7081 atctcgtggc ggccgggcat cctcacgggc ttcttggcct tgtaggtgct cttgaactcg
     7141 cacaggtagt ggccgccgcc ctccagcttc agggccatgt agtcgttgcc gatcagcatt
     7201 ccgtcgcggg cgaacaggcg ctcggtgctg ggctcccagc cctgggtctt cttctgcatc
     7261 acggggccgt tgggggggaa gttctcgccg ctgatcttca cgttgtagat gaagcagttg
     7321 ccctggatgc tgctgtcgtt gctcacggtg cacacggcgc cgtcctcgaa gttcatgctg
     7381 cgctcccagg tgtagccctc ggggaagctc tgcttgaagt agtcggggat gtcctcgggg
     7441 tacttggtga aggggatgct gccgtactgg agctgggggc tcaggatgtc ccaggcgaag
     7501 ggcagggggc cacccttggt cacggtcagc ttcacggtct gctcgccctc gtagggcttg
     7561 cccttgccgt cgccctccac ctcgaagtag tggccgttca cggtgccgct catgtacacc
     7621 ttgtaggtca tctgcttggc gatcacgctc acctcgagtt cgaaccgcgg gccctctaga
     7681 tcaaccactt tgtacaagaa agttgggtac tgctgcaggt tgagcgtgaa cttccactgc
     7741 tgcgaaaggg ccgggccaca cacctccacg cttaggcccc cgctcttggc cgtgcgactg
     7801 tccaggcaca ggttgctgcc cacgtgcctc agcttggagt tgccctcgat ctgttcccat
     7861 ttctgtctgc tgtcattttc tcggcagccc tgcagcttta taagagagcc cggtgcccgg
     7921 tccaccacag taaggcacaa atccatgtgc ttcactgact tctccttcgt caaggcccat
     7981 tcctggtttc ccccagcatt gtgacattca taaactccaa ccacaccatc agcaaagtgt
     8041 cccaaagtgt cgaggcagtt agttccctgc tgcaaggccc caaaagctat atcctgatgg
     8101 tctggaaccc ttaactctgg atagacattt tcaaggtacc atttgaaagg cttgcagctg
     8161 agtttcttcc taagctccaa tctgctctga atatttccat aaggaacgtt tctagcagaa
     8221 ggcactgctg cataatagaa atttttgtat tcatccatcc agacctctgc tgcccggcgg
     8281 gtgtttcggg caaagacagt gccactgcca cccgggaacg tgtaggggtg ctgcttccgg
     8341 aacacgtgtc ccacacggct gcacgggatg atctccaggc tgccaccaca ctgccacacg
     8401 cggaacgaga tctctaggtt ctctcctccc cacacatcca tcatcatgtc gtacttcccc
     8461 agttcttcaa aatagaactt atccatcaca aacagcccac cagcaatcat gggggttttt
     8521 ataggggcga ctgggttccc ctgccgggac cttctctgct caggcgtcat gtaatcccac
     8581 ttgaatacca agttccaatc aaaaccgccc ttcaagtcag cagatgcccc cacatactga
     8641 aagttgtcca tattaatgac atcgatgatg ggtgacacaa cccgagtcct gtcctccgcc
     8701 accctttcca ggaggggctc cagccagtgc tcattacact cgcagtgact gtccaggaag
     8761 gtcaggacct tggcttgggc agcatcggcc ccccgaaccc gtgagcgcat gaggccttct
     8821 cgtcgatcat ttctaagaac tcgcactttc tcaattttcc ccaagagagc cccgtcctca
     8881 ggatcattgc tgtagtcatc caccaagatg atttctttta tgagatgggg cgggcttttc
     8941 ttaagcacgc tgaccacggt cctgagtagg gccgacctgg cttcattgtg aaacgtgatc
     9001 accacgctgg tggccggcag atccacccgc cactgcttcc gctgacactg gtcatgccgg
     9061 gtgtcaggga tggctctgtc cattcgaagc ttatcactct ccacctggtt gaacttgttg
     9121 cgggcgtaag ggtcctgccc ggagcggacc atcgtccctc caacataagc ttcctggtta
     9181 aagtctggcc accgtacttt ccctggaggg agggtctcca tgctttgtgc tttctcttct
     9241 ccattgctgt gatgaaggtc tttcttttta atggggtcaa tttcattcca gtcctccttc
     9301 ctgccggcgc cgccgcccgc gcccccggcc agcgcagagc cgccccccga gtacatgtag
     9361 taggcgatgc ccagcaccca caggaaggcg aagcagagca gcatccgcga gcgccgccgc
     9421 atgccaactt ttttgtacaa acttgttgat agcttaacta gccagcttgg gtctccctat
     9481 agtgagtcgt attagatcaa cctcgagaga tctgcggccg ccggtgtctt ctatggaggt
     9541 caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta
     9601 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     9661 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     9721 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     9781 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     9841 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     9901 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     9961 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
    10021 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
    10081 ccgtaagtta tgtaacgcgg aactccacta gtccaaactg gatctctgct gtccctgtaa
    10141 taaacccgaa aa
//
