LOCUS       sequence-431743-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag-APEX2 tagged LIR mutant YIPF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Hickey KL, Swarup S, Smith IR, Paoli JC, Miguel Whelan E, Paulo JA, 
            Harper JW
  TITLE     Proteome census upon nutrient stress reveals Golgiphagy membrane 
            receptors.
  JOURNAL   Nature. 2023 Nov;623(7985):167-174. doi: 10.1038/s41586-023-06657-6.
            Epub 2023 Sep 27.
  PUBMED    37757899
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06657-6"; journalName: "Nature"; date: 
            "2023-11"; volume: "623"; issue: "7985"; pages: "167-174"
COMMENT     Sequence Label: pKH160_FLAG-APEX2-YIPF4 LIR mut
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     250..270
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    370..958
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(423..443)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(841..852)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1033..1266
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1385..1403)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1471..1575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1576..1644,1645..2436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1794..1813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2607..3195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3096..3115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3349..3366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3606..3627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3642..3647,3648..3665,3666..3672)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3680..3696
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3685..3707
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3704..3720
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3704..3720
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3770..3789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3981..4614
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4661..4786
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5283..5516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5701..5745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6042..6159
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6211..6514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6515..6718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6669..6689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6715..6739
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6827..6850
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6851..7597
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    7613..7637
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8367..8391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8404..8971
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8421..8973
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8565..8582)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8792..8811
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8985..9231,1..353)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8985..9004)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
       61 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
      121 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
      181 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
      241 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
      301 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgatgcatcg
      361 atggatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
      421 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
      481 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
      541 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
      601 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
      661 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
      721 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
      781 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
      841 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
      901 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
      961 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     1021 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     1081 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     1141 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     1201 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1261 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     1321 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     1381 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     1441 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1501 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     1561 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     1621 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     1681 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     1741 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     1801 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     1861 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1921 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1981 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2041 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     2101 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2161 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2221 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2281 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     2341 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     2401 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     2461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     2521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     2581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3361 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3421 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3481 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3541 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3601 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     3661 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     3721 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3781 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     3841 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     3901 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     3961 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     4021 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     4081 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     4141 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     4201 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     4261 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     4321 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     4381 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     4441 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4501 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4561 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     4621 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     4681 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     4741 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     4801 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     4861 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     4921 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     4981 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     5041 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     5101 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     5161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     5221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     5281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     5341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     5401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     5461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     5521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     5581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     5641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     5701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     5761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     5821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     5881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     5941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     6001 tatcgccgaa ttaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     6061 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     6121 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     6181 agagatccag tttggactag tggagttccg cgttacataa cttacggtaa atggcccgcc
     6241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     6301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     6361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     6421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     6481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     6541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     6601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     6661 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     6721 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     6781 acaccggcgg ccgcagatct ctcgaggttg atctgccacc atgatggact acaaggatga
     6841 cgacgataag ggaaagtctt acccaactgt gagtgctgat taccaggacg ccgttgagaa
     6901 ggcgaagaag aagctcagag gcttcatcgc tgagaagaga tgcgctcctc taatgctccg
     6961 tttggcattc cactctgctg gaacctttga caagggcacg aagaccggtg gacccttcgg
     7021 aaccatcaag caccctgccg aactggctca cagcgctaac aacggtcttg acatcgctgt
     7081 taggcttttg gagccactca aggcggagtt ccctattttg agctacgccg atttctacca
     7141 gttggctggc gttgttgccg ttgaggtcac gggtggacct aaggttccat tccaccctgg
     7201 aagagaggac aagcctgagc caccaccaga gggtcgcttg cccgatccca ctaagggttc
     7261 tgaccatttg agagatgtgt ttggcaaagc tatggggctt actgaccaag atatcgttgc
     7321 tctatctggg ggtcacacta ttggagctgc acacaaggag cgttctggat ttgagggtcc
     7381 ctggacctct aatcctctta ttttcgacaa ctcatacttc acggagttgt tgagtggtga
     7441 gaaggaaggt ctccttcagc taccttctga caaggctctt ttgtctgacc ctgtattccg
     7501 ccctctcgtt gacaaatatg cagcggacga agatgccttc tttgctgatt acgctgaggc
     7561 tcaccaaaag ctttccgagc ttgggtttgc tgatgccgct cgaggttatc aaacaagttt
     7621 gtacaaaaaa gttggcatgc agcctccggg cccgcccccg gcctatgccc ccactaacgg
     7681 ggacgccacc tttgcctcct cagcagacgc ggaagatctc agtggttcaa tagcatcccc
     7741 agatgtcaaa ttaaatcttg gtggagattt tatcaaagaa tctacagcta ctacatttct
     7801 gagacaaaga ggttatggct ggcttctgga agttgaagat gatgatcctg aagataacaa
     7861 gccactcttg gaagaattgg acattgatct aaaggatatt tactacaaaa tccgatgtgt
     7921 tttgatgcca atgccatcac ttggttttaa tagacaagtg gtgagagaca atcctgactt
     7981 ttggggtcct ctggctgttg ttcttttctt ttccatgata tcattatatg gacagtttag
     8041 ggtggtctca tggattataa ccatttggat atttggttca ctaacaattt tcttactggc
     8101 cagagttctt ggtggagaag ttgcatatgg ccaagtcctt ggagttatag gatattcatt
     8161 acttcctctc attgtaatag cccctgtact tttggtggtt ggatcatttg aagtggtgtc
     8221 tacacttata aaactgtttg gtgtgttttg ggctgcctac agtgctgctt cattgttagt
     8281 gggtgaagaa ttcaagacca aaaagcctct tctgatttat ccaatctttt tattatacat
     8341 ttattttttg tcgttatata ctggtgtgcc aactttcttg tacaaagtgg ttcgataacg
     8401 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     8461 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     8521 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     8581 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     8641 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     8701 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     8761 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     8821 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     8881 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     8941 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgacc gagtacaagc
     9001 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     9061 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     9121 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     9181 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc g
//
