LOCUS       sequence-431744-       10241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generate lentiviruses encoding for OCT4, KLF4 and c-MYC 
            (polycistronic vector); used in conjunction with SOX vectors for 
            pluripotency reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Ho SY, Hu H, Ho DHH, Renom APS, Yeung SW, Boerner F, Weng M, 
            Hutchins AP, Jauch R
  TITLE     An acidic residue within the OCT4 dimerization interface of SOX17 is
            necessary and sufficient to overcome its pluripotency-inducing 
            activity.
  JOURNAL   Stem Cell Reports. 2025 Mar 11;20(3):102398. doi: 
            10.1016/j.stemcr.2025.102398. Epub 2025 Feb 6.
  PUBMED    39919754
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports."; date: "2025-03-11"; volume: "20(3):102398. doi"; pages: "
            10.1016/j.stemcr.2025.102398. Epub 2025 Feb 6"
COMMENT     Sequence Label: pHAGE2-TetO-OCT4-KLF4-cMYC
FEATURES             Location/Qualifiers
     source          1..10241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3780..3833
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5253..5309
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5310..6629
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    6649..7237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6702..6722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7120..7131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7367..7400)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7419..7599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7646..7664)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7732..7836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7837..7905,7906..8697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8055..8074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8868..9456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9610..9627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9867..9888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9903..9908,9909..9926,9927..9933)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9941..9957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9946..9968
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9965..9981
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9965..9981
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10031..10050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc caccaccggt atggcgggac
     2701 acctggcttc ggatttcgcc ttctcgcccc ctccaggtgg tggaggtgat gggccagggg
     2761 ggccggagcc gggctgggtt gatcctcgga cctggctaag cttccaaggc cctcctggag
     2821 ggccaggaat cgggccgggg gttgggccag gctctgaggt gtgggggatt cccccatgcc
     2881 ccccgccgta tgagttctgt ggggggatgg cgtactgtgg gccccaggtt ggagtggggc
     2941 tagtgcccca aggcggcttg gagacctctc agcctgaggg tgaagcagga gtcggggtgg
     3001 agagcaactc cgatggggcc tccccggagc cctgcaccgt cacccctggt gccgtgaagc
     3061 tggagaagga gaagctggag caaaacccgg aggagtccca ggacatcaaa gctctgcaga
     3121 aagaactcga gcaatttgcc aagctcctga agcagaagag gatcaccctg ggatatacac
     3181 aggccgatgt ggggctcacc ctgggggttc tatttgggaa ggtattcagc caaacgacca
     3241 tctgccgctt tgaggctctg cagcttagct tcaagaacat gtgtaagctg cggcccttgc
     3301 tgcagaagtg ggtggaggaa gctgacaaca atgaaaatct tcaggagata tgcaaagcag
     3361 aaaccctcgt gcaggcccga aagagaaagc gaaccagtat cgagaaccga gtgagaggca
     3421 acctggagaa tttgttcctg cagtgcccga aacccacact gcagcagatc agccacatcg
     3481 cccagcagct tgggctcgag aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga
     3541 agggcaagcg atcaagcagc gactatgcac aacgagagga ttttgaggct gctgggtctc
     3601 ctttctcagg gggaccagtg tcctttcctc tggccccagg gccccatttt ggtaccccag
     3661 gctatgggag ccctcacttc actgcactgt actcctcggt ccctttccct gagggggaag
     3721 cctttccccc tgtctctgtc accactctgg gctctcccat gcattcaaac ggatctggag
     3781 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cccatggctg
     3841 tcagcgacgc gctgctccca tctttctcca cgttcgcgtc tggcccggcg ggaagggaga
     3901 agacactgcg tcaagcaggt gccccgaata accgctggcg ggaggagctc tcccacatga
     3961 agcgacttcc cccagtgctt cccggccgcc cctatgacct ggcggcggcg accgtggcca
     4021 cagacctgga gagcggcgga gccggtgcgg cttgcggcgg tagcaacctg gcgcccctac
     4081 ctcggagaga gaccgaggag ttcaacgatc tcctggacct ggactttatt ctctccaatt
     4141 cgctgaccca tcctccggag tcagtggccg ccaccgtgtc ctcgtcagcg tcagcctcct
     4201 cttcgtcgtc gccgtcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcacct
     4261 atccgatccg ggccgggaac gacccgggcg tggcgccggg cggcacgggc ggaggcctcc
     4321 tctatggcag ggagtccgct ccccctccga cggctccctt caacctggcg gacatcaacg
     4381 acgtgagccc ctcgggcggc ttcgtggccg agctcctgcg gccagaattg gacccggtgt
     4441 acattccgcc gcagcagccg cagccgccag gtggcgggct gatgggcaag ttcgtgctga
     4501 aggcgtcgct gagcgcccct ggcagcgagt acggcagccc gtcggtcatc agcgtcagca
     4561 aaggcagccc tgacggcagc cacccggtgg tggtggcgcc ctacaacggc gggccgccgc
     4621 gcacgtgccc caagatcaag caggaggcgg tctcttcgtg cacccacttg ggcgctggac
     4681 cccctctcag caatggccac cggccggctg cacacgactt ccccctgggg cggcagctcc
     4741 ccagcaggac taccccgacc ctgggtcttg aggaagtgct gagcagcagg gactgtcacc
     4801 ctgccctgcc gcttcctccc ggcttccatc cccacccggg gcccaattac ccatccttcc
     4861 tgcccgatca gatgcagccg caagtcccgc cgctccatta ccaagagctc atgccacccg
     4921 gttcctgcat gccagaggag cccaagccaa agaggggaag acgatcgtgg ccccggaaaa
     4981 ggaccgccac ccacacttgt gattacgcgg gctgcggcaa aacctacaca aagagttccc
     5041 atctcaaggc acacctgcga acccacacag gtgagaaacc ttaccactgt gactgggacg
     5101 gctgtggatg gaaattcgcc cgctcagatg aactgaccag gcactaccgt aaacacacgg
     5161 ggcaccgccc gttccagtgc caaaaatgcg accgagcatt ttccaggtcg gaccacctcg
     5221 ccttacacat gaagaggcat tttggatctg gagcaacaaa cttctcacta ctcaaacaag
     5281 caggtgacgt ggaggagaat cccggcccta tgcccctcaa cgttagcttc accaacagga
     5341 actatgacct cgactacgac tcggtgcagc cgtatttcta ctgcgacgag gaggagaact
     5401 tctaccagca gcagcagcag agcgagctgc agcccccggc gcccagcgag gatatctgga
     5461 agaaattcga gctgctgccc accccgcccc tgtcccctag ccgccgctcc gggctctgct
     5521 cgccctccta cgttgcggtc acacccttct cccttcgggg agacaacgac ggcggtggcg
     5581 ggagcttctc cacggccgac cagctggaga tggtgaccga gctgctggga ggagacatgg
     5641 tgaaccagag tttcatctgc gacccggacg acgagacctt catcaaaaac atcatcatcc
     5701 aggactgtat gtggagcggc ttctcggccg ccgccaagct cgtctcagag aagctggcct
     5761 cctaccaggc tgcgcgcaaa gacagcggca gcccgaaccc cgcccgcggc cacagcgtct
     5821 gctccacctc cagcttgtac ctgcaggatc tgagcgccgc cgcctcagag tgcatcgacc
     5881 cctcggtggt cttcccctac cctctcaacg acagcagctc gcccaagtcc tgcgcctcgc
     5941 aagactccag cgccttctct ccgtcctcgg attctctgct ctcctcgacg gagtcctccc
     6001 cgcagggcag ccccgagccc ctggtgctcc atgaggagac accgcccacc accagcagcg
     6061 actctgagga ggaacaagaa gatgaggaag aaatcgatgt tgtttctgtg gaaaagaggc
     6121 aggctcctgg caaaaggtca gagtctggat caccttctgc tggaggccac agcaaacctc
     6181 ctcacagccc actggtcctc aagaggtgcc acgtctccac acatcagcac aactacgcag
     6241 cgcctccctc cactcggaag gactatcctg ctgccaagag ggtcaagttg gacagtgtca
     6301 gagtcctgag acagatcagc aacaaccgaa aatgcaccag ccccaggtcc tcggacaccg
     6361 aggagaatgt caagaggcga acacacaacg tcttggagcg ccagaggagg aacgagctaa
     6421 aacggagctt ttttgccctg cgtgaccaga tcccggagtt ggaaaacaat gaaaaggccc
     6481 ccaaggtagt tatccttaaa aaagccacag catacatcct gtccgtccaa gcagaggagc
     6541 aaaagctcat ttctgaagag gacttgttgc ggaaacgacg agaacagttg aaacacaaac
     6601 ttgaacagct acggaactct tgtgcgtgag tcgacatcga tagatcctaa tcaacctctg
     6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7201 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     7261 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7321 aattcactcc caacgaagac aagatcacct gcaggacagg cgcgccataa cttcgtatag
     7381 catacattat acgaagttat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7441 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7501 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7561 ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccgggcgatt aaggaaaggg
     7621 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7681 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7801 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7861 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7921 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7981 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8041 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     8101 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8161 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8221 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8281 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8341 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8401 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8461 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8521 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8581 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8641 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8701 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8761 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8821 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8881 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8941 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9001 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9061 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9121 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9181 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9241 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9301 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9361 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9421 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9481 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9541 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9601 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     9661 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
     9721 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     9781 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
     9841 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9901 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9961 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    10021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    10081 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    10141 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    10201 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
