LOCUS       sequence-431746-        8511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably express the GolgiTag (GolgiIP handle) in mammalian cells 
            (human ORF). eGFP for cell sorting desired expression levels..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
FEATURES             Location/Qualifiers
     source          1..8511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    248..373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             420..600
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(601..827)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(855..875)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(855..873)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(894..910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(894..910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(907..929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    918..934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(942..948,949..966,967..972))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    987..1008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1125..1142)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1296..1884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1376..1395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2055..2846,2847..2915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2678..2697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2916..3020)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3046..3501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3183..3204)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3395..3414
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3628..3650
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3642..3659
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3643..3659
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3669..3688
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3669..3687
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3708..3843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3883..4004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3894..3913)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3948..3967
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4076..4309
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4396..4984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4502..4513
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4911..4931
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5000..5713,5714..5716,5717..5719))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5003..5713,5714..5716,5717..5719))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5045..5066)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5394..5413
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5653..5674
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5720..5776)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5786..5812)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5816..5842)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5846..5872)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5873..5896)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        complement(7020..7418)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     misc_feature    7421..7537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7804..7848)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8033..8266
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa
       61 gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag
      121 gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt
      181 ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct
      241 taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg
      301 gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc
      361 tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact
      421 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
      481 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
      541 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
      601 gtttatgtat cgagctaggc acttaaatac aatatctctg caatgcggca attcagtggt
      661 tcgtccaatc catgtcagac ccgtctgttg ccttcctaat aaggcacgat cgtaccacct
      721 tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat gttgctaact
      781 catcgttacc atgttgcaag actacaagag tattgcataa gactacatta agcttgcagc
      841 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      901 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      961 ataaagtgga aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1021 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1081 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2341 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atctcccatg
     2461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2641 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3001 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     3061 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3121 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3181 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3241 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3301 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3361 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3421 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3481 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     3541 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3601 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3661 agcgcgcgta atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa
     3721 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     3781 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     3841 gatgggcgga gttaggggcg ggatagctag agccagacat gataagatac attgatgagt
     3901 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3961 ctattgcttt atttgtaacc attataagct gcaataaaca agttcctctc actctctgat
     4021 attcatttct ttgcaagtta taaatactga ataataagat gacatgaact actactgcta
     4081 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     4141 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     4201 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     4261 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     4321 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
     4381 gagctcgaat tccaggcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa
     4441 ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg
     4501 gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac
     4561 aggcgagcag ccatggaaag gacgtcagct tccccgacaa caccacggaa ttgtcagtgc
     4621 ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg
     4681 caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc
     4741 aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg
     4801 gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag
     4861 ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa
     4921 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
     4981 gattgtcgac gcggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
     5041 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     5101 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     5161 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     5221 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     5281 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     5341 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     5401 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     5461 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     5521 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     5581 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     5641 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     5701 cctcgccctt gctcaccatc ggtccaggat tctcttcgac atctccggct tgtttcagca
     5761 gagagaagtt tgttgcgccg gatcctgcgt agtctgggac gtcatagggg tacccggcat
     5821 agtcgggcac gtcgtaggga tagccagcgt aatccggaac atcatacggg tagggtccct
     5881 gaaagagcac ctcaagcagc gtcgggggag ctgcctcgaa ggtgattaga ctggattctg
     5941 gggcagcccc cttccctggg ggtgtgggag ctttgtctga gggcaggggg ctgtccacct
     6001 tggccccaga ctcctcttca tcatcatcca tgctgggcca gatggactgg tcttccactc
     6061 tcttcagccg ctcattgagt gccttcaggg ccagttgcct tctccgctcg gcgtcttgag
     6121 ggtctgtgcc tggcaggctg atggtgatgg aggatggggc acccacatcg tagcgcttca
     6181 ccgtcttctg gcatatcttt accttcacca ggaggctgtg caccaagttc gccagcaaac
     6241 ccaccacagg ctgcaggatc tcagggaaga aagtggcgaa agcaaagtgg tcagccatgt
     6301 cccctcggcc ccggctatgg cgctggtaga agcgaagata tacccaactg gagagcagcc
     6361 cgaagccata ggaagccagc gccgggctct ggagcagcgt ggcgagccgc agcaggagca
     6421 gcagcgccag cagcagcatg ggcatcacac tgacgcgcac ctggggcact cgcaggacca
     6481 cacagtcccc catggtttgc ttgagtgcca ccaggacgcc acctaggaag cccaaggcgc
     6541 cgtggatacg gacagtgaac aggtagacca ggttgaagga agccatgtag gtgaggaggt
     6601 aggcgaaggc ccccagcagc cctacagaca cattcaccac tgagaagaag atgagcagct
     6661 ccaaggcccc ccagaggggc tccagcaaac gcccggccac caccaccgtt gtcaggctga
     6721 tggccacgtc ccacacatgc tgctccatca gcccatgggt ggccagggtc cagatccaga
     6781 agttgggagg aaagaggtag cccggggtga ccgccaggca gcctgtgtcc acggcgaagg
     6841 agagcaggta gaggaatagt accgccgcac acagagcctt caccaccacg ctggcgctgg
     6901 ccagaatggc ccccaagtgc tggcgggcgc ctggcagggc acgttgcatg gtggcgaccg
     6961 gtggatccgc tagcgtcggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctccc
     7021 caagtgacga tcacagcgat ccacaaacaa gaaccgcgac ccaaatcccg gctgcgacgg
     7081 aactagctgt gccacacccg gcgcgtcctt atataatcat cggcgttcac cgccccacgg
     7141 agatccctcc gcagaatcgc cgagaaggga ctacttttcc tcgcctgttc cgctctctgg
     7201 aaagaaaacc agtgccctag agtcacccaa gtcccgtcct aaaatgtcct tctgctgata
     7261 ctggggttct aaggccgagt cttatgagca gcgggccgct gtcctgagcg tccgggcgga
     7321 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
     7381 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccat aaaattttga attttgtaat
     7441 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     7501 tgcactatac cccccaatcc ccccttttct tttaaaagtt aaccgatacc gtcgagatcc
     7561 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
     7621 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
     7681 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     7741 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     7801 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     7861 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     7921 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
     7981 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     8041 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
     8101 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     8161 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     8221 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     8281 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     8341 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
     8401 aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct
     8461 ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc a
//
