LOCUS       sequence-43179-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ,
            Rinehart J, Soll D
  TITLE     Expanding the genetic code of Escherichia coli with phosphoserine.
  JOURNAL   Science. 2011 Aug 26;333(6046):1151-4.
  PUBMED    21868676
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-08-26"; volume: "333(6046)"; pages: "1151-4"
COMMENT     Sequence Label: pMYO127TAG-SepT
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(375..402)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(494..580)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(611..628)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaatccttag ctttcgctag gatctgcagg tcgactctag aggatcctgg agccgggggt
       61 gggatttgaa cccacgtaag gcggatctag agtccgctgc ctaaccccta gactaccccg
      121 gcgaattcag cgttacaagt attacacaaa gttttttatg ttgagaatat ttttttgatg
      181 gggcgccact tatttttgat cgttcgctca aagaagcggc gccagggtgg tttttctttt
      241 caccagtgag acgggcaaca gaacgccatg agcggcctca tttcttattc tgagttacaa
      301 cagtccgcac cgctgccggt agctccttcc ggtgggcgcg gggcatagat ctcaaaagag
      361 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg
      421 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat
      481 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      541 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctacg
      601 gcgcgcctta gtgatggtga tggtgatgac cagaaccacc ctggtaaccc agttctttgt
      661 atttagcagc gatgtcttta cggaacagtt ccagagcttt gttcatagca ccctgagcct
      721 aagcaccgaa gttacccgga
//
