LOCUS       sequence-431857-       13572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 13572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-SARS-CoV-2_NSP3-VA
FEATURES             Location/Qualifiers
     source          1..13572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7188..7212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7292..7315
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7352..7375
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7388..7405
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7415..7435
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7448..7468
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7481..7546
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7594..7711
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7760..8270
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8189..8207
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8292..8891
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8292..8311)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8788..8808
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9019..9252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9324..9445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9361..9380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9415..9434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9485..9620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9640..9659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9641..9659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9669..9686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9669..9685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9678..9700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9827..10282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9914..9933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10124..10145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10308..10412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10413..10481,10482..11273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10631..10650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11444..12032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11933..11952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12186..12203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12320..12341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12356..12361,12362..12379,12380..12386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12394..12410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12399..12421
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12418..12434
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12418..12434
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12453..12473
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12455..12473
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12501..12727
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12728..12908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12955..13080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcat ggcccctacc aaggtgacct tcggagacga
     1381 caccgtgatc gaggtgcagg gttacaagag cgtgaacatc accttcgagc tggacgaacg
     1441 catcgacaag gtgctgaacg aaaagtgctc cgcttacacc gtggagctgg gcaccgaggt
     1501 gaacgaattc gcctgcgtgg tggccgacgc tgtgatcaag accctgcagc cagtgtccga
     1561 actgctgacc cctctgggta tcgacctgga cgagtggtct atggctacct actacctgtt
     1621 cgacgagagc ggcgaattca agctggcctc tcacatgtac tgcagcttct accctcccga
     1681 cgaagacgag gaagagggtg actgcgaaga ggaagagttc gagccttcca ctcagtacga
     1741 gtacggcacc gaagacgact accagggcaa gcctctggaa ttcggagcca cctctgccgc
     1801 tctgcagcct gaagaggaac aggaggaaga ctggctggac gacgactccc agcagaccgt
     1861 gggtcagcag gacggctctg aagacaacca gaccaccacc atccagacca tcgtggaggt
     1921 gcagcctcag ctggagatgg aactgacccc agtggtgcag accatcgagg tgaacagctt
     1981 ctccggctac ctgaagctga ccgacaacgt gtacatcaag aacgctgaca tcgtggagga
     2041 agccaagaag gtgaaaccaa ccgtggtggt gaacgccgct aacgtgtacc tgaagcacgg
     2101 cggaggtgtg gctggagctc tgaacaaggc taccaacaac gccatgcagg tggaatccga
     2161 cgactacatc gctaccaacg gtcctctgaa ggtgggcgga tcctgcgtgc tgtctggcca
     2221 caacctggcc aagcactgcc tgcacgtggt gggaccaaac gtgaacaagg gagaggacat
     2281 ccagctgctg aagagcgcct acgaaaactt caaccagcac gaggtgctgc tggctccact
     2341 gctgtctgcc ggaatcttcg gtgctgatcc catccacagc ctgcgcgtgt gcgtggacac
     2401 cgtgaggacc aacgtgtacc tggccgtgtt cgacaagaac ctgtacgaca agctggtgag
     2461 ctccttcctg gagatgaagt ccgaaaagca ggtggagcag aagatcgccg agatccccaa
     2521 ggaggaagtg aagcctttca tcaccgaatc taagcccagc gtggagcaga gaaagcagga
     2581 cgacaagaag atcaaggcct gcgtggagga agtgaccacc accctggagg aaaccaagtt
     2641 cctgaccgag aacctgctgc tgtacatcga catcaacggc aacctgcacc ctgactccgc
     2701 taccctggtg tctgacatcg acatcacctt cctgaagaag gacgccccct acatcgtggg
     2761 cgacgtggtg caggaaggag tgctgaccgc tgtggtcatc cctaccaaga aggccggtgg
     2821 caccaccgag atgctggcca aggctctgcg taaggtgcca accgacaact acatcaccac
     2881 ctaccccggt cagggcctga acggttacac cgtggaggaa gctaagaccg tgctgaagaa
     2941 gtgcaagtct gccttctaca tcctgccctc catcatctct aacgagaagc aggaaatcct
     3001 gggcaccgtg agctggaacc tgcgcgaaat gctggcccac gctgaggaaa ccaggaagct
     3061 gatgcccgtg tgcgtggaga ccaaggccat cgtgagcacc atccagagaa agtacaaggg
     3121 catcaagatc caggagggtg tggtggacta cggcgctcgt ttctacttct acacctccaa
     3181 gaccaccgtg gcctctctga tcaacaccct gaacgacctg aacgagaccc tggtgaccat
     3241 gccactggga tacgtgaccc acggtctgaa cctggaggaa gccgctagat acatgcgttc
     3301 cctgaaggtg cctgctaccg tgagcgtgag cagccctgac gctgtgaccg cctacaacgg
     3361 atacctgacc tcctctagca agacccctga ggaacacttc atcgaaacca tcagcctggc
     3421 cggttcctac aaggactggt cttacagcgg acagtccacc cagctgggca tcgagttcct
     3481 gaagcgcgga gacaagagcg tgtactacac ctccaaccca accaccttcc acctggacgg
     3541 tgaagtgatc accttcgaca acctgaagac cctgctgtct ctgcgcgagg tgaggaccat
     3601 caaggtgttc accaccgtgg acaacatcaa cctgcacacc caggtggtgg acatgagcat
     3661 gacctacgga cagcagttcg gtcctaccta cctggacgga gctgacgtga ccaagatcaa
     3721 gccacacaac tcccacgagg gcaagacctt ctacgtgctg cctaacgatg acaccctgag
     3781 agtggaggct ttcgaatact accacaccac cgacccatcc ttcctgggca ggtacatgtc
     3841 tgccctgaac cacaccaaga agtggaagta cccccaggtg aacggactga ccagcatcaa
     3901 gtgggccgac aacaactgct acctggccac cgctctgctg accctgcagc agatcgaact
     3961 gaagttcaac ccacccgctc tgcaggacgc ttactacaga gcccgtgctg gcgaggccgc
     4021 taacttctgc gctctgatcc tggcctactg caacaagacc gtgggcgaac tgggagacgt
     4081 gagagagacc atgagctacc tgttccagca cgccaacctg gactcctgca agcgtgtgct
     4141 gaacgtggtg tgcaagacct gcggccagca gcagaccacc ctgaagggag tggaagctgt
     4201 gatgtacatg ggtaccctgt cctacgagca gttcaagaag ggcgtgcaga tcccttgcac
     4261 ctgcggaaag caggccacca agtacctggt gcagcaggaa agcccattcg tgatgatgtc
     4321 cgctcctcca gcccagtacg aactgaagca cggcaccttc acctgcgctt ccgagtacac
     4381 cggcaactac cagtgcggac actacaagca catcacctct aaggagaccc tgtactgcat
     4441 cgacggagcc ctgctgacca agtcctctga atacaagggt cccatcaccg acgtgttcta
     4501 caaggagaac agctacacca ccaccatcaa gcccgtgacc tacaagctgg acggcgtggt
     4561 gtgcaccgaa atcgacccta agctggacaa ctactacaag aaggacaact cttacttcac
     4621 cgagcagcct atcgacctgg tgccaaacca gccctaccct aacgccagct tcgacaactt
     4681 caagttcgtg tgcgacaaca tcaagttcgc tgacgacctg aaccagctga ccggatacaa
     4741 gaagcctgcc tctagagagc tgaaggtgac cttcttccca gacctgaacg gcgacgtggt
     4801 ggctatcgac tacaagcact acaccccaag cttcaagaag ggagccaagc tgctgcacaa
     4861 gcccatcgtg tggcacgtga acaacgctac caacaaggcc acctacaagc ctaacacctg
     4921 gtgcatccgt tgcctgtggt ctaccaagcc agtggaaacc tccaactctt tcgacgtgct
     4981 gaagagcgag gacgctcagg gcatggacaa cctggcctgc gaagacctga agcccgtgag
     5041 cgaggaagtg gtggagaacc caaccatcca gaaggacgtg ctggaatgca acgtgaagac
     5101 caccgaggtg gtgggagaca tcatcctgaa gccagctaac aacagcctga agatcaccga
     5161 ggaagtgggt cacaccgacc tgatggccgc ttacgtggac aacagctccc tgaccatcaa
     5221 gaagcccaac gagctgagca gggtgctggg tctgaagacc ctggctactc acggtctggc
     5281 tgctgtgaac tctgtgccat gggacaccat cgctaactac gccaagcctt tcctgaacaa
     5341 ggtggtgtcc accaccacca acatcgtgac cagatgtctg aaccgcgtgt gcaccaacta
     5401 catgccctac ttcttcaccc tgctgctgca gctgtgcacc ttcaccagaa gcaccaactc
     5461 ccgtatcaag gctagcatgc ctaccaccat cgccaagaac accgtgaaga gcgtgggaaa
     5521 gttctgcctg gaggcttcct tcaactacct gaagtctcca aacttcagca agctgatcaa
     5581 catcatcatc tggttcctgc tgctctccgt gtgcctgggt tccctgatct actctaccgc
     5641 cgctctggga gtgctgatgt ctaacctggg tatgcctagc tactgcactg gttacagaga
     5701 gggatacctg aacagcacca acgtgaccat cgccacctac tgcaccggca gcatcccatg
     5761 ctccgtgtgc ctgtctggac tggacagcct ggacacctac ccctccctgg aaaccatcca
     5821 gatcaccatc tctagcttca agtgggacct gaccgccttc ggtctggtgg ctgagtggtt
     5881 cctggcctac atcctgttca ccaggttctt ctacgtgctg ggcctggccg ctatcatgca
     5941 gctgttcttc tcctacttcg ctgtgcactt catcagcaac tcctggctga tgtggctgat
     6001 catcaacctg gtgcagatgg ctcccatctc tgccatggtg cgcatgtaca tcttcttcgc
     6061 cagcttctac tacgtgtgga agtcctacgt gcacgtggtg gacggttgca actcctctac
     6121 ctgcatgatg tgctacaagc gcaacagggc taccagggtg gagtgcacca ccatcgtgaa
     6181 cggcgtgcgc aggtccttct acgtgtacgc caacggcggc aagggattct gcaagctgca
     6241 caactggaac tgcgtgaact gcgacacctt ctgcgctggc tccaccttca tctctgacga
     6301 ggtggccaga gacctgagcc tgcagttcaa gcgtccaatc aaccccaccg accagagctc
     6361 ctacatcgtg gactctgtga ccgtgaagaa cggtagcatc cacctgtact tcgacaaggc
     6421 tggccagaag acctacgagc gccactctct gagccacttc gtgaacctgg acaacctgag
     6481 ggccaacaac accaagggca gcctgcctat caacgtgatc gtgttcgacg gaaagtccaa
     6541 gtgcgaggaa tctagcgcca agtccgccag cgtgtactac agccagctga tgtgccagcc
     6601 aatcctgctg ctggaccagg ctctggtgtc tgacgtggga gacagcgccg aagtggccgt
     6661 gaagatgttc gacgcctacg tgaacacctt ctcctctacc ttcaacgtgc ctatggagaa
     6721 gctgaagacc ctggtggcta ctgctgaggc tgaactggct aagaacgtgt ccctggacaa
     6781 cgtgctgagc accttcatct ccgccgctag gcagggtttc gtggactctg acgtggaaac
     6841 caaagacgtg gtggagtgcc tgaagctgtc tcaccagagc gacatcgaag tgaccggcga
     6901 ctcctgcaac aactacatgc tgacctacaa caaggtggag aacatgaccc caagagacct
     6961 gggagcttgc atcgactgct ccgcccgtca catcaacgct caggtggcca agtctcacaa
     7021 catcgccctg atctggaacg tgaaggactt catgagcctg tccgagcagc tgcgcaagca
     7081 gatcaggagc gccgctaaga agaacaacct gcccttcaag ctgacctgcg ctaccaccag
     7141 acaggtggtg aacgtggtga ccaccaagat cgccctgaag ggcggatacc caactttctt
     7201 gtacaaagtg gttgattcta gagcgatcgc cagagagctg ttcaacctgc tgggcgagaa
     7261 ccagcccccc gtggtgatca aggcgatcgc ctggagccac cctcagttcg agaagggagg
     7321 aggaagcggc ggaggcagcg gaggaggaag ctggagccac ccgcagttcg agaaaggcgg
     7381 aagcggccat caccatcacc atcaccctgc aggagaaaat ctgtacttcc aggggagagg
     7441 aggcagcgag aacctgtact ttcaggggcc tgcaggaaga gactacaaag accatgacgg
     7501 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagtagt tcgaattctc
     7561 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     7621 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     7681 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     7741 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     7801 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     7861 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     7921 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     7981 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     8041 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     8101 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     8161 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     8221 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac
     8281 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     8341 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     8401 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     8461 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     8521 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     8581 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     8641 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     8701 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     8761 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     8821 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     8881 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     8941 atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9001 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9241 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9301 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     9361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9421 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     9481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9541 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9601 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     9661 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     9721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     9841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10261 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    10681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    10861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    10921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    10981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    11821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    11881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    11941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12061 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12181 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12241 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    12301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    12361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    12421 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    12481 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
    12541 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
    12601 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
    12661 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
    12721 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    12781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    12841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    12901 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
    12961 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    13021 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    13081 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    13141 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    13201 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    13261 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    13321 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    13381 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
    13441 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
    13501 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
    13561 agcagtggga at
//
