LOCUS       sequence-431861-        6592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1357
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        299..712
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      1458..1645
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1513..1537)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1729..2395
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2265..2288
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             2402..2629
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             2756..3466
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(4330..5145)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4330..5145)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5053..5072
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5137..5156
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5370..5958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5859..5878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgccttg ccaacaggga gttcttcaga gacatggagg
       61 ctcaaaacga aattattgac agcctagaca tcaatagtca tacaacagaa agcgaccacc
      121 caactttggc tgataatagc gtataaacaa tgcatacttt gtacgttcaa aatacaatgc
      181 agtagatata tttatgcata ttacatataa tacatatcac ataggaagca acaggcgcgt
      241 tggactttta attttcgagg accgcgaatc cttacatcac acccaatccc ccacaagtga
      301 tcccccacac accatagctt caaaatgttt ctactccttt tttactcttc cagattttct
      361 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc
      421 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
      481 acaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
      541 ttttcttgaa aatttttttt tttgattttt ttctctttcg atgacctccc attgatattt
      601 aagttaataa acggtcatca atttctcaag tttcagtttc atttttcttg ttctattaca
      661 acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta agttttaatt
      721 acaaaagatc tatggtctgt gatacactgg tatatcatcc ctccgtgacg agattcgtca
      781 aatttctaga tggctcagct ggcagagaaa aggttctcag attactgcag tatttagcaa
      841 gatttttagc agtacagaac tcatctctct tggccaggca attacaagct caatttacca
      901 cagtcagaaa atttctgagg tttctgaagc ctttaaacca cttgcaggca gctgctaaat
      961 tctacgataa caagttggcc agcgacaacg tcgttagagt ctgcaatgtt ttgaaaaaca
     1021 tcttctttgc tgcatatttg tcgttagatc aagtcaatct tttgagaatt ttgaaagtga
     1081 ttcctgtaac cgttcttacc ggtaagaaaa tacctcgctg gtccaattgg tgttggctgt
     1141 tcggcctcct aagtggtctg gctatggatc ttcgtaagat ccaaacatct catgcccaga
     1201 ttgctgcgtt tgtcaaggca aaatcacaaa gccaaggcga tgagcatgag gatcacaaga
     1261 aggtactagg gaaggcatac caagacaggt acaccgcgtt aagaagacta ttctgggatg
     1321 ctgcagattc gttcatcgtc ctcaacaacc tagggtactt gtctagcaac gaagagtatg
     1381 ttgcgctatc cggtgttgtc acatctatcc ttggtatgca agacatgtgg aaagctacat
     1441 agggatccta actcgaggcg aatttcttat gatttatgat ttttattatt aaataagtta
     1501 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     1561 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
     1621 ctcttattga ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt
     1681 tcgctgccaa accagatgtc aacacagcta caacgcagtt cgagtttatc attatcaata
     1741 ctgccatttc aaagaatacg taaataatta atagtagtga ttttcctaac tttatttagt
     1801 caaaaaatta gccttttaat tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt
     1861 acacagaata tataacatcg taggtgtctg ggtgaacagt ttattcctgg catccactaa
     1921 atataatgga gcccgctttt taagctggca tccagaaaaa aaaagaatcc cagcaccaaa
     1981 atattgtttt cttcaccaac catcagttca taggtccatt ctcttagcgc aactacagag
     2041 aacaggggca caaacaggca aaaaacgggc acaacctcaa tggagtgatg caacctgcct
     2101 ggagtaaatg atgacacaag gcaattgacc cacgcatgta tctatctcat tttcttacac
     2161 cttctattac cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag
     2221 ttccctgaaa ttattcccct acttgactaa taagtatata aagacggtag gtattgattg
     2281 taattctgta aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt
     2341 ttagttttaa aacaccaaga acttagtttc gaataaacac acataaacaa acaaaagatc
     2401 tatgcagatt ttcgttaaaa ctttaactgg taaaacaatt actttagaag ttgagtcatc
     2461 tgatactatc gataatgtta aatctaagat ccaggataag gaaggtattc ctccagatca
     2521 acaaagattg attttcgcag gtaagcagtt ggaggacggt aggactttgt ctgattataa
     2581 catacagaaa gaatctactt tgcacttagt cttaagatta aggggaggtt atcatggttc
     2641 tggtgcctgg ttgttacctg tatcattggt taaaagaaag accacattgg caccaaatac
     2701 tcaaactgcc tctccaagag ccttggcaga ctctttaatg cagagatcag gttcttctaa
     2761 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
     2821 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac
     2881 cttaaaattg atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
     2941 tttaggttat ggtttgcaat gttttgctag atacccagat catatgaaac aacatgactt
     3001 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
     3061 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
     3121 cgaattaaaa ggtattgatt ttaaagaagg tggtaacatt ttaggtcaca aattggaata
     3181 caactataac tctcacaatg tttacatcac tgctgacaaa caaaagaatg gtatcaaagc
     3241 taacttcaaa attagacaca acattgaaga tggtggtgtt caattagctg accattatca
     3301 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccta
     3361 tcaatctgcc ttatccaaag atccaaacga aaagagagat cacatggtct tgttagaatt
     3421 tgttactgct gctggtatta cccatggtat ggatgaattg tacaaagggt ccttgggaag
     3481 aggtagaaga tccaaattgt aaggatccta actcgagagt gcttttaact aagaattatt
     3541 agtcttttct gcttattttt tcatcatagt ttagaacact ttatattaac gaatagttta
     3601 tgaatctatt taggtttaaa aattgataca gttttataag ttactttttc aaagactcgt
     3661 gctgtctatt gcataatgca ctggaagggg aaaaaaaagg tgcacacgcg tggctttttc
     3721 ttgaatttgc agtttgaaaa atgctgagca gttacagaga tgttacgaac cactagtgca
     3781 ctgcagtaca tgttatacca tagtagtagt ttcaataata tattccacta cttatatgtg
     3841 ttacccgcat tagaactctt attggtggcg aaaatcgatg gcaataaaga acggaagggg
     3901 tttaatagtt gtatgcttaa catatttcga tttaaatata taagaaacgt cggtagcaca
     3961 acaattaact cattatttag gtatggcgga aatacctgat gaaaccatcc agcagttcat
     4021 ggcattgacc aatgtgtcgc ataacatagc cgttcaatat ctctctgaat ttggagattt
     4081 aaatgaagca ctaaattcct attatgcttc tcaaacggat gaccaaaagg atagaagaga
     4141 ggaagcacat tggaacagac agcaggagaa ggccctcaag caagaagcct tctccaccaa
     4201 ctcttcgaat aaagccataa atacggagca cgttggtggg ttatgtccaa aaccaggatc
     4261 ctcacaaggt agcaacgagt acttgaaaag ccgagcggcc gcgtgttaca accaattaac
     4321 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     4381 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     4441 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     4501 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     4561 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
     4621 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     4681 tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac
     4741 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     4801 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     4861 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     4921 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     4981 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     5041 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     5101 taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     5161 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     5221 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
     5281 gttgaaggat cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg
     6061 gccgccaatt gtgaatgaag tagcgacaga aaaaaagctt ttatttcgag ttcgcatcat
     6121 tgtcactttc ctgctttttc ttggcgtact gtctatctaa gatagtcttt aatatagcgt
     6181 cctcgccata ttcttcttcc tcaattttct tccatctctt ttctacatca gctctcttta
     6241 ttctactttc aatgacagca cgtttcttgt tattcaaact tgcctccttg agacacttgt
     6301 tcaaagcatc cttttcgttg ttacaaaggc caaatattct cttgtaatat tccttttgat
     6361 ggcatttatc gagtgcattg atgaagtcca aacaggctaa aatgaagaag gggagaatac
     6421 aatgttagta agtcggagca cagaaaaagg caaatatatg aagtaaaatc tggatactta
     6481 caatgaaaac gttcagcttc taactgtgga tgcatgttgt agtacttgtc ttatcttgct
     6541 tttgttcaac tgcacttgta aatcagtgac cctgaattcg catctagact ga
//
