LOCUS       sequence-431871-        5730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1535
FEATURES             Location/Qualifiers
     source          1..5730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        299..712
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      2586..2773
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2641..2665)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3468..4283)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3468..4283)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4191..4210
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4275..4294
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4508..5096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4997..5016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgccttg ccaacaggga gttcttcaga gacatggagg
       61 ctcaaaacga aattattgac agcctagaca tcaatagtca tacaacagaa agcgaccacc
      121 caactttggc tgataatagc gtataaacaa tgcatacttt gtacgttcaa aatacaatgc
      181 agtagatata tttatgcata ttacatataa tacatatcac ataggaagca acaggcgcgt
      241 tggactttta attttcgagg accgcgaatc cttacatcac acccaatccc ccacaagtga
      301 tcccccacac accatagctt caaaatgttt ctactccttt tttactcttc cagattttct
      361 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc
      421 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
      481 acaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
      541 ttttcttgaa aatttttttt tttgattttt ttctctttcg atgacctccc attgatattt
      601 aagttaataa acggtcatca atttctcaag tttcagtttc atttttcttg ttctattaca
      661 acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta agttttaatt
      721 acaaaagatc tatggacgta ggaagttgct cagtgggaaa taatccgctt gcgcagttgc
      781 acaaacatac tcagcagaac aaatcgcttc agtttaatca gaagaataat gggcgtctta
      841 atgagtcacc tctacagggt accaacaagc caggtattag tgaggctttt atatccaatg
      901 ttaatgctat ttcacaagaa aacatggcga atatgcaaag gttcataaac ggagaaccac
      961 tgatcgatga taaaagaaga atggaaatag ggccatcctc aggcaggctt ccaccttttt
     1021 caaacgtaca ttctctacag acttcagcaa acccaaccca aattaaggga gtgaacgata
     1081 tatctcattg gtcacaggaa tttcaaggta gtaatagtat tcaaaataga aacgcggata
     1141 caggaaattc agaaaaggca tggcagcgtg gctcaacaac cgcatcaagc cggtttcagt
     1201 accctaatac catgatgaat aactatgctt atgcttctat gaacagtctt agtggatcaa
     1261 ggctccaatc gcctgctttc atgaatcaac aacagtctgg tcgttctaaa gaaggagtca
     1321 atgagcaaga acaacaaccc tggacagatc agtttgaaaa gctggaaaaa gaagtctcag
     1381 aaaacttgga cataaatgat gaaatagaga aggaggaaaa tgtgagtgaa gtagaacaaa
     1441 acaaaccaga aactgttgag aaggaagaag gagtatatgg agatcagtat caatctgatt
     1501 tccaagaagt gtgggatagc atacacaagg acgctgaaga agtcttgcca tccgaattag
     1561 ttaatgatga cctcaatcta ggagaagact acttgaaata tctcggcggt agagtaaatg
     1621 ggaacatcga gtatgctttt caatctaaca acgaatattt taataatcct aatgcttata
     1681 aaattggctg cctactgatg gaaaacggag ccaaattgag cgaggcagcg ctagcatttg
     1741 aagctgctgt taaagaaaaa ccggaccatg tggatgcatg gctaagattg ggtctagtac
     1801 aaacccagaa tgaaaaagag ttgaacggca taagcgccct cgaagaatgt ttaaagttag
     1861 acccaaagaa tctggaggca atgaaaactt tagcgataag ttatataaac gaaggttatg
     1921 atatgagcgc cttcacaatg ctggataaat gggcagaaac taagtacccg gaaatttggt
     1981 caaggatcaa gcaacaagat gacaaatttc aaaaagagaa agggtttacc catattgata
     2041 tgaacgctca tatcacaaag caatttttgc aactagcaaa caatttaagc acaatagatc
     2101 ctgaaataca actatgcttg ggactcttat tttacacgaa agatgatttt gacaaaacca
     2161 tagattgctt tgaaagtgcg ttgagggtga atcctaatga cgaactcatg tggaatagat
     2221 taggggcttc attggccaat tccaatagat cagaggaagc aatccaagcc tatcataggg
     2281 cactacaact aaaaccttct tttgttagag ctcgctataa tctggcggta tcatccatga
     2341 atataggctg tttcaaagaa gcagcaggct acttattaag tgttctaagt atgcatgaag
     2401 tgaacactaa taataaaaaa ggagatgttg gatctctctt gaatacgtac aatgatactg
     2461 ttatagagac tttgaagaga gtttttatag cgatgaatag agatgattta cttcaagaag
     2521 tgaagccagg catggacctg aaaagattta aaggagaatt ttcgttttga ggatcctaac
     2581 tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     2641 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     2701 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     2761 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc gctgccaaac
     2821 cagatgtcaa cacagctaca acgactagag atctatgtga ggatcctaac tcgagatcga
     2881 gctgagcagt tacagagatg ttacgaacca ctagtgcact gcagtacatg acatgtatgg
     2941 gttgaaaata tttagaggat gctaaaagtc ccgtctaaaa tagaggaagt catggattac
     3001 attatcacct ttattgtcct gttccgtctg gctaatttgg gacgaggaac gtttgtctcc
     3061 attcgcaacc ttttgaaacc atatgaacgg cggctaatct gacagatgtc ttatatttct
     3121 aggattgtag aaagcctcct catcaaacca ccttgtttga aaaaggcact agtgcggttg
     3181 ctttacttga tctagtattc tcctaccttg gagcggtccc atatcaacta aattcagttt
     3241 tctgactgag ttaaaaacta atgtagcgac accacttcca ttggtgcctc cgttattttt
     3301 tttttgtatg ttaatgctaa ataatacacc gctatgtatt tcagggcact acttctacac
     3361 atcaacggta ctaaacattt cgctcaagat cggtccgctt tcacgtattt ggatgctatg
     3421 caatgttgcc gagcggccgc gtgttacaac caattaacca attctgatta gaaaaactca
     3481 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     3541 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     3601 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     3661 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     3721 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     3781 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagg
     3841 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     3901 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     3961 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     4021 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     4081 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     4141 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     4201 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac
     4261 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     4321 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
     4381 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gtcatgacca
     4441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5161 ttcctgcgtt atcccctgat tctgtggata accgtgcggc cgccaattga aaaaggccac
     5221 tactgagcca agcgcagaac cagatgagcc attcattgga tacttgggtg acgttactgc
     5281 atctataaac caagatataa aagaatatgg tcgcagaaga acagtaatac ctacaagcga
     5341 tttagtagcg tcgttctaca ccaaaaagac gcctgttttt aaggtcaagt tcagcagaag
     5401 caatttagcc cttgcaggag gtgctttcag gccataattc acactgaaaa aaataacagc
     5461 atggaagtga tatgtaataa gtataaccta atccgatatg taactattag atgctttttc
     5521 gacatcgtac ttgacctgct cggcggttat catagcgtct tctttagcct tttttttttc
     5581 gctcagtcat cggggccaaa acaagaaagg cgaagaacag aaaaatgaaa aatgtaactg
     5641 gttgttgtaa aaaccttcca gagaaataag atgaaatgta tgttgctggc acgaacgaag
     5701 acaaataccc tgaattcgca tctagactga
//
