LOCUS       sequence-431873-        7065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB711
FEATURES             Location/Qualifiers
     source          1..7065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1433..1620
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1488..1512)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2096..2503
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2504..3598
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(4629..5444)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4629..5444)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5352..5371
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5436..5455
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5669..6257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6158..6177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgg tctgtgatac
      721 actggtatat catccctccg tgacgagatt cgtcaaattt ctagatggct cagctggcag
      781 agaaaaggtt ctcagattac tgcagtattt agcaagattt ttagcagtac agaactcatc
      841 tctcttggcc aggcaattac aagctcaatt taccacagtc agaaaatttc tgaggtttct
      901 gaagccttta aaccacttgc aggcagctgc taaattctac gataacaagt tggccagcga
      961 caacgtcgtt agagtctgca atgttttgaa aaacatcttc tttgctgcat atttgtcgtt
     1021 agatcaagtc aatcttttga gaattttgaa agtgattcct gtaaccgttc ttaccggtaa
     1081 gaaaatacct cgctggtcca attggtgttg gctgttcggc ctcctaagtg gtctggctat
     1141 ggatcttcgt aagatccaaa catctcatgc ccagattgct gcgtttgtca aggcaaaatc
     1201 acaaagccaa ggcgatgagc atgaggatca caagaaggta ctagggaagg cataccaaga
     1261 caggtacacc gcgttaagaa gactattctg ggatgctgca gattcgttca tcgtcctcaa
     1321 caacctaggg tacttgtcta gcaacgaaga gtatgttgcg ctatccggtg ttgtcacatc
     1381 tatccttggt atgcaagaca tgtggaaagc tacataggga tcctaactcg aggcgaattt
     1441 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     1501 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
     1561 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     1621 catgccgagc aaatgcctgc aaatcgctcc ccatttcgct ggaaatctgc tcgtcagtgg
     1681 tgctcacact gacgaatcat gtacagatca taccgatgac tgcctggcga ctcacaacta
     1741 agcaagacag ccggaaccag cgccggcgaa caccactgca tatatggcat atcacaacag
     1801 tccacgtctc aagcagttac agagatgtta cgaaccacta gtgcactgca gtacatcgag
     1861 gagaacttct agtatatcta catacctaat attattgcct tattaaaaat ggaatcccaa
     1921 caattacatc aaaatccaca ttctcttcaa aatcaattgt cctgtacttc cttgttcatg
     1981 tgtgttcaaa aacgttatat ttataggata attatactct atttctcaac aagtaattgg
     2041 ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat atcttgaccg cagttaactg
     2101 tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac cattattttt
     2161 ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat tcgatgactg
     2221 gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt
     2281 gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat agagaagcgt
     2341 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
     2401 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
     2461 ttcagcaata tatatatata tatttcaagg atataccatt ctaatgtctg cccctaagaa
     2521 gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc acagccgaag ccattaaggt
     2581 tcttaaagct atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat
     2641 tggtggtgct gctatcgatg ctacaggtgt tccacttcca gatgaggcgc tggaagcctc
     2701 caagaaggct gatgccgttt tgttaggtgc tgtgggtggt cctaaatggg gaaccggtag
     2761 tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt acgccaactt
     2821 aagaccatgt aactttgcat ccgactctct tttagactta tctccaatca agccacaatt
     2881 tgctaaaggt actgacttcg ttgttgtcag agaattagtg ggaggtattt actttggtaa
     2941 gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt gaacaataca ccgttccaga
     3001 agtgcaaaga atcacaagaa tggccgcttt catggcccta caacatgagc caccattgcc
     3061 tatttggtcc ttggataaag ctaatgtttt ggcctcttca agattatgga gaaaaactgt
     3121 ggaggaaacc atcaagaacg aatttcctac attgaaggtt caacatcaat tgattgattc
     3181 tgccgccatg atcctagtta agaacccaac ccacctaaat ggtattataa tcaccagcaa
     3241 catgtttggt gatatcatct ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt
     3301 gccatctgcg tccttggcct ctttgccaga caagaacacc gcatttggtt tgtacgaacc
     3361 atgccacggt tctgctccag atttgccaaa gaataaggtc aaccctatcg ccactatctt
     3421 gtctgctgca atgatgttga aattgtcatt gaacttgcct gaagaaggta aggccattga
     3481 agatgcagtt aaaaaggttt tggatgcagg tatcagaact ggtgatttag gtggttccaa
     3541 cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa
     3601 agattctctt tttttatgat atttgtacat aaactttata aatgaaattc ataatagaaa
     3661 cgacacgaaa ttacaaaatg gaatatgttc atagggtaga cgaaactata tacgcaatct
     3721 acatacattt atcaagaagg agaaaaagga ggatgtaaag gaatacaggt aagcaaattg
     3781 atactaatgg ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag
     3841 gaggagggca ccacacaaaa agttaggtgt aacagaaaat catgaaacta tgattcctaa
     3901 tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa
     3961 tgacctgacc atttgatgga gtttaagtca ataccttctt gaaccatttc ccataatggt
     4021 gaaagttccc tcaagaattt tactctgtca gaaacggcct taacgacgta gtcgagagta
     4081 ctcgtatcgc atgtcggtgc gacacgaaat tacaaaatgg aatatgttca tagggtagac
     4141 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg
     4201 aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt
     4261 taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc
     4321 atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca
     4381 acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg
     4441 aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt
     4501 aacgacgtag tcgacctcct cttcagtact aaatctacca ataccaaatc tgatggaaga
     4561 atgggctaat gcatcatcct tacccagcgc cgagcggccg cgtgttacaa ccaattaacc
     4621 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     4681 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     4741 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     4801 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     4861 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     4921 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     4981 cgtgattgcg cctgagcgag gcgaaatacg cgatcgctgt taaaaggaca attacaaaca
     5041 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     5101 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     5161 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     5221 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     5281 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     5341 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     5401 aatcgcggcc tggagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     5461 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     5521 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     5581 ttgaaggatc agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
     6361 ccgccaatac ccagaagaac agtaaaataa agcaaggtac gtgaaattaa tatttttaaa
     6421 tggttctaac cgatgccgaa gaactgcgca gtccggttat aacgtctgac atgtcctttt
     6481 ttgatttgga atccaaccac tcaagtgact ctgttcattt actttgcgaa aaatataccc
     6541 acaaattgcc catcgaaagt gaatcgcaaa ccaccttcag actggcaccg acaaagcaaa
     6601 gattatacag acagagtact ttatacgtac cgttaagtct caagcaaagg gttttcttat
     6661 ttactgaacg ggtaaagagt atctgggccg gcttgccaag atgcaaaccg aataagtatt
     6721 tcaaagttgc atttgcctta gccgtcctga caccattggc tatttggata ttttatattg
     6781 actttcgtgt acattgatca catcgactgt tctattggca aatgaaccac gggcattgac
     6841 tatttttcag gttactacta tatattatca tcacgggcaa ggattgtacc ctgaattcgc
     6901 atctagactg atgagacgtg gtagagccac aaacagccgg tacaagcaac gatctccagg
     6961 accatctgaa tcatgcgcgg atgacacgaa ctcacgacgg cgatcacaga cattaaccca
     7021 cagtacagac actgcgacaa cgtggcaatt cgtcgcaata caacg
//
