LOCUS       sequence-431874-        8094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1335
FEATURES             Location/Qualifiers
     source          1..8094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2462..2649
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2517..2541)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        3125..3532
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3533..4627
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5658..6473)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5658..6473)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6381..6400
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6465..6484
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7187..7206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatga gtgaaaccag
      721 ctcaagtcgc aggagcgcga gtaaagacgc tgtcaaatct tacttcgcag gcaaatacaa
      781 taaagtgttg gattctatac tggaggcaga agcagcaata tcaaaatcgc caactgtagc
      841 agaagatcta tcaggaagct cctccagcgg aaattctgag atgagccatc cttcgctaac
      901 cgcctcctcc gcaacaagcc aaggcattag caaaaaggaa ctactccaac aaattgctgg
      961 ttcattattc agtacgtcca ttgagcgcct gaagactgcc cactcatcag aagtatcaag
     1021 tactccggaa tattcagtaa atgattcgta tggagagcaa gagtgcaggg agtgcgatgg
     1081 tgttttcaag tgttcagcgc actttgaggg cgcgcccgaa tattatgacg atgaaaccga
     1141 atcagggcca gctctagaac ctatgacttc caatagtgaa aaagatcctt ttatcgatgt
     1201 atttttggat aaactaatat cgaggttggt ccctgaaaag ctaccagaaa gagagcattt
     1261 tagtagcaaa accaccattg agcatgatct agacacagga agggttcccg tgttttcagc
     1321 aactacattg ggtagtaatt tcaaaaaatt gtctaagaaa atgggctcaa tatttgaact
     1381 ccaggactcc atagttcgac tgctgacttg gagaaaccct accgggacgg tgacgtcgtt
     1441 aatattattc actttgatat gttttaatcc aatgtacttg gtaattttgc ctatattccg
     1501 ctttgtctat ggtattgtag ttcctggcta tgtgcgtaaa catcctttac aaaggagtat
     1561 atatcctctg aaacggaatc acggatcatc attgttatat gatgtgtgct atgagggaaa
     1621 aaatgagtac agctacggtc aacagttttt ctccaaatct tttatggaca ctttggaatc
     1681 aagaaatcaa gaaatagacg aaatatcaga attagataaa agaaccgaaa acacagggga
     1741 attgaaacaa ggaatgaaag tactaattaa tttacgcgat atgcaaaaca tgacctctgg
     1801 tacccttcat gttattgagg caatcaatag ttttcttcgt aagtcttcta gttttcaaaa
     1861 cgaagagtgc tccactaaac gtttttttac aggatttctt ttgattgttt tcttaaaaat
     1921 attatcacca tttgtgaatt ggagctatgt atgtagcata ttcgcctggt gcttactgat
     1981 atatatgcat ccaagagctc atccaaaaat aataagtttt ttcaaaacag gaacaatggg
     2041 aaaagaatac aaaaacttga aaaaaagaga acaccaggcg cttaacatga tattcgatga
     2101 acaaccggaa actaaattca tcgagatttt tgaaatatat aaaaaggcct tactgccgaa
     2161 cgactggaag tttttccgat attcaaatag aattttcgat cctcaagatc cgtatagaag
     2221 agcacagcaa ttcccaccgg gagtagattc tttggctgat gttattcccc ccacaggctg
     2281 gtcatttgat cctaattttg agtggaaaat tgacaatgat gtggataggt gggtggtaga
     2341 acgtgggttg aatctgccaa ttaccggcga attcttattt gatccaatgt tcaagaggag
     2401 gagactgata catcgtgtta ttaagaatgc aacaccagta gcataaggat cctaactcga
     2461 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     2521 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     2581 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     2641 ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcgctg gaaatctgct
     2701 cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact gcctggcgac
     2761 tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat atatggcata
     2821 tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag tgcactgcag
     2881 tacatcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     2941 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     3001 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     3061 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     3121 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     3181 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     3241 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     3301 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     3361 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     3421 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     3481 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     3541 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     3601 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     3661 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     3721 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     3781 aaccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     3841 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     3901 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     3961 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     4021 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     4081 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     4141 aaaaactgtg gaggaaacca tcaagaacga atttcctaca ttgaaggttc aacatcaatt
     4201 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     4261 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     4321 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     4381 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     4441 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     4501 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     4561 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     4621 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     4681 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     4741 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     4801 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     4861 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     4921 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     4981 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     5041 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     5101 tcgagagtac tcgtatcgca tgtcggtgcg acacgaaatt acaaaatgga atatgttcat
     5161 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
     5221 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
     5281 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
     5341 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
     5401 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
     5461 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
     5521 aacggcctta acgacgtagt cgacctcctc ttcagtacta aatctaccaa taccaaatct
     5581 gatggaagaa tgggctaatg catcatcctt acccagcgcc gagcggccgc gtgttacaac
     5641 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     5701 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     5761 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     5821 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     5881 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     5941 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     6001 ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa
     6061 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     6121 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     6181 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     6241 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     6301 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     6361 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     6421 ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc
     6481 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     6541 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     6601 tttgctgagt tgaaggatca gtcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7381 accgtgcggc cgccaatacc cagaagaaca gtaaaataaa gcaaggtacg tgaaattaat
     7441 atttttaaat ggttctaacc gatgccgaag aactgcgcag tccggttata acgtctgaca
     7501 tgtccttttt tgatttggaa tccaaccact caagtgactc tgttcattta ctttgcgaaa
     7561 aatataccca caaattgccc atcgaaagtg aatcgcaaac caccttcaga ctggcaccga
     7621 caaagcaaag attatacaga cagagtactt tatacgtacc gttaagtctc aagcaaaggg
     7681 ttttcttatt tactgaacgg gtaaagagta tctgggccgg cttgccaaga tgcaaaccga
     7741 ataagtattt caaagttgca tttgccttag ccgtcctgac accattggct atttggatat
     7801 tttatattga ctttcgtgta cattgatcac atcgactgtt ctattggcaa atgaaccacg
     7861 ggcattgact atttttcagg ttactactat atattatcat cacgggcaag gattgtaccc
     7921 tgaattcgca tctagactga tgagacgtgg tagagccaca aacagccggt acaagcaacg
     7981 atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc gatcacagac
     8041 attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac aacg
//
