LOCUS       sequence-431875-       11167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex5, Pex2, Pex17, and Pex10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 11167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1577
FEATURES             Location/Qualifiers
     source          1..11167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        299..712
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      2586..2773
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2641..2665)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8905..9720)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8905..9720)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9628..9647
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9712..9731
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9945..10533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10434..10453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgccttg ccaacaggga gttcttcaga gacatggagg
       61 ctcaaaacga aattattgac agcctagaca tcaatagtca tacaacagaa agcgaccacc
      121 caactttggc tgataatagc gtataaacaa tgcatacttt gtacgttcaa aatacaatgc
      181 agtagatata tttatgcata ttacatataa tacatatcac ataggaagca acaggcgcgt
      241 tggactttta attttcgagg accgcgaatc cttacatcac acccaatccc ccacaagtga
      301 tcccccacac accatagctt caaaatgttt ctactccttt tttactcttc cagattttct
      361 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc
      421 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
      481 acaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
      541 ttttcttgaa aatttttttt tttgattttt ttctctttcg atgacctccc attgatattt
      601 aagttaataa acggtcatca atttctcaag tttcagtttc atttttcttg ttctattaca
      661 acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta agttttaatt
      721 acaaaagatc tatggacgta ggaagttgct cagtgggaaa taatccgctt gcgcagttgc
      781 acaaacatac tcagcagaac aaatcgcttc agtttaatca gaagaataat gggcgtctta
      841 atgagtcacc tctacagggt accaacaagc caggtattag tgaggctttt atatccaatg
      901 ttaatgctat ttcacaagaa aacatggcga atatgcaaag gttcataaac ggagaaccac
      961 tgatcgatga taaaagaaga atggaaatag ggccatcctc aggcaggctt ccaccttttt
     1021 caaacgtaca ttctctacag acttcagcaa acccaaccca aattaaggga gtgaacgata
     1081 tatctcattg gtcacaggaa tttcaaggta gtaatagtat tcaaaataga aacgcggata
     1141 caggaaattc agaaaaggca tggcagcgtg gctcaacaac cgcatcaagc cggtttcagt
     1201 accctaatac catgatgaat aactatgctt atgcttctat gaacagtctt agtggatcaa
     1261 ggctccaatc gcctgctttc atgaatcaac aacagtctgg tcgttctaaa gaaggagtca
     1321 atgagcaaga acaacaaccc tggacagatc agtttgaaaa gctggaaaaa gaagtctcag
     1381 aaaacttgga cataaatgat gaaatagaga aggaggaaaa tgtgagtgaa gtagaacaaa
     1441 acaaaccaga aactgttgag aaggaagaag gagtatatgg agatcagtat caatctgatt
     1501 tccaagaagt gtgggatagc atacacaagg acgctgaaga agtcttgcca tccgaattag
     1561 ttaatgatga cctcaatcta ggagaagact acttgaaata tctcggcggt agagtaaatg
     1621 ggaacatcga gtatgctttt caatctaaca acgaatattt taataatcct aatgcttata
     1681 aaattggctg cctactgatg gaaaacggag ccaaattgag cgaggcagcg ctagcatttg
     1741 aagctgctgt taaagaaaaa ccggaccatg tggatgcatg gctaagattg ggtctagtac
     1801 aaacccagaa tgaaaaagag ttgaacggca taagcgccct cgaagaatgt ttaaagttag
     1861 acccaaagaa tctggaggca atgaaaactt tagcgataag ttatataaac gaaggttatg
     1921 atatgagcgc cttcacaatg ctggataaat gggcagaaac taagtacccg gaaatttggt
     1981 caaggatcaa gcaacaagat gacaaatttc aaaaagagaa agggtttacc catattgata
     2041 tgaacgctca tatcacaaag caatttttgc aactagcaaa caatttaagc acaatagatc
     2101 ctgaaataca actatgcttg ggactcttat tttacacgaa agatgatttt gacaaaacca
     2161 tagattgctt tgaaagtgcg ttgagggtga atcctaatga cgaactcatg tggaatagat
     2221 taggggcttc attggccaat tccaatagat cagaggaagc aatccaagcc tatcataggg
     2281 cactacaact aaaaccttct tttgttagag ctcgctataa tctggcggta tcatccatga
     2341 atataggctg tttcaaagaa gcagcaggct acttattaag tgttctaagt atgcatgaag
     2401 tgaacactaa taataaaaaa ggagatgttg gatctctctt gaatacgtac aatgatactg
     2461 ttatagagac tttgaagaga gtttttatag cgatgaatag agatgattta cttcaagaag
     2521 tgaagccagg catggacctg aaaagattta aaggagaatt ttcgttttga ggatcctaac
     2581 tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     2641 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     2701 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     2761 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc gctgccaaac
     2821 cagatgtcaa cacagctaca acgggatatc gtaaacaaag gcgttaccat agaaatgtac
     2881 tgattggcag aattactctt caggagaatc tttcatacaa aggtattcca ttggggaaaa
     2941 tctcgttacc aagtcaatgc tgaactttct atggcctttg tttactatcg ttaattattt
     3001 tacgaccact tctgggtaga aatatttcgt agccctggaa cgagcttgtt tacgcgtttt
     3061 atcccattat atggcaccca aatcaaattt aaaaagaaaa aacgcgtaaa cagtgtcggg
     3121 taagttcatc ctctgttact ttaattgctt ctttttttga aattctaagt aaacgcgtca
     3181 ttttgatcct caggacacag aaatccttgc agaatcttat tgggtgttga atagaggacg
     3241 cgtaaaaacg atatggaaat ttttttcata tagtgtagaa agaataggtt ggcgtaggta
     3301 gtttcgtgtt tgatagaaac ctccaacaaa gtctgcaact cacgttttag aataacaagt
     3361 ttagagttta tcttgttgcc tttgttaagt cagtaccatt gaataaaaat tatataaagg
     3421 agctaatatt tcattgttgg aaaattactc taccataatt gaagcatatc tcatcctttt
     3481 catccttttc aacgcaagag agacaccaac gaacaacact ttatttgttg atatattaac
     3541 atcagatcta tgtctcgagt tgcacagctc gactcgatag cgcttgataa agaattgtat
     3601 ggacagtttt ggtccgagtt caatgcagca ttcaatacga gtgaacacaa agaggaatgg
     3661 gagttggcac tgaacacagt tgtgtttatg tgcgcgacga ggttcctgcc acactatggc
     3721 tctagctgta cgtatggatc cgcactcagt ggcgtggttt ttcagtgcag gaaacgcacc
     3781 ttgtatgtcg tcactgtctt ggcagggtac gtgtggaaga agatcacaca tattattttt
     3841 aatgggccgc actgtgggaa ccagatgatg tggctgaagc tgtacaaatg ggttaattta
     3901 ttgtatcacg gctgtgatgt aaccaacttc ctgaggtttc tagcagcgga agggcccaat
     3961 gcgagggcgt ttttgtcgcc gttataccgg gcttttaatg tccattccac aaggttgatt
     4021 cgggatggtt ctgcaattgc ctctgaattt tattcgaact ctgtattcgc tgggttagaa
     4081 taccagaata gacaattgct atggaatgca ctattagaac tcttttccaa tacactactt
     4141 acgaaaagag gactcttgac ctttgttaaa aaaccaccaa gatctcgttc tacgactact
     4201 tataagacag tttgcccccg ttgcggcggt tttccaacca acccatacca gatcgcttgt
     4261 tgtcgtgcga actactgtta cgtgtgcgtt gtaaaggcgt tggagtggtc tatgtgcgat
     4321 gcctgtggat cctctgggag actgaccgcc tcaccagtgt actaaggatc ctaactcgag
     4381 gtgaatttac tttaaatctt gcatttaaat aaattttctt tttatagctt tatgacttag
     4441 tttcaattta tatactattt taatgacatt ttcgattcat tgattgaaag ctttgtgttt
     4501 tttcttgatg cgctattgca ttgttcttgt ctttttcgcc acatgtaata tctgtagtag
     4561 atacctgata cattgtggat gctgagtgaa attttagtta ataatgctgg atgcacacac
     4621 tggcttaaga tgacaacgaa ggcaagccca gaaaaatatc gcaagcacct ttggtcttac
     4681 agtgccaact tttggcctgc cgacgttaag agtacaaagc tgatggcaat gtacgacaag
     4741 ataacagagt ctcaaaagaa gtgaaacaat ttttcttcac cacattttcc attgttcctt
     4801 ccccccataa ctataaacgt atttatgtat atatatttgc gtgtaagtgt gtgtactata
     4861 gggcaccgta aagtaataat gcttaattag ttactactat gaccatataa gaggtcatac
     4921 tgtatgaagc cacaaagcag atagatcaat catgtttaac gaaaactgtt aatcgaagat
     4981 tatttctttt tttttttctc tttccttttt acaaagaaaa ttttttttgc gctttttgcc
     5041 atcaccatcg caagttctgg gacaattgtt ctctttcgct ccagttccaa ggaaagaggt
     5101 ttctgtttta cttaatagaa agtgtcatct tgtattttat atctcttctt tcttgtgtaa
     5161 aattctttag ttttgatttt gtatttttag gacagtgagc tacgaagtaa catttttact
     5221 taataaccgt ttgaagcata gagcaggccc tggtatcacc acctaatatc tggcttttta
     5281 ttcaataaaa actcaaaaaa aaaaatccaa aaaaaactaa aaaaccaata aaaataaaag
     5341 atctatgaca tcgattaaca gttttcccag gaatattgac tggccttcca acataggcat
     5401 taaaaaaata gagggaacta atccaacggt gaatgccatc aagggcttgt tatataatgg
     5461 tggatcaatt tatgcatttc tatattttgt tattgctatg tttgttgaac caacgctaca
     5521 aaagcagtac cagcagagaa atgatttttc cttgtttgtt ttgttgcgtt tgagaagaat
     5581 catagcgcaa ttgcaaaaac gactggtgat gaccccagta tcgtcgttgg ggttcaacga
     5641 acagaataac tttgtggaga ggtccactca aacttcagac gacaatataa tacgagaaga
     5701 taatagccat tgggctgaaa tgatttatca actgcaaaat atgaaacaag aattacagta
     5761 ttttaacaga tcctcaggcc aaccatctga aagtatagac gattttgtct ttcaaattaa
     5821 gatggtgact gaccaggttg agttaacaga taggtcccga gctttctcaa ataaatcaag
     5881 aaatattata cagggaatcc gagaaatcaa aggttggttt gtgaatggcc aagtgccaag
     5941 gtaaggatcc taactcgaga gtgcttttaa ctaagaatta ttagtctttt ctgcttattt
     6001 tttcatcata gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta
     6061 aaaattgata cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg
     6121 cactggaagg ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa
     6181 aaatgctggt tctgtatggg cacagacaac ctaaacgact agagatctat gtgaggatcc
     6241 taactcgaga tcgagctggg tagactaccc atgagtcaca atgaacgacg atggcttctt
     6301 atctcacttc aatagtactt tccaccggtt atacttccgg cttttcccta ttaatacaag
     6361 ctacaatttc aatgggtggc aaataatgtg tagaatagaa aataagccga cagggtaata
     6421 aagaaaattt ttagaaaaaa aaggttagat ggcttattta agttacaggc tagcgaaaaa
     6481 aggaacttca gggcaagtaa agtgtttgat tgggcactag catggcttat aaaggcgagc
     6541 aattgtcgaa actaattaat gttgtacgga ctattgctgt catctcgtgg taaatgcgtg
     6601 ttccaggtcg aatactactt gcacacaggc gagcggggcc ccataaaagt gttgccgatt
     6661 tgttaagttg tcttttcggt ttttctactc tgttattcct tacttccctt tttaagaact
     6721 ctttttatcc ttcatttagg atcttgcacg tttccgcctc atcacttgaa ttaaaacatg
     6781 tctctgtcag taaaccttgg cgtttctatt gttcttcata gttcaacttt tattattacc
     6841 cgccctgcgc gtttacattt ttccagcaac agccagcgaa aaattagaaa atctggttgt
     6901 tgacacctca agaacaaggg caattagcct cagcgtcgaa tatagatcat attagaatac
     6961 ctatagctcc atcaaaagaa atacacaaga tctatgaaga atgataataa gttgcaaaag
     7021 gaagcactta tgcgactatc gcagctaaga tttccatttg ccgatgctcc aagtatagta
     7081 caagcgcatc aaaaagatga acaaatccag ggccttttga ttatgaaagt gactgaattg
     7141 tgcaaattga taaagagtca attgtttgtt aactcttacc ctaaggaact gtccatattt
     7201 gccaagctcc tgtatttgct ctttactaca ggtaggcgtg gtagaacgtt aggtgaggaa
     7261 tatgttgact tgacatacac taataggaag ggtaccagac ttgcaggacg gctcaaaatg
     7321 attgtattcg ctttcgctta tcctttatgt ccatatttca tcaccaaact atacaagaaa
     7381 ataatgaaga acaacaagga aagtaaaatt gaagatactg aaagtgttgc cgcgttttgt
     7441 aagggccttt tagatttcat tttagacgta cacatgaccc tcttttattt caaaggagct
     7501 ttttacagta tttccaaaag gatttttggt atgagatatg tttttaaaca tatattgtca
     7561 aaaaatgagg ccaattttag agaagagggc tcacaaaagt acaaagtatt aggttatatc
     7621 ctattagctc aaaatgttat gaaatggtac cctgtcctaa cctctacgct cgggtcctgg
     7681 atttatggga gaaaaaggac caatgattca attactaggt cgtctgttgg tctacaggag
     7741 cgttcagaac atgaatctat agaaggcata ccgaaggaat cgcagctgac tcatataaat
     7801 ttgtcagata aaaaccaatt gccatttatt ccagaagcct cgagaaaatg catcctatgt
     7861 ctcatgaaca tgagtgatcc tagctgcgcg ccatgtggac atctattttg ttggagttgt
     7921 cttatgagct ggtgtaaaga gaggccagaa tgccccttgt gcagacaaca ctgtcaacca
     7981 caggaaattc tggtcctgcg gcaataggga tcctaactcg agagtgcttt taactaagaa
     8041 ttattagtct tttctgctta ttttttcatc atagtttaga acactttata ttaacgaata
     8101 gtttatgaat ctatttaggt ttaaaaattg atacagtttt ataagttact ttttcaaaga
     8161 ctcgtgctgt ctattgcata atgcactgga aggggaaaaa aaaggtgcac acgcgtggct
     8221 ttttcttgaa tttgcagttt gaaaaatgct gaagtgaata aagctccaca cagtcgaacg
     8281 actagagatc tatgtgagga tcctaactcg agatcgagct gagcagttac agagatgtta
     8341 cgaaccacta gtgcactgca gtacatgaca tgtatgggtt gaaaatattt agaggatgct
     8401 aaaagtcccg tctaaaatag aggaagtcat ggattacatt atcaccttta ttgtcctgtt
     8461 ccgtctggct aatttgggac gaggaacgtt tgtctccatt cgcaaccttt tgaaaccata
     8521 tgaacggcgg ctaatctgac agatgtctta tatttctagg attgtagaaa gcctcctcat
     8581 caaaccacct tgtttgaaaa aggcactagt gcggttgctt tacttgatct agtattctcc
     8641 taccttggag cggtcccata tcaactaaat tcagttttct gactgagtta aaaactaatg
     8701 tagcgacacc acttccattg gtgcctccgt tatttttttt ttgtatgtta atgctaaata
     8761 atacaccgct atgtatttca gggcactact tctacacatc aacggtacta aacatttcgc
     8821 tcaagatcgg tccgctttca cgtatttgga tgctatgcaa tgttgccgag cggccgcgtg
     8881 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     8941 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     9001 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     9061 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     9121 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     9181 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     9241 caaaccgtta ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa
     9301 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     9361 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     9421 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     9481 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     9541 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     9601 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     9661 atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat
     9721 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     9781 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
     9841 tcgaactttt gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg
     9901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10621 gtggataacc gtgcggccgc caattgaaaa aggccactac tgagccaagc gcagaaccag
    10681 atgagccatt cattggatac ttgggtgacg ttactgcatc tataaaccaa gatataaaag
    10741 aatatggtcg cagaagaaca gtaataccta caagcgattt agtagcgtcg ttctacacca
    10801 aaaagacgcc tgtttttaag gtcaagttca gcagaagcaa tttagccctt gcaggaggtg
    10861 ctttcaggcc ataattcaca ctgaaaaaaa taacagcatg gaagtgatat gtaataagta
    10921 taacctaatc cgatatgtaa ctattagatg ctttttcgac atcgtacttg acctgctcgg
    10981 cggttatcat agcgtcttct ttagcctttt ttttttcgct cagtcatcgg ggccaaaaca
    11041 agaaaggcga agaacagaaa aatgaaaaat gtaactggtt gttgtaaaaa ccttccagag
    11101 aaataagatg aaatgtatgt tgctggcacg aacgaagaca aataccctga attcgcatct
    11161 agactga
//
