LOCUS       sequence-431876-        7926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1503
FEATURES             Location/Qualifiers
     source          1..7926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2294..2481
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2349..2373)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2957..3364
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3365..4459
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5490..6305)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5490..6305)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6213..6232
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6297..6316
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6530..7118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7019..7038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatga gtggtaacac
      721 aactaacgtg catgagacta gagccaagtt tgcagagacg ctgcaaccga ggattggtgg
      781 caatactaca aaagtgatac gagctgcttt ggaaaagaac gaagctgaaa gcggggtgtc
      841 tgaggataat gataacgggt cattggagaa agttaacgta gctacttcgc cgcttttgac
      901 atcaacaccg cccacaatct caaaggcgtt ggtgaaattg tacccttatt taattctcat
      961 tgacgaattc ctaaacgttg ttacatggac aggaaagaat atttggagca gtgttttgat
     1021 gctgtgtctc tttattacta ccgttgagta ttttgaaacg ctggtgaagt actttggaca
     1081 tcttgccatc attgctattt tgtggggtta ttccctattg gacaattata ttgagggtac
     1141 gttgagctcc tcaccaacat tagaagatat tgctctctta atgaacagag tatccttgaa
     1201 gtcagatatt ttgctctccc ccatggttaa ccttggaacg caagatattc aacggcttct
     1261 atatactacg gtcatattat ctccaatata cgtgatgata acctggctac ttttaccacc
     1321 gagaagcttg atgctgatgg tgggcatgtt ccttttaacg tatcactcac catggtctaa
     1381 agtagcaaga aggcttttat ggaagtttaa gattgtcagg ctgcttgttt tctacgtcac
     1441 gggtttagac cttggtggaa taaataagga ccagggtatt tttgctacag tgcagaagca
     1501 agtgaaaaag ttggcatcaa cagaaaacag taatggcgta ttatccgatt ccaagcctat
     1561 ccgttttact tatgttttgt acgagaacca gcgtcgttgg ttaggtattg gatggaagcc
     1621 tagtatgttg agttatgaga ggactccatg gaccgatgaa tttttgaatg aggctccctc
     1681 tcctgaaaac ttccatctac ctgaagagac taacactatg gtttggagat gggtcgacaa
     1741 gacatggagg ctagacatga ctaatgacgg tgccattcaa gtgcccaatt caaaggcaag
     1801 aacttccgca gatccctccc ctgacgaggg atttatatat tacgacaata cctggaaaaa
     1861 accaagtaaa gaagattcct tttctaaata tacaagaaga agaagatggg taagaaccgc
     1921 agaattggtc aaaacttctg actttgatga aagcgtgata aattcgaata gaaattctgc
     1981 cattgaacaa aaggttgaag aaaacagcac gaatggttta accgctgagc aagagcttgg
     2041 gagcaacaag caagagaaag ataatgcgaa aaaagtagga gagcctacaa cagaagagac
     2101 aaaagaattt gccgaagcat caaacatcaa cgaaggcgag tttgaaagaa tttcctcgac
     2161 cgatgaagaa gttttaaaat ctagggcaag agatcgactg gcaaaagtat tagatgatac
     2221 tgaagagaaa gagcaatcaa atccaaccat tggtcgcgat agcaagaagg ccgtatgagg
     2281 atcctaactc gaggcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     2341 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     2401 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
     2461 tattgaccac acctctaccg gcatgccgag caaatgcctg caaatcgctc cccatttcgc
     2521 tggaaatctg ctcgtcagtg gtgctcacac tgacgaatca tgtacagatc ataccgatga
     2581 ctgcctggcg actcacaact aagcaagaca gccggaacca gcgccggcga acaccactgc
     2641 atatatggca tatcacaaca gtccacgtct caagcagtta cagagatgtt acgaaccact
     2701 agtgcactgc agtacatcga ggagaacttc tagtatatct acatacctaa tattattgcc
     2761 ttattaaaaa tggaatccca acaattacat caaaatccac attctcttca aaatcaattg
     2821 tcctgtactt ccttgttcat gtgtgttcaa aaacgttata tttataggat aattatactc
     2881 tatttctcaa caagtaattg gttgtttggc cgagcggtct aaggcgcctg attcaagaaa
     2941 tatcttgacc gcagttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat
     3001 ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca
     3061 cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat
     3121 acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt
     3181 tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac
     3241 aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct
     3301 aacttttctt accttttaca tttcagcaat atatatatat atatttcaag gatataccat
     3361 tctaatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat
     3421 cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt
     3481 cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc
     3541 agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg
     3601 tcctaaatgg ggaaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga
     3661 acttcaattg tacgccaact taagaccatg taactttgca tccgactctc ttttagactt
     3721 atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt
     3781 gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag
     3841 tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct
     3901 acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc
     3961 aagattatgg agaaaaactg tggaggaaac catcaagaac gaatttccta cattgaaggt
     4021 tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa
     4081 tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat
     4141 cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac
     4201 cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt
     4261 caaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc
     4321 tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac
     4381 tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt
     4441 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat
     4501 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag
     4561 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa
     4621 ggaatacagg taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac
     4681 tttaacatta atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa
     4741 tcatgaaact atgattccta atttatatat tggaggattt tctctaaaaa aaaaaaaata
     4801 caacaaataa aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct
     4861 tgaaccattt cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc
     4921 ttaacgacgt agtcgagagt actcgtatcg catgtcggtg cgacacgaaa ttacaaaatg
     4981 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
     5041 agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgtga
     5101 taaggaaaaa gaattgcact ttaacattaa tattgacaag gaggagggca ccacacaaaa
     5161 agttaggtgt aacagaaaat catgaaacta tgattcctaa tttatatatt ggaggatttt
     5221 ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa tgacctgacc atttgatgga
     5281 gtttaagtca ataccttctt gaaccatttc ccataatggt gaaagttccc tcaagaattt
     5341 tactctgtca gaaacggcct taacgacgta gtcgacctcc tcttcagtac taaatctacc
     5401 aataccaaat ctgatggaag aatgggctaa tgcatcatcc ttacccagcg ccgagcggcc
     5461 gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     5521 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     5581 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     5641 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     5701 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     5761 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     5821 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg
     5881 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     5941 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     6001 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     6061 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     6121 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     6181 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     6241 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     6301 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     6361 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
     6421 ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt
     6481 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6541 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6601 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6661 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6721 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6781 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6841 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6901 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6961 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7021 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7081 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7141 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7201 attctgtgga taaccgtgcg gccgccaata cccagaagaa cagtaaaata aagcaaggta
     7261 cgtgaaatta atatttttaa atggttctaa ccgatgccga agaactgcgc agtccggtta
     7321 taacgtctga catgtccttt tttgatttgg aatccaacca ctcaagtgac tctgttcatt
     7381 tactttgcga aaaatatacc cacaaattgc ccatcgaaag tgaatcgcaa accaccttca
     7441 gactggcacc gacaaagcaa agattataca gacagagtac tttatacgta ccgttaagtc
     7501 tcaagcaaag ggttttctta tttactgaac gggtaaagag tatctgggcc ggcttgccaa
     7561 gatgcaaacc gaataagtat ttcaaagttg catttgcctt agccgtcctg acaccattgg
     7621 ctatttggat attttatatt gactttcgtg tacattgatc acatcgactg ttctattggc
     7681 aaatgaacca cgggcattga ctatttttca ggttactact atatattatc atcacgggca
     7741 aggattgtac cctgaattcg catctagact gatgagacgt ggtagagcca caaacagccg
     7801 gtacaagcaa cgatctccag gaccatctga atcatgcgcg gatgacacga actcacgacg
     7861 gcgatcacag acattaaccc acagtacaga cactgcgaca acgtggcaat tcgtcgcaat
     7921 acaacg
//
