LOCUS       sequence-431880-        6954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1333
FEATURES             Location/Qualifiers
     source          1..6954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1322..1509
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1377..1401)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1985..2392
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2393..3487
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(4518..5333)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4518..5333)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5241..5260
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5325..5344
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5558..6146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6047..6066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatga catcgattaa
      721 cagttttccc aggaatattg actggccttc caacataggc attaaaaaaa tagagggaac
      781 taatccaacg gtgaatgcca tcaagggctt gttatataat ggtggatcaa tttatgcatt
      841 tctatatttt gttattgcta tgtttgttga accaacgcta caaaagcagt accagcagag
      901 aaatgatttt tccttgtttg ttttgttgcg tttgagaaga atcatagcgc aattgcaaaa
      961 acgactggtg atgaccccag tatcgtcgtt ggggttcaac gaacagaata actttgtgga
     1021 gaggtccact caaacttcag acgacaatat aatacgagaa gataatagcc attgggctga
     1081 aatgatttat caactgcaaa atatgaaaca agaattacag tattttaaca gatcctcagg
     1141 ccaaccatct gaaagtatag acgattttgt ctttcaaatt aagatggtga ctgaccaggt
     1201 tgagttaaca gataggtccc gagctttctc aaataaatca agaaatatta tacagggaat
     1261 ccgagaaatc aaaggttggt ttgtgaatgg ccaagtgcca aggtaaggat cctaactcga
     1321 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     1381 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     1441 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     1501 ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcgctg gaaatctgct
     1561 cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact gcctggcgac
     1621 tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat atatggcata
     1681 tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag tgcactgcag
     1741 tacatcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     1801 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     1861 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     1921 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     1981 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     2041 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     2101 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     2161 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     2221 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     2281 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     2341 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     2401 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     2461 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     2521 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     2581 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     2641 aaccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     2701 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     2761 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     2821 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     2881 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     2941 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     3001 aaaaactgtg gaggaaacca tcaagaacga atttcctaca ttgaaggttc aacatcaatt
     3061 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     3121 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     3181 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     3241 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     3301 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     3361 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     3421 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     3481 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     3541 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     3601 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     3661 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     3721 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     3781 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     3841 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     3901 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     3961 tcgagagtac tcgtatcgca tgtcggtgcg acacgaaatt acaaaatgga atatgttcat
     4021 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
     4081 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
     4141 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
     4201 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
     4261 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
     4321 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
     4381 aacggcctta acgacgtagt cgacctcctc ttcagtacta aatctaccaa taccaaatct
     4441 gatggaagaa tgggctaatg catcatcctt acccagcgcc gagcggccgc gtgttacaac
     4501 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     4561 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     4621 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     4681 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     4741 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     4801 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     4861 ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa
     4921 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     4981 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     5041 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     5101 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     5161 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     5221 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     5281 ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc
     5341 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     5401 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     5461 tttgctgagt tgaaggatca gtcatgacca aaatccctta acgtgagttt tcgttccact
     5521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5701 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6181 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6241 accgtgcggc cgccaatacc cagaagaaca gtaaaataaa gcaaggtacg tgaaattaat
     6301 atttttaaat ggttctaacc gatgccgaag aactgcgcag tccggttata acgtctgaca
     6361 tgtccttttt tgatttggaa tccaaccact caagtgactc tgttcattta ctttgcgaaa
     6421 aatataccca caaattgccc atcgaaagtg aatcgcaaac caccttcaga ctggcaccga
     6481 caaagcaaag attatacaga cagagtactt tatacgtacc gttaagtctc aagcaaaggg
     6541 ttttcttatt tactgaacgg gtaaagagta tctgggccgg cttgccaaga tgcaaaccga
     6601 ataagtattt caaagttgca tttgccttag ccgtcctgac accattggct atttggatat
     6661 tttatattga ctttcgtgta cattgatcac atcgactgtt ctattggcaa atgaaccacg
     6721 ggcattgact atttttcagg ttactactat atattatcat cacgggcaag gattgtaccc
     6781 tgaattcgca tctagactga tgagacgtgg tagagccaca aacagccggt acaagcaacg
     6841 atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc gatcacagac
     6901 attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac aacg
//
