LOCUS       sequence-431883-       10468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert 
            IPP to geraniol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1439
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3961..4627
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4497..4520
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      6291..6478
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6346..6370)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             6741..7544
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8186..9001)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8186..9001)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8909..8928
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8993..9012
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9226..9814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9715..9734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgtgtgg agtgtttgct tggattcttt agtaaaaggg
       61 gaagaacagt tggaagggcc aaagtggaag tcacaaaaca gtggtcctat ataaaagaac
      121 aagaaaaaga ttatttatat acaactgcgg tcacaagaag caacgcgaga gagcacaaca
      181 cgctgttatc acgcaaacta tgttttgaca ccgagccata gccgtgattg tgcgtcacat
      241 tgggcgataa tgaacgctaa atgaccaact cccatccgta ggagcccctt agggcgtgcc
      301 aatagtttca cgcgcttaat gcgaagtgct cggaacggac aactgtggtc gtttggcacc
      361 gggaaagtgg tactagaccg agagtttcgc atttgtatgg caggacgttc tgggagcttc
      421 gcgtctaaag ctttttcggg cgcgaaatgc agaccagacc agaacaaaac aactgacaag
      481 aaggcgttta atttaatatg ttgttcactc gcgcctgggc tgttgttatt cggctagata
      541 catacgtgtt tgtgcgtatg tagttatatc atatataagt atattaggat gaggcggtga
      601 aagagatttt ttttttttcg cttaatttat tcttttctct atcttttttc ctacatcttg
      661 ttcaaaagag tagcaaaaac aacaatcaat acaataaaat aagatctatg actgccgaca
      721 acaatagtat gccccatggt gcagtatcta gttacgccaa attagtgcaa aaccaaacac
      781 ctgaagacat tttggaagag tttcctgaaa ttattccatt acaacaaaga cctaataccc
      841 gatctagtga aacgtcaaat gacgaaagcg gagaaacatg tttttctggt catgatgagg
      901 agcaaattaa gttaatgaat gaaaattgta ttgttttgga ttgggacgat aatgctattg
      961 gtgccggtac caagaaagtt tgtcatttaa tggaaaatat tgaaaagggt ttactacatc
     1021 gtgcattctc cgtctttatt ttcaatgaac aaggtgaatt acttttacaa caaagagcca
     1081 ctgaaaaaat aactttccct gatctttgga ctaacacatg ctgctctcat ccactatgta
     1141 ttgatgacga attaggtttg aagggtaagc tagacgataa gattaagggc gctattactg
     1201 cggcggtgag aaaactagat catgaattag gtattccaga agatgaaact aagacaaggg
     1261 gtaagtttca ctttttaaac agaatccatt acatggcacc aagcaatgaa ccatggggtg
     1321 aacatgaaat tgattacatc ctattttata agatcaacgc taaagaaaac ttgactgtca
     1381 acccaaacgt caatgaagtt agagacttca aatgggtttc accaaatgat ttgaaaacta
     1441 tgtttgctga cccaagttac aagtttacgc cttggtttaa gattatttgc gagaattact
     1501 tattcaactg gtgggagcaa ttagatgacc tttctgaagt ggaaaatgac aggcaaattc
     1561 atagaatgct aggatccttg ggaagaggta gaagatccaa attgtaataa ctcgagtggc
     1621 attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc catcctttac
     1681 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga
     1741 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt
     1801 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgtgctgc caaaccagat
     1861 gtcaacacag ctacaacgca cccatgaacc acacggttag tccaaaaggg gcagttcaga
     1921 ttccagatgc gggaattagc ttgctgccac cctcacctca ctaacgctgc ggtgtgcgga
     1981 tacttcatgc tatttataga cgcgcgtgtc ggaatcagca cgcgcaagaa ccaaatggga
     2041 aaatcggaat gggtccagaa ctgctttgag tgctggctat tggcgtctga tttccgtttt
     2101 gggaatcctt tgccgcgcgc ccctctcaaa actccgcaca agtcccagaa agcgggaaag
     2161 aaataaaacg ccaccaaaaa aaaaaaaata aaagccaatc ctcgaagcgt gggtggtagg
     2221 ccctggatta tcccgtacaa gtatttctca ggagtaaaaa aaccgtttgt tttggaattt
     2281 cccatttcgc ggccacctac gccgctatct ttgcaacaac tatctgcgat aactcagcaa
     2341 attttgcata ttcgtgttgc agtattgcga taatgggagt cttacttcca acataacggc
     2401 agaaagaaat gtgagaaaat tttgcatcct ttgcctccgt tcaagtatat aaagtcggca
     2461 tgcttgataa tctttctttc catcctacat tgttctaatt attcttattc tcctttattc
     2521 tttcctaaca taccaagaaa ttaatcttct gtcattcgct taaacactat atcaataaag
     2581 atctatggct tcagaaaaag aaattaggag agagagattc ttgaacgttt tccctaaatt
     2641 agtagaggaa ttgaacgcat cgcttttggc ttacggtatg cctaaggaag catgtgactg
     2701 gtatgcccac tcattgaact acaacactcc aggcggtaag ctaaatagag gtttgtccgt
     2761 tgtggacacg tatgctattc tctccaacaa gaccgttgaa caattggggc aagaagaata
     2821 cgaaaaggtt gccattctag gttggtgcat tgagttgttg caggcttact ggttggtcgc
     2881 cgatgatatg atggacaagt ccattaccag aagaggccaa ccatgttggt acaaggttcc
     2941 tgaagttggg gaaattgcca tctgggacgc attcatgtta gaggctgcta tctacaagct
     3001 tttgaaatct cacttcagaa acgaaaaata ctacatagat atcaccgaat tgttccatga
     3061 ggtcaccttc caaaccgaat tgggccaatt gatggactta atcactgcac ctgaagacaa
     3121 agtcgacttg agtaagttct ccctaaagaa gcactccttc atagttactt tcaagactgc
     3181 ttactattct ttctacttgc ctgtcgcatt ggccatgtac gttgccggta tcacggatga
     3241 aaaggatttg aaacaagcca gagatgtctt gattccattg ggtgaatact tccaaattca
     3301 agatgactac ttagactgct tcggtacccc agaacagatc ggtaagatcg gtacagatat
     3361 ccaagataac aaatgttctt gggtaatcaa caaggcattg gaacttgctt ccgcagaaca
     3421 aagaaagact ttagacgaaa attacggtaa gaaggactca gtcgcagaag ccaaatgcaa
     3481 aaagattttc aatgacttga aaattgaaca gctataccac gaatatgaag agtctattgc
     3541 caaggatttg aaggccaaaa tttctcaggt cgatgagtct cgtggcttca aagctgatgt
     3601 cttaactgcg ttcttgaaca aagtttacaa gagaagcaaa ggatccgggt ccttgggaag
     3661 aggtagaaga tccaaattgt agtaatggcg ccaattggtg cggcaattga taataacgaa
     3721 aatgtctttt aatgatctgg gtataatgag gaattttccg aacgttttta ctttatatat
     3781 atatatacat gtaacatata ttctatacgc tatagagaaa ggaaattttt caattaaaaa
     3841 aaaaatagag aaagagtttc acttcttgat tatcgctaac actaatggtt gaagtactgc
     3901 tactttaatt ttatgctgga tgcacacact ggcttaagat gacaacgcag ttcgagttta
     3961 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
     4021 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
     4081 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
     4141 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
     4201 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
     4261 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
     4321 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
     4381 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
     4441 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
     4501 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
     4561 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
     4621 aaacaaaaga tctatgatcg ctgccccatt agttagagat aacggttcag cattgaactt
     4681 cttcccacaa gccccacaag tagaaatcga tgagtcatca agaataatgg agttagttga
     4741 agcaaccagg agaactttaa gaaatgaatc atctgattct actgagaaaa tgagattgat
     4801 cgactctttg caaagattag gtttgaacca tcactttgaa caagatataa aagaaatgtt
     4861 acaggatttt gcaaacgaac acaagaatac caatcaagat ttgttcacta catctttgag
     4921 atttaggttg ttaagacaca atggtttcaa tgtaacacca gacgtcttca ataaatttac
     4981 cgaggaaaat ggtaagttca aggaatcatt aggtgaggat acaatcggaa tattgtcttt
     5041 atacgaagcc tcttatttag gaggtaaagg tgaagagatt ttatctgagg ctatgaagtt
     5101 ttctgaatct aaattgaggg aatcttctgg acacgtagca ccacatatca gaaggcaaat
     5161 atttcagtca ttggaattgc caaggcattt gaggatggct agattagaat caagaaggta
     5221 catcgaagaa gactactcta acgaaatcgg agccgattca tcattattag agttagctaa
     5281 attggatttc aattctgttc aagcattgca tcaaatggaa ttaactgaga ttagtagatg
     5341 gtggaagcag ttaggtttgt ctgataaatt acctttcgca agagacagac ctttggagtg
     5401 cttcttatgg actgtaggat tattaccaga accaaaatac tcaggttgca gaatcgagtt
     5461 ggccaagact attgctgttt tgttggttat tgacgacatc tttgacacct atggatctta
     5521 tgatcagttg atattattta caaacgctat aagacgttgg gatttagacg ccatggacga
     5581 gttgcctgaa tatatgaaaa tctgttacat ggcattatac aacactacaa atgagatatg
     5641 ttataaagtt ttgaaagaaa atggatggtc tgtattacct tacttagaga gaacctggat
     5701 tgacatggtt gagggtttta tgttagaggc taaatggtta aactcaggtg agcaaccaaa
     5761 tttagaagct tatatagaaa acggtgtaac cacagctgga tcatacatgg ctcttgttca
     5821 tctcttcttt cttataggtg acggagttaa tgatgaaaac gtaaaattgt tattggaccc
     5881 atatccaaag ttattctcat ctgccggtag aattttgaga ttatgggacg atttaggaac
     5941 tgcaaaggaa gagcaagaaa ggggtgacgt atcatcttca atccaattat acatgaagga
     6001 gaagaatgtt aggtcagaat ctgaaggaag ggagggtata gtcgagatta tttacaattt
     6061 gtggaaagat atgaatggtg agttaattgg ttctaatgct ttgccacaag ccattattga
     6121 gacatctttt aacatggcga gaacttctca agtagtctac caacacgagg atgacacata
     6181 cttttcttct gttgataatt acgtccaatc attgttcttc acaccagtat ctgtttctgt
     6241 tggatccggg tccttgggaa gaggtagaag atccaaattg tagtaatggc gcgaatttct
     6301 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     6361 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     6421 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca
     6481 tgccgagcaa atgcctgcaa atcgctcccc atttcgctga gcagttacag agatgttacg
     6541 aaccactagt gcactgcagt acacggtttc cttgaaattt ttttgattcg gtaatctccg
     6601 aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta
     6661 gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacgt
     6721 gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc
     6781 ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg
     6841 cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca
     6901 aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag
     6961 ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat
     7021 ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag
     7081 aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga
     7141 agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt
     7201 catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga
     7261 gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag
     7321 gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga gatgcattgg
     7381 gtcaacagta tagaaccgtg gatgatgtgg tttctacagg atctgacatt attattgttg
     7441 gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag
     7501 caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact gtattataag
     7561 taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
     7621 ccgagtagag cacttgaatc cactgccccg ggaatctcgg tcgtaatgat ttctataatg
     7681 acgaaaaaaa aaaaattgga aagaaaaagc ttcatggcct ttataaaaag gaactatcca
     7741 atacctcgcc agaaccaagt aacagtattt tacggggcac aaatcaagaa caataagaca
     7801 ggactgtaaa gatggacgca ttgaactcca aagaacaaca agagttccaa aaagtagtgg
     7861 aacaaaagca aatgaaggat ttcatgcgtt tgtactctaa tctggtagaa agatgtttca
     7921 cagactgtgt caatgacttc acaacatcaa agctaaccaa taaggaacaa acatgcatca
     7981 tgaagtgctc agaaaagttc ttgaagcata gcgaacgtgt agggcagcgt ttccaagaac
     8041 aaaacgctgc cttgggacaa ggcttgggcc gataaggtgt actggcgtat atatatctaa
     8101 ttatgtatct ctggtgtagc ccatttttag catgtaaata taaagaccga gcggccgcgt
     8161 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     8221 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     8281 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     8341 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8401 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt
     8461 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8521 ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa
     8581 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8641 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     8701 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     8761 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     8821 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     8881 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     8941 catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga atatggctca
     9001 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     9061 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
     9121 atcgaacttt tgctgagttg aaggatcagt catgaccaaa atcccttaac gtgagttttc
     9181 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9241 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9301 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9361 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9421 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9481 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9541 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9601 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9661 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9721 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9781 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9841 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9901 tgtggataac cgtgcggccg ccaatgctaa attcgagtga aacacaggaa gatcagaaaa
     9961 tcctcatttc atccatatta acaataattt caaatgttta tttgcattat ttgaaactag
    10021 gcaagacaag caacgaaacg tttttgaaaa ttttgagtat tttcaataaa tttgtagagg
    10081 actcagatat tgaaaaaaag ctacagcaat taatacttga taagaagagt attgagaagg
    10141 gcaacggttc atcatctcat ggatctgcac atgaacaaac accagagtca aacgacgttg
    10201 aaattgaggc tactgcgcca attgatgaca atacagacga tgataacaaa ccgaagttat
    10261 ctgatgtaga aaaggattaa agatgctaag agatagtgat gatatttcat aaataatgta
    10321 attctatata tgttaattac cttttttgcg aggcatattt atggtgaagg ataagttttg
    10381 accatcaaag aaggttaatg tggctgtggt ttcagggtcc ataaagccca catggataac
    10441 attacccctg aattcgcatc tagactga
//
