LOCUS       sequence-431884-       10660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pKS1469
FEATURES             Location/Qualifiers
     source          1..10660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        39..705
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     575..598
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      1910..2097
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1965..1989)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2441..2854
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        5845..6252
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6253..7347
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(8378..9193)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8378..9193)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9101..9120
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9185..9204
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9418..10006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9907..9926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgcagtt cgagtttatc attatcaata ctgccatttc
       61 aaagaatacg taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta
      121 gccttttaat tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata
      181 tataacatcg taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga
      241 gcccgctttt taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt
      301 cttcaccaac catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca
      361 caaacaggca aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg
      421 atgacacaag gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac
      481 cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa
      541 ttattcccct acttgactaa taagtatata aagacggtag gtattgattg taattctgta
      601 aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa
      661 aacaccaaga acttagtttc gaataaacac acataaacaa acaaaagatc tatgacaatt
      721 gggattgata aaattagttt ttttgtgccc ccttattata ttgatatgac ggcactggct
      781 gaagccagaa atgtagaccc tggaaaattt catattggta ttgggcaaga ccaaatggcg
      841 gtgaacccaa tcagccaaga tattgtgaca tttgcagcca atgccgcaga agcgatcttg
      901 accaaagaag ataaagaggc cattgatatg gtgattgtcg ggactgagtc cagtatcgat
      961 gagtcaaaag cggccgcagt tgtcttacat cgtttaatgg ggattcaacc tttcgctcgc
     1021 tctttcgaaa tcaaggaagg ttgttacgga gcaacagcag gcttacagtt agctaagaat
     1081 cacgtagcct tacatccaga taaaaaagtc ttggtcgtag cggcagatat tgcaaaatat
     1141 ggcttaaatt ctggcggtga gcctacacaa ggagctgggg cggttgcaat gttagttgct
     1201 agtgaaccgc gcattttggc tttaaaagag gataatgtga tgctgacgca agatatctat
     1261 gacttttggc gtccaacagg ccacccgtat cctatggtcg atggtccttt gtcaaacgaa
     1321 acctacatcc aatcttttgc ccaagtctgg gatgaacata aaaaacgaac cggtcttgat
     1381 tttgcagatt atgatgcttt agcgttccat attccttaca caaaaatggg caaaaaagcc
     1441 ttattagcaa aaatctccga ccaaactgaa gcagaacagg aacgaatttt agcccgttat
     1501 gaagaaagta tcgtctatag tcgtcgcgta ggaaacttgt atacgggttc actttatctg
     1561 ggactcattt cccttttaga aaatgcaacg actttaaccg caggcaatca aattggttta
     1621 ttcagttatg gttctggtgc tgtcgctgaa tttttcactg gtgaattagt agctggttat
     1681 caaaatcatt tacaaaaaga aactcattta gcactgctgg ataatcggac agaactttct
     1741 atcgctgaat atgaagccat gtttgcagaa actttagaca cagacattga tcaaacgtta
     1801 gaagatgaat taaaatatag tatttctgct attaataata ccgttcgttc ttatcgaaac
     1861 ggatccttgg gaagaggtag aagatccaaa ttgtaataac tcgagtggcg cgaatttctt
     1921 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     1981 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     2041 caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat
     2101 gccgagcaaa tgcctgcaaa tcgctcccca tttcgctgcc aaaccagatg tcaacacagc
     2161 tacaacgcct tgccaacagg gagttcttca gagacatgga ggctcaaaac gaaattattg
     2221 acagcctaga catcaatagt catacaacag aaagcgacca cccaactttg gctgataata
     2281 gcgtataaac aatgcatact ttgtacgttc aaaatacaat gcagtagata tatttatgca
     2341 tattacatat aatacatatc acataggaag caacaggcgc gttggacttt taattttcga
     2401 ggaccgcgaa tccttacatc acacccaatc ccccacaagt gatcccccac acaccatagc
     2461 ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc
     2521 gtaccacttc aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg
     2581 tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agacaccgcc tcgtttcttt
     2641 ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaaatttttt
     2701 tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtcat
     2761 caatttctca agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc
     2821 tcattagaaa gaaagcatag caatctaatc taagttttaa ttacaaaaga tctatgaaga
     2881 ctgtcgttat catagatgcc ttgagaacac caatcggtaa atacaaaggt tcattatccc
     2941 aagtttccgc cgttgactta ggtactcatg ttactacaca attgttgaag agacactcca
     3001 caatcagtga agaaatcgat caagtcatat tcggtaacgt attgcaagct ggtaatggtc
     3061 aaaacccagc cagacaaata gctatcaatt ctggtttatc acatgaaatt cctgctatga
     3121 cagtaaacga agtttgtggt tcaggcatga aagcagtcat tttggccaag caattgatac
     3181 aattaggtga agcagaagtt ttaatcgccg gtggtataga aaacatgagt caagctccaa
     3241 aattgcaaag attcaattac gaaactgaat cttacgatgc acctttctct tcgatgatgt
     3301 atgatggttt gactgacgct ttttctggtc aagcaatggg tttaacagct gaaaatgtcg
     3361 cagaaaagta ccatgtaacc agagaagaac aagatcaatt ttccgttcac agtcaattaa
     3421 aagctgcaca agcacaagcc gaaggtattt tcgccgacga aatagctcca ttggaagttt
     3481 ctggtacatt agtcgaaaag gatgaaggta ttagacctaa ctccagtgtt gaaaaattgg
     3541 gtactttgaa gacagtattc aaggaagacg gtacagttac cgctggtaat gcctctacca
     3601 ttaacgatgg tgctagtgca ttgattatag cttctcaaga atatgccgaa gctcatggtt
     3661 tgccatactt agctatcatt agagatagtg tagaagttgg tattgaccca gcatacatgg
     3721 gtatctctcc tataaaagca atccaaaagt tgttagccag aaaccaattg accactgaag
     3781 aaattgattt gtacgaaatt aacgaagcat ttgccgctac atcaatcgtt gtccaaagag
     3841 aattggcatt gccagaagaa aaggttaaca tctatggtgg tggtatctcc ttgggtcacg
     3901 ctataggtgc aaccggtgcc agattgttga cttccttaag ttaccaattg aaccaaaagg
     3961 aaaagaaata cggtgttgct tctttatgca ttggtggtgg tttgggttta gcaatgttgt
     4021 tagaaagacc acaacaaaag aaaaattcta gattctacca aatgtcccct gaagaaagat
     4081 tggcctcatt gttaaatgaa ggtcaaattt ccgcagatac taagaaagaa tttgaaaaca
     4141 ccgctttatc ttcacaaatc gcaaaccata tgatcgaaaa ccaaatctct gaaacagaag
     4201 ttccaatggg tgtcggtttg cacttaactg tcgatgaaac agactatttg gtaccaatgg
     4261 ctaccgaaga acctagtgtt atcgcagcct tatctaatgg tgctaagata gcacaaggtt
     4321 ttaagactgt taaccaacaa agattgatga gaggtcaaat cgtattctac gatgttgctg
     4381 acccagaatc attaatcgat aagttgcaag taagagaagc cgaagttttt caacaagctg
     4441 aattgtctta cccttcaata gttaagagag gtggtggttt gagagatttg caatacagaa
     4501 cttttgacga atccttcgtc agtgtagatt tcttagttga tgtcaaggac gccatgggtg
     4561 ctaatattgt taacgcaatg ttggaaggtg tcgccgaatt gtttagagaa tggttcgctg
     4621 aacaaaagat tttgttttct atcttgtcaa actacgctac agaatctgta gttaccatga
     4681 aaactgcaat tccagtttcc agattgagta agggttctaa cggtagagaa atcgctgaaa
     4741 agattgtttt ggcatcaaga tatgcctcct tagaccctta cagagctgtt actcataata
     4801 agggtataat gaacggtatc gaagctgtcg tattagcaac cggtaatgat actagagcag
     4861 tatctgcctc atgtcacgca ttcgccgtta aggaaggtag ataccaaggt ttgacatcat
     4921 ggaccttgga tggtgaacaa ttaattggtg aaatatccgt tccattggct ttagcaactg
     4981 ttggtggtgc tacaaaagtc ttgcctaaga gtcaagctgc agccgatttg ttagccgtca
     5041 ctgacgctaa ggaattgtct agagttgtcg ctgcagtagg tttagctcaa aatttggccg
     5101 ctttaagagc attggtttca gaaggtattc aaaaaggtca tatggctttg caagcaagat
     5161 ccttagccat gacagttggt gctaccggta aagaagtcga agccgtagct caacaattaa
     5221 aaagacaaaa gacaatgaac caagacagag caatggctat attaaacgat ttgagaaagc
     5281 aaggatcctt gggaagaggt agaagatcca aattgtaata actcgagtgg cattgaattg
     5341 aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta cgctaaaata
     5401 atagtttatt ttattttttg aatatttttt atttatatac gtatatatag actattattt
     5461 atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt ttaatgcctt
     5521 tatgcagttt ttttttccca ttcgatattt ctatgtgctg agcagttaca gagatgttac
     5581 gaaccactag tgcactgcag tacatcgagg agaacttcta gtatatctac atacctaata
     5641 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa
     5701 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa
     5761 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat
     5821 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga
     5881 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg
     5941 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg
     6001 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa
     6061 ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc acaatacttg
     6121 aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta gcaatcgtct
     6181 tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat atttcaagga
     6241 tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
     6301 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat
     6361 gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt
     6421 ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt gttaggtgct
     6481 gtgggtggtc ctaaatgggg aaccggtagt gttagacctg aacaaggttt actaaaaatc
     6541 cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt
     6601 ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga
     6661 gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct
     6721 tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc
     6781 atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg
     6841 gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga atttcctaca
     6901 ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc
     6961 cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
     7021 tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac
     7081 aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag
     7141 aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg
     7201 aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt
     7261 atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
     7321 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     7381 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     7441 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     7501 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
     7561 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
     7621 acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa
     7681 aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa
     7741 taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag
     7801 aaacggcctt aacgacgtag tcgagagtac tcgtatcgca tgtcggtgcg acacgaaatt
     7861 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
     7921 caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct
     7981 caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcacc
     8041 acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt tatatattgg
     8101 aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat
     8161 ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc
     8221 aagaatttta ctctgtcaga aacggcctta acgacgtagt cgacctcctc ttcagtacta
     8281 aatctaccaa taccaaatct gatggaagaa tgggctaatg catcatcctt acccagcgcc
     8341 gagcggccgc gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
     8401 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     8461 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     8521 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     8581 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     8641 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     8701 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc
     8761 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     8821 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     8881 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     8941 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     9001 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     9061 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     9121 catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac gtttcccgtt
     9181 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     9241 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     9301 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gtcatgacca aaatccctta
     9361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10081 atcccctgat tctgtggata accgtgcggc cgccaatacc cagaagaaca gtaaaataaa
    10141 gcaaggtacg tgaaattaat atttttaaat ggttctaacc gatgccgaag aactgcgcag
    10201 tccggttata acgtctgaca tgtccttttt tgatttggaa tccaaccact caagtgactc
    10261 tgttcattta ctttgcgaaa aatataccca caaattgccc atcgaaagtg aatcgcaaac
    10321 caccttcaga ctggcaccga caaagcaaag attatacaga cagagtactt tatacgtacc
    10381 gttaagtctc aagcaaaggg ttttcttatt tactgaacgg gtaaagagta tctgggccgg
    10441 cttgccaaga tgcaaaccga ataagtattt caaagttgca tttgccttag ccgtcctgac
    10501 accattggct atttggatat tttatattga ctttcgtgta cattgatcac atcgactgtt
    10561 ctattggcaa atgaaccacg ggcattgact atttttcagg ttactactat atattatcat
    10621 cacgggcaag gattgtaccc tgaattcgca tctagactga
//
