LOCUS       sequence-431887-       16787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16787)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 16787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1401
FEATURES             Location/Qualifiers
     source          1..16787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1908..2095
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1963..1987)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        11972..12379
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12380..13474
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(14505..15320)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(14505..15320)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     15228..15247
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     15312..15331
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      15545..16133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16034..16053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgtttga gaatgacctt ccacgagcta aattgaaagg
       61 gaagaattta ttagttgaac tcaagaaaga agaggatgac gtgggaaatg gcatagaatc
      121 ccttactaaa tcgaacacta aactgaactc catgctggcg aacgaaggta agatacacaa
      181 agctagtttc cagaaaagtg taaaatttaa actacctgat aatatagtga ctgaagaaac
      241 cgtggaactt aaagaaataa aggacttgct actacaaatg ttgagatgac agcgagagat
      301 tgaatcaaga ttatccaata tcgaacttca actcacggaa ataccgaaac ataagtaatc
      361 atatcccttc tcacattttt tacacaggaa gtaagcaagt tatgttatat ttccgacact
      421 ataattaatt cttagcagtt aaaggtgctt tgtctatatt acatttacat acagcttgag
      481 tgatcctgac cggatatagg gtcctatttt cttacgtgaa cggcttttct tcttgttccc
      541 gatggccttc atgtgaaaaa gcactcctcg ggaggcggaa aaatatcaaa agtacggggc
      601 gaagtttata atgaagattt atcgatataa attttggtta tttcaggaga acaagaaagc
      661 tctttacact aaaattatca gagaagaagc tgatatatta gccctaagga gtacaccaaa
      721 acacaagatc tatgtcatcc acagcagtac cacgacctaa accttgggaa acaagtgctt
      781 cgttagagga gccacagcgt aatgctcagt ctttaagtgc gatgatgacc tcgaatcagc
      841 aggattccag gccaactgaa gaaagcaata acagtaatag tgcaagtgag tctgctcccg
      901 aagttttgcc gcggcctgcg gcacttaact cctctggtac ctacggcgaa tcgaatacaa
      961 taccgggaat atacggtaat agcaattatg ggatacccta tgataataat ccgtatagta
     1021 tgaactctat atacggaaac agcataggga ggtatggcta tggaggatcg tactatggca
     1081 acaattatgg atcgttttat ggaggagggt acggtgcagg agcgggatat ggaatgaata
     1141 atggaagtgg actaggtgag tcgacgaagg ccacctttca gttaatagaa agtttgatag
     1201 gcgctgttac tggatttgca caaatgctgg aatctactta tatggccaca cacaactcgt
     1261 ttttcaccat gatatccgtc gcagaacagt ttggtaattt gaaggaaatg ctaggttcat
     1321 tttttggtat ctttgctata atgaagtttc ttaaaaaaat actctatcgt gctactaagg
     1381 ggagactagg aataccacca aagaatttcg cagaatctga aggaagcaaa aataaactaa
     1441 ttgaagattt tcaaaagttt aatgatagtg gtaccataaa ttctaatgag aaagccacaa
     1501 gaaggaaaat ttcatggaaa ccgctactat tttttttgat ggcagtattt ggttttccat
     1561 atctactaaa caaatttatt actaaactac agacctctgg aaccatacga gcatcgcaag
     1621 gaaatggtag cgagcctatt gatccttcga agttagaatt tgcaagagcg ttatatgatt
     1681 ttgttccaga aaacccagag atggaagttg ctttaaaaaa gggcgacttg atggcaattt
     1741 tgagtaagaa agaccctctt gggagggatt ctgactggtg gaaagtgagg acaaagaacg
     1801 gcaatattgg ctacattccg tataactata ttgagatcat aaaaagacgg aagaaaattg
     1861 agcatgttga tgatgaaacg cgtacacact agggatccta actcgaggcg aatttcttat
     1921 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1981 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     2041 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     2101 cgagcaaatg cctgcaaatc gctccccatt tcgctgccaa accagatgtc aacacagcta
     2161 caacgtaagg gcatgataga attggattat gtaaaaggtg aagataccat tgtagaagca
     2221 accagcacgt cgccgtggct gatgaagtct cctcttgccc gggccgcaga aaagaggggc
     2281 agtggcctgt ttttcgacat aaatgagggg catggccagc accaagacgt cattgttgca
     2341 tatggcgtat ccaagccgaa acggcgctcg cctcatcccc acgggaataa ggcagccgac
     2401 aaaagaaaaa cgaccgaaaa ggaaccagaa agaaaaaaga gggtgggcgc gccgcggacg
     2461 tgtaaaaaga tatgcatcca gcttctatat cgctttaact ttaccgtttt gggcatcggg
     2521 aacgtatgta acattgatct cctcttggga acggtgagtg caacgaatgc gatatagcac
     2581 cgaccatgtg ggcaaattcg taataaattc ggggtgaggg ggattcaaga caagcaacct
     2641 tgttagtcag ctcaaacagc gatttaacgg ttgagtaaca catcaaaaca ccgttcgagg
     2701 tcaagcctgg cgtgtttaac aagttcttga tatcatatat aaatgtaata agaagtttgg
     2761 taatattcaa ttcgaagtgt tcagtctttt acttctcttg ttttatagaa gaaaaaacat
     2821 caagaaacat ctttaacata cacaaacaca tactatcaga atacaagatc tatgagtgac
     2881 gtggtcagta aagatcgtaa ggcattgttc gactcagcag tatccttttt aaaggatgag
     2941 tccattaaag atgctccact tttaaagaaa atcgaatttt taaaatccaa agggttaaca
     3001 gaaaaggaga ttgaaatagc catgaaagag cccaagaaag acggtatcgt aggcgatgaa
     3061 gtatcgaaaa aaattggtag tactgagaat agagcctcac aggatatgta tctctatgaa
     3121 gcgatgccac caacgctgcc ccacagggat tggaaggact attttgtgat ggctactgcc
     3181 acagctgggc tgttgtatgg tgcatatgaa gtaactagaa ggtatgtgat accaaatatt
     3241 ttaccagaag caaaaagcaa gttggaaggg gacaaaaaag aaattgatga tcagttctcc
     3301 aaaatcgata cagtcctcaa tgccatcgaa gcggagcaag ctgagtttag gaaaaaggaa
     3361 agcgaaacat taaaggaact tagtgacacg attgctgaac tgaaacaggc gcttgtgcag
     3421 acaacaagaa gcagggaaaa gatcgaggat gagtttagaa tagttaaact cgaggtggtc
     3481 aatatgcaaa atacgatcga caaatttgtt tcagataatg acggcatgca agagttaaat
     3541 aatatccaaa aagaaatgga atctctgaaa agcttaatga ataaccgtat ggaatccggt
     3601 aatgcgcagg acaacagatt attttccata tctcctaatg gtatacctgg catagatacg
     3661 attccatctg cgtctgagat tcttgccaaa atgggcatgc aagaagaaag tgataaagaa
     3721 aaggaaaacg gcagcgatgc taataaagat gacaatgctg ttccagcgtg gaaaaaagca
     3781 agagaacaaa ctattgatag caacgcctcc attccagaat ggcaaaaaaa taccgccgcc
     3841 aatgagatca gtgtccctga ctggcaaaat ggacaggtcg aggattccat cccataggga
     3901 tcctaactcg aggtgaattt actttaaatc ttgcatttaa ataaattttc tttttatagc
     3961 tttatgactt agtttcaatt tatatactat tttaatgaca ttttcgattc attgattgaa
     4021 agctttgtgt tttttcttga tgcgctattg cattgttctt gtctttttcg ccacatgtaa
     4081 tatctgtagt agatacctga tacattgtgg atgctgagtg aaattttagt taataatgct
     4141 ggatgcacac actggcttaa gatgacaacg ggatatcgta aacaaaggcg ttaccataga
     4201 aatgtactga ttggcagaat tactcttcag gagaatcttt catacaaagg tattccattg
     4261 gggaaaatct cgttaccaag tcaatgctga actttctatg gcctttgttt actatcgtta
     4321 attattttac gaccacttct gggtagaaat atttcgtagc cctggaacga gcttgtttac
     4381 gcgttttatc ccattatatg gcacccaaat caaatttaaa aagaaaaaac gcgtaaacag
     4441 tgtcgggtaa gttcatcctc tgttacttta attgcttctt tttttgaaat tctaagtaaa
     4501 cgcgtcattt tgatcctcag gacacagaaa tccttgcaga atcttattgg gtgttgaata
     4561 gaggacgcgt aaaaacgata tggaaatttt tttcatatag tgtagaaaga ataggttggc
     4621 gtaggtagtt tcgtgtttga tagaaacctc caacaaagtc tgcaactcac gttttagaat
     4681 aacaagttta gagtttatct tgttgccttt gttaagtcag taccattgaa taaaaattat
     4741 ataaaggagc taatatttca ttgttggaaa attactctac cataattgaa gcatatctca
     4801 tccttttcat ccttttcaac gcaagagaga caccaacgaa caacacttta tttgttgata
     4861 tattaacatc agatctatga catcgattaa cagttttccc aggaatattg actggccttc
     4921 caacataggc attaaaaaaa tagagggaac taatccaacg gtgaatgcca tcaagggctt
     4981 gttatataat ggtggatcaa tttatgcatt tctatatttt gttattgcta tgtttgttga
     5041 accaacgcta caaaagcagt accagcagag aaatgatttt tccttgtttg ttttgttgcg
     5101 tttgagaaga atcatagcgc aattgcaaaa acgactggtg atgaccccag tatcgtcgtt
     5161 ggggttcaac gaacagaata actttgtgga gaggtccact caaacttcag acgacaatat
     5221 aatacgagaa gataatagcc attgggctga aatgatttat caactgcaaa atatgaaaca
     5281 agaattacag tattttaaca gatcctcagg ccaaccatct gaaagtatag acgattttgt
     5341 ctttcaaatt aagatggtga ctgaccaggt tgagttaaca gataggtccc gagctttctc
     5401 aaataaatca agaaatatta tacagggaat ccgagaaatc aaaggttggt ttgtgaatgg
     5461 ccaagtgcca aggtaaggat cctaactcga gattgaattg aattgaaatc gatagatcaa
     5521 tttttttctt ttctctttcc ccatccttta cgctaaaata atagtttatt ttattttttg
     5581 aatatttttt atttatatac gtatatatag actattattt atcttttaat gattattaag
     5641 atttttatta aaaaaaaatt cgctcctctt ttaatgcctt tatgcagttt ttttttccca
     5701 ttcgatattt ctatgtgctg gttctgtatg ggcacagaca acctaaacga gtcgaacaag
     5761 aagcaggcaa agtttagagc actgcccctc cgcactcaaa aaagaaaaaa ctaggaggaa
     5821 aataaaattc tcaaccacac aaacacataa acacatacaa atacaaatac aagcttattt
     5881 acttgacatc gcgcgatctt ccactattca gcgccgtccg ccctctctcg tgttttttgt
     5941 ttacgcgaca actatgcgaa atccggagca acgggcaacc gtttggggaa agaccacacc
     6001 cacgcgcgat cgccatggca acgaggtcgc acacgcccca cacccagacc tccctgcgag
     6061 cgggcatggg tacaatgtcc ccgttgccac agacaccact tcgtagcaca gcgcagagcg
     6121 tagcgtgttg ttgctgctga caaaagaaaa tttttcttag caaagcaaag gaggggaagc
     6181 acgggcagat agcaccgtac catacccttg gaaactcgaa atgaacgaag caggaaatga
     6241 gagaatgaga gttttgtagg tatatatagc ggtagtgttt gcgcgttacc atcatcttct
     6301 ggatctatct attgttcttt tcctcatcac tttccccttt ttcgctcttc ttcttgtctt
     6361 ttatttcttt ctttttttta attgttccct cgattggcta tctaccaaag aatccaaact
     6421 taatacacgt atttatttgt ccaattacca gatctatgtc tcgagttgca cagctcgact
     6481 cgatagcgct tgataaagaa ttgtatggac agttttggtc cgagttcaat gcagcattca
     6541 atacgagtga acacaaagag gaatgggagt tggcactgaa cacagttgtg tttatgtgcg
     6601 cgacgaggtt cctgccacac tatggctcta gctgtacgta tggatccgca ctcagtggcg
     6661 tggtttttca gtgcaggaaa cgcaccttgt atgtcgtcac tgtcttggca gggtacgtgt
     6721 ggaagaagat cacacatatt atttttaatg ggccgcactg tgggaaccag atgatgtggc
     6781 tgaagctgta caaatgggtt aatttattgt atcacggctg tgatgtaacc aacttcctga
     6841 ggtttctagc agcggaaggg cccaatgcga gggcgttttt gtcgccgtta taccgggctt
     6901 ttaatgtcca ttccacaagg ttgattcggg atggttctgc aattgcctct gaattttatt
     6961 cgaactctgt attcgctggg ttagaatacc agaatagaca attgctatgg aatgcactat
     7021 tagaactctt ttccaataca ctacttacga aaagaggact cttgaccttt gttaaaaaac
     7081 caccaagatc tcgttctacg actacttata agacagtttg cccccgttgc ggcggttttc
     7141 caaccaaccc ataccagatc gcttgttgtc gtgcgaacta ctgttacgtg tgcgttgtaa
     7201 aggcgttgga gtggtctatg tgcgatgcct gtggatcctc tgggagactg accgcctcac
     7261 cagtgtacta aggatcctaa ctcgagggag attgataaga cttttctagt tgcatatctt
     7321 ttatatttaa atcttatcta ttagttaatt ttttgtaatt tatccttata tatagtctgg
     7381 ttattctaaa atatcatttc agtatctaaa aattcccctc ttttttcagt tatatcttaa
     7441 caggcgacag tccaaatgtt gatttatccc agtccgattc atcagggttg tgaagcattt
     7501 tgtcaatggt cgctgggtag actacccatg agtcacaatg aacgacgatg gcttcttatc
     7561 tcacttcaat agtactttcc accggttata cttccggctt ttccctatta atacaagcta
     7621 caatttcaat gggtggcaaa taatgtgtag aatagaaaat aagccgacag ggtaataaag
     7681 aaaattttta gaaaaaaaag gttagatggc ttatttaagt tacaggctag cgaaaaaagg
     7741 aacttcaggg caagtaaagt gtttgattgg gcactagcat ggcttataaa ggcgagcaat
     7801 tgtcgaaact aattaatgtt gtacggacta ttgctgtcat ctcgtggtaa atgcgtgttc
     7861 caggtcgaat actacttgca cacaggcgag cggggcccca taaaagtgtt gccgatttgt
     7921 taagttgtct tttcggtttt tctactctgt tattccttac ttcccttttt aagaactctt
     7981 tttatccttc atttaggatc ttgcacgttt ccgcctcatc acttgaatta aaacatgtct
     8041 ctgtcagtaa accttggcgt ttctattgtt cttcatagtt caacttttat tattacccgc
     8101 cctgcgcgtt tacatttttc cagcaacagc cagcgaaaaa ttagaaaatc tggttgttga
     8161 cacctcaaga acaagggcaa ttagcctcag cgtcgaatat agatcatatt agaataccta
     8221 tagctccatc aaaagaaata cacaagatct atgaagaatg ataataagtt gcaaaaggaa
     8281 gcacttatgc gactatcgca gctaagattt ccatttgccg atgctccaag tatagtacaa
     8341 gcgcatcaaa aagatgaaca aatccagggc cttttgatta tgaaagtgac tgaattgtgc
     8401 aaattgataa agagtcaatt gtttgttaac tcttacccta aggaactgtc catatttgcc
     8461 aagctcctgt atttgctctt tactacaggt aggcgtggta gaacgttagg tgaggaatat
     8521 gttgacttga catacactaa taggaagggt accagacttg caggacggct caaaatgatt
     8581 gtattcgctt tcgcttatcc tttatgtcca tatttcatca ccaaactata caagaaaata
     8641 atgaagaaca acaaggaaag taaaattgaa gatactgaaa gtgttgccgc gttttgtaag
     8701 ggccttttag atttcatttt agacgtacac atgaccctct tttatttcaa aggagctttt
     8761 tacagtattt ccaaaaggat ttttggtatg agatatgttt ttaaacatat attgtcaaaa
     8821 aatgaggcca attttagaga agagggctca caaaagtaca aagtattagg ttatatccta
     8881 ttagctcaaa atgttatgaa atggtaccct gtcctaacct ctacgctcgg gtcctggatt
     8941 tatgggagaa aaaggaccaa tgattcaatt actaggtcgt ctgttggtct acaggagcgt
     9001 tcagaacatg aatctataga aggcataccg aaggaatcgc agctgactca tataaatttg
     9061 tcagataaaa accaattgcc atttattcca gaagcctcga gaaaatgcat cctatgtctc
     9121 atgaacatga gtgatcctag ctgcgcgcca tgtggacatc tattttgttg gagttgtctt
     9181 atgagctggt gtaaagagag gccagaatgc cccttgtgca gacaacactg tcaaccacag
     9241 gaaattctgg tcctgcggca atagggatcc taactcgaga gtgcttttaa ctaagaatta
     9301 ttagtctttt ctgcttattt tttcatcata gtttagaaca ctttatatta acgaatagtt
     9361 tatgaatcta tttaggttta aaaattgata cagttttata agttactttt tcaaagactc
     9421 gtgctgtcta ttgcataatg cactggaagg ggaaaaaaaa ggtgcacacg cgtggctttt
     9481 tcttgaattt gcagtttgaa aaatgctgaa gtgaataaag ctccacacag tcgaacgaag
     9541 gcaagcccag aaaaatatcg caagcacctt tggtcttaca gtgccaactt ttggcctgcc
     9601 gacgttaaga gtacaaagct gatggcaatg tacgacaaga taacagagtc tcaaaagaag
     9661 tgaaacaatt tttcttcacc acattttcca ttgttccttc cccccataac tataaacgta
     9721 tttatgtata tatatttgcg tgtaagtgtg tgtactatag ggcaccgtaa agtaataatg
     9781 cttaattagt tactactatg accatataag aggtcatact gtatgaagcc acaaagcaga
     9841 tagatcaatc atgtttaacg aaaactgtta atcgaagatt atttcttttt ttttttctct
     9901 ttccttttta caaagaaaat tttttttgcg ctttttgcca tcaccatcgc aagttctggg
     9961 acaattgttc tctttcgctc cagttccaag gaaagaggtt tctgttttac ttaatagaaa
    10021 gtgtcatctt gtattttata tctcttcttt cttgtgtaaa attctttagt tttgattttg
    10081 tatttttagg acagtgagct acgaagtaac atttttactt aataaccgtt tgaagcatag
    10141 agcaggccct ggtatcacca cctaatatct ggctttttat tcaataaaaa ctcaaaaaaa
    10201 aaaatccaaa aaaaactaaa aaaccaataa aaataaaaga tctatgagct tttattcaaa
    10261 cctaccgtct gcaggacaat ctagtcgagg gtcgagtaca agcggtcgaa atggggtggg
    10321 attagaacca ttgtatccga ctatctttga gataatgtca tcacaagaaa tcgattcgtt
    10381 actgcccgca tccatccgtt atctactggc aaatcattta gtggcgaact tccctaacag
    10441 gtatactttg cggctgaaca agtacttttt tgagtggttt caagcgataa aagggtttgt
    10501 tgaatggtat catctaaaga catacaattc tacgtttatt gatcggtttt atggattgca
    10561 actatttagt tcgagggaca gaaatcttgc cttaacacaa tgtttaaatc caaagggcca
    10621 gagtgaatgg ccccaaggtt tgcagctgaa tcaacagcaa aagagtgtta tttttttgga
    10681 aaaaattatt ctcccctata tcacagcgaa gttggatgaa atattagaga aaatatcaat
    10741 gaataatata ttcagcagcg atgaaactga aaataaatgg cccaagagag cgtttctaag
    10801 aatttatcca tttattaaga aattattagc gttatccaat ttactcgtta aattattatt
    10861 tttaactaaa agaacagggt cggtttcctt gttgcaatac ttattcaaga tagagtacac
    10921 aacggtaaga ccactatcgt cagaactctc tggactcaaa gaaacgaaag ggatggataa
    10981 taggctaagg aaaacaaata tatcgtcaat tttcgcgcta atgcaaggac aattatcgat
    11041 tatacccaga tttttaacat ttatgggttc gcaattcttc cccactttta tatttgtcct
    11101 aagagtgtac caatggtgga ccacacaaga catgactact aaattgcaga aaagggtcaa
    11161 tgacttggat gaagatattc caaggccgcc tttctcctca catagtgaca aaacggaaga
    11221 taaagaggga gtttctgagg cctgtcccgt ttgcgaaaaa actgtacaaa acccctgtgt
    11281 attggaaaca ggatacgtgg catgctaccc gtgtgccata agctatttgg ttaataatga
    11341 aggccattgt cccgttacca ataaaaagct gctgggttgt acatacaaca aacacacgaa
    11401 taaatgggaa gttgtgacag gtattaggaa gctactaatc tgaggatcct aactcgagat
    11461 ttaactcctt aagttacttt aatgatttag tttttattat taataattca tgctcatgac
    11521 atctcatata cacgtttata aaacttaaat agattgaaaa tgtattaaag attcctcagg
    11581 gattcgattt ttttggaagt ttttgttttt ttttccttga gatgctgtag tatttgggaa
    11641 caattataca atcgaaagat atatgcttac attcgaccgt tttgctgagc agttacagag
    11701 atgttacgaa ccactagtgc actgcagtac atcgaggaga acttctagta tatctacata
    11761 cctaatatta ttgccttatt aaaaatggaa tcccaacaat tacatcaaaa tccacattct
    11821 cttcaaaatc aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg ttatatttat
    11881 aggataatta tactctattt ctcaacaagt aattggttgt ttggccgagc ggtctaaggc
    11941 gcctgattca agaaatatct tgaccgcagt taactgtggg aatactcagg tatcgtaaga
    12001 tgcaagagtt cgaatctctt agcaaccatt atttttttcc tcaacataac gagaacacac
    12061 aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta atttcagagg
    12121 tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa aggtgagagc
    12181 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
    12241 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
    12301 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatatt
    12361 tcaaggatat accattctaa tgtctgcccc taagaagatc gtcgttttgc caggtgacca
    12421 cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg
    12481 ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac
    12541 aggtgttcca cttccagatg aggcgctgga agcctccaag aaggctgatg ccgttttgtt
    12601 aggtgctgtg ggtggtccta aatggggaac cggtagtgtt agacctgaac aaggtttact
    12661 aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga
    12721 ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt
    12781 tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg
    12841 tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc
    12901 cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa
    12961 tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt
    13021 tcctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa
    13081 cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata tcatctccga
    13141 tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt
    13201 gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt
    13261 gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt
    13321 gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga
    13381 tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc
    13441 tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt
    13501 gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat
    13561 atgttcatag ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa
    13621 aaaggaggat gtaaaggaat acaggtaagc aaattgatac taatggctca acgtgataag
    13681 gaaaaagaat tgcactttaa cattaatatt gacaaggagg agggcaccac acaaaaagtt
    13741 aggtgtaaca gaaaatcatg aaactatgat tcctaattta tatattggag gattttctct
    13801 aaaaaaaaaa aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt
    13861 aagtcaatac cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact
    13921 ctgtcagaaa cggccttaac gacgtagtcg agagtactcg tatcgcatgt cggtgcgaca
    13981 cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata
    14041 catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact
    14101 aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga
    14161 gggcaccaca caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat
    14221 atattggagg attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc
    14281 tgaccatttg atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag
    14341 ttccctcaag aattttactc tgtcagaaac ggccttaacg acgtagtcga cctcctcttc
    14401 agtactaaat ctaccaatac caaatctgat ggaagaatgg gctaatgcat catccttacc
    14461 cagcgccgag cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
    14521 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
    14581 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
    14641 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
    14701 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
    14761 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
    14821 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgaggcga
    14881 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
    14941 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
    15001 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
    15061 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
    15121 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
    15181 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
    15241 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt
    15301 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
    15361 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
    15421 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc atgaccaaaa
    15481 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    15541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    15601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    15661 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    15721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    15781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    15841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    15901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    15961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    16021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    16081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    16141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    16201 ctgcgttatc ccctgattct gtggataacc gtgcggccgc caatacccag aagaacagta
    16261 aaataaagca aggtacgtga aattaatatt tttaaatggt tctaaccgat gccgaagaac
    16321 tgcgcagtcc ggttataacg tctgacatgt ccttttttga tttggaatcc aaccactcaa
    16381 gtgactctgt tcatttactt tgcgaaaaat atacccacaa attgcccatc gaaagtgaat
    16441 cgcaaaccac cttcagactg gcaccgacaa agcaaagatt atacagacag agtactttat
    16501 acgtaccgtt aagtctcaag caaagggttt tcttatttac tgaacgggta aagagtatct
    16561 gggccggctt gccaagatgc aaaccgaata agtatttcaa agttgcattt gccttagccg
    16621 tcctgacacc attggctatt tggatatttt atattgactt tcgtgtacat tgatcacatc
    16681 gactgttcta ttggcaaatg aaccacgggc attgactatt tttcaggtta ctactatata
    16741 ttatcatcac gggcaaggat tgtaccctga attcgcatct agactga
//
