LOCUS       sequence-431889-       15569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex22, Pex6, Pex15, and Pex8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15569)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 15569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1578
FEATURES             Location/Qualifiers
     source          1..15569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10754..11161
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11162..12256
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(13287..14102)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(13287..14102)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     14010..14029
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     14094..14113
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      14327..14915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14816..14835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgaaggc aagcccagaa aaatatcgca agcacctttg
       61 gtcttacagt gccaactttt ggcctgccga cgttaagagt acaaagctga tggcaatgta
      121 cgacaagata acagagtctc aaaagaagtg aaacaatttt tcttcaccac attttccatt
      181 gttccttccc cccataacta taaacgtatt tatgtatata tatttgcgtg taagtgtgtg
      241 tactataggg caccgtaaag taataatgct taattagtta ctactatgac catataagag
      301 gtcatactgt atgaagccac aaagcagata gatcaatcat gtttaacgaa aactgttaat
      361 cgaagattat ttcttttttt ttttctcttt cctttttaca aagaaaattt tttttgcgct
      421 ttttgccatc accatcgcaa gttctgggac aattgttctc tttcgctcca gttccaagga
      481 aagaggtttc tgttttactt aatagaaagt gtcatcttgt attttatatc tcttctttct
      541 tgtgtaaaat tctttagttt tgattttgta tttttaggac agtgagctac gaagtaacat
      601 ttttacttaa taaccgtttg aagcatagag caggccctgg tatcaccacc taatatctgg
      661 ctttttattc aataaaaact caaaaaaaaa aatccaaaaa aaactaaaaa accaataaaa
      721 ataaaagatc tatgccacca ccatcaagaa gtagaataaa caaaacaaga acattaggaa
      781 tagtgggtac agctatagca gtgttggtca cgtcctacta tatatatcaa aaggtgacaa
      841 gtgcaaagga agataatggg gcacgacctc cagagggtga ttcagtaaaa gagaacaaaa
      901 aggcaaggaa gagcaaatgt attataatga gcaagtcgat acaaggactg cccataaagt
      961 gggaggagta cgccgctgat gaagtggttt tgctggtacc tacgagccac actgatggat
     1021 caatgaaaca agccattggg gatgcctttc gcaagacgaa aaacgaacac aaaatcatat
     1081 attgcgatag catggatgga ttatggtcat gtgtaagacg gctaggtaaa tttcagtgca
     1141 tattgaactc cagggacttc acaagtagtg gtggtagcga tgcagcagtc gttcctgaag
     1201 atataggcag gtttgtcaaa tttgttgttg atagcgatgt agaggatgtg ctgattgaca
     1261 ctttatgcaa ttaaggatcc taactcgagg ccaattggtg cggcaattga taataacgaa
     1321 aatgtctttt aatgatctgg gtataatgag gaattttccg aacgttttta ctttatatat
     1381 atatatacat gtaacatata ttctatacgc tatagagaaa ggaaattttt caattaaaaa
     1441 aaaaatagag aaagagtttc acttcttgat tatcgctaac actaatggtt gaagtactgc
     1501 tactttaatt ttatgctgcc aaaccagatg tcaacacagc tacaacgttt gagaatgacc
     1561 ttccacgagc taaattgaaa gggaagaatt tattagttga actcaagaaa gaagaggatg
     1621 acgtgggaaa tggcatagaa tcccttacta aatcgaacac taaactgaac tccatgctgg
     1681 cgaacgaagg taagatacac aaagctagtt tccagaaaag tgtaaaattt aaactacctg
     1741 ataatatagt gactgaagaa accgtggaac ttaaagaaat aaaggacttg ctactacaaa
     1801 tgttgagatg acagcgagag attgaatcaa gattatccaa tatcgaactt caactcacgg
     1861 aaataccgaa acataagtaa tcatatccct tctcacattt tttacacagg aagtaagcaa
     1921 gttatgttat atttccgaca ctataattaa ttcttagcag ttaaaggtgc tttgtctata
     1981 ttacatttac atacagcttg agtgatcctg accggatata gggtcctatt ttcttacgtg
     2041 aacggctttt cttcttgttc ccgatggcct tcatgtgaaa aagcactcct cgggaggcgg
     2101 aaaaatatca aaagtacggg gcgaagttta taatgaagat ttatcgatat aaattttggt
     2161 tatttcagga gaacaagaaa gctctttaca ctaaaattat cagagaagaa gctgatatat
     2221 tagccctaag gagtacacca aaacacaaga tctatgaagg catcgcttac gtttagtctc
     2281 tccggaatat acgccccctg tagcatttct agggatatat atctcgaata cggcgataaa
     2341 aaagctgaat gtttatatgg gacaattaga ttgcctcagt acggtccagg ttgcacgcct
     2401 ggtaaaattg tacattgtgt cctggatgat tcactgccct tctgtagcat tgttgtacct
     2461 tccaaacttt ttgggtttat gccgacacag cccactatgg atttttgcta ttttgaaccc
     2521 atattagaca atgtagtacc tgtactggat agcgtaacgt ttctgattaa tgaacaattg
     2581 tactcaaaac taatggacct accgcaagag atgcaacaaa tacaattttt gcattacaaa
     2641 tataacatca actccatgga aaccgtggtt cattctcgag atattttaac atctggtctt
     2701 tgccaaattt taaactgctc acctttcccg cagggccttg ttgattttac agaaacccaa
     2761 ttgatattag taaatgatac tgagcagaaa ctaagtgctc taaaatatgc taacgaagat
     2821 gaagaatatg cattacctaa aatagggaca aattctgctt tatcaattga tcttgaatct
     2881 ctcccctgca caatttcaag agatcttttg cgacctgcac ctcacatcaa tgatgataat
     2941 tcaatatatg cctttacaga cgctgaaact ctactgcgcc tggatgtcac aagtggatcg
     3001 tttataacag tgtcaaatat gggttgcgtc aggctagtta agctgtttgt tttactactc
     3061 cccaacggct ttaaaaaaag aacaatatac gcacctccca aaataatagc aagctttcca
     3121 gactgcagcg tcgtgacaat atcaaagtcc aatataggcc atacagatat tcccattgct
     3181 aatcaagttt tcatctcaag agttggaggc tggcttcaat ctcagaaatg ctttcaaaat
     3241 attattctaa caaccttgaa aaagtttttt agcgagagca aaaggattct ttgtcaaaat
     3301 gatttaatcc caatcgcttt tgattcaagt atggctgatc tcaatatagc agaggaaaat
     3361 gatgagtctg atgacgaaga tgagctaggg cagtactaca agaacgattc ccttgtatgg
     3421 ttctttgtca caagcgcaga gctcgattgc ttctctaaag ataattctca ttttattatt
     3481 gatcccaatc ggacgaagct aataacaact aatatcacta acagaagacc attacccttg
     3541 agtagatcca atcttcagag atattacggt tttgctgaaa ctttttacta tgatttgcat
     3601 atcttcccct atgtaagaca attggttaac atattagaaa cttccttcaa ttgttctcaa
     3661 aggggaatca ccctaaatgc ctctgtgtta cttcattcca ctacaaacaa tgtgggcaag
     3721 gctacaatgg tgagatttgc ttcaaaatat cttggtatac atctactgga gatagattgc
     3781 ctttcgctca cttcaaattc aagacaactg gattcaacat ctaagattat tggttacatt
     3841 agggctaaat gtgaaaatgt cctaccgtac gcatcacctg ctgtgatttt tttggctcat
     3901 ttggattcaa ttttgcttga cgtaaacgct aatcaagatc cagaggcaat caaattacaa
     3961 aaatctataa acttcgaaat gtctaaactt ctggacgatt ttacgtttaa attcccagga
     4021 actacatttg ttggctccgt aaataacata gataacgtgc cttccagctt tagatcacat
     4081 atgagatttg agattctcgt acccgttccg tctgaagcac agagactgcg tatctttcag
     4141 tggtacttat cttcacatga actaaacaga gatgttcaac aaaaggttcc agtatcttac
     4201 atggataata tttcattttc atcactctct tcatattctg ctggtttaac tcctttagac
     4261 atcaaatcaa ttgtggaaac ggcacgaatg acggctactg cgcgttttta ccaagaatca
     4321 aagaagtgtg gatggcttcc gcaatcaatt ttgatcactc aggaggattt atcaaaagct
     4381 acttcgaaag ctaggaacga attctcggtt tcgattggtg ccccacaaat ccctaacgta
     4441 acttgggatg atataggtgg tattgatttt gttaaaggtg aaatattgga cacaatagac
     4501 atgccgctaa aacatcctga attatttacc tcaggtatga aaaagagaag cggtattctt
     4561 ttttatggtc caccgggtac aggtaaaact ctaatggcta aggccattgc aacaaatttt
     4621 tctttaaatt tttttagtgt taaaggccct gaactgttga atatgtacat tggtgagagt
     4681 gaagctaatg tgcgcagagt gtttcaaaag gcgagagagg ctaaaccttg tgtcatattt
     4741 ttcgatgaaa tcgattcagt agcacccaaa cgtggtaatc aaggtgattc gggtggtgtt
     4801 atggatcgta tcgtttcaca gttactagca gagttagatg gtatgagtac cgacgctgac
     4861 ggtgtatttg ttatcggagc aacaaacaga ccagacttat tggacgaagc actactaaga
     4921 ccaggacgat tcgataaatt gttatattta ggcattccgg atacggatac caaacaattg
     4981 aatattttag aggctctgac tcgcaaattc gtgctcgaca acgacgtaaa gcttattgag
     5041 ttggcaaagc tatgtccatt taattacacc ggggcagatt tttatgctct ctgctcagat
     5101 gcaatgctta acgccatgtc aagaattgca cgcatggtag aaaaaaaagt ttctcagcat
     5161 aacgaattga cgggagagaa tatttctaca cgtcgctggt ttgataagat tgcgacaaaa
     5221 gaggatacta aggttgtcgt aaaaatggaa gacttcttga aagcacaaga gcagctcacc
     5281 ccaagtgtgt cgcgggctga actgaatcat tatgaagcgg tgagagctaa ttttgaaggt
     5341 gcttaaggat cctaactcga gatttaactc cttaagttac tttaatgatt tagtttttat
     5401 tattaataat tcatgctcat gacatctcat atacacgttt ataaaactta aatagattga
     5461 aaatgtatta aagattcctc agggattcga tttttttgga agtttttgtt tttttttcct
     5521 tgagatgctg tagtatttgg gaacaattat acaatcgaaa gatatatgct tacattcgac
     5581 cgttttgctg gatgcacaca ctggcttaag atgacaacgt aagggcatga tagaattgga
     5641 ttatgtaaaa ggtgaagata ccattgtaga agcaaccagc acgtcgccgt ggctgatgaa
     5701 gtctcctctt gcccgggccg cagaaaagag gggcagtggc ctgtttttcg acataaatga
     5761 ggggcatggc cagcaccaag acgtcattgt tgcatatggc gtatccaagc cgaaacggcg
     5821 ctcgcctcat ccccacggga ataaggcagc cgacaaaaga aaaacgaccg aaaaggaacc
     5881 agaaagaaaa aagagggtgg gcgcgccgcg gacgtgtaaa aagatatgca tccagcttct
     5941 atatcgcttt aactttaccg ttttgggcat cgggaacgta tgtaacattg atctcctctt
     6001 gggaacggtg agtgcaacga atgcgatata gcaccgacca tgtgggcaaa ttcgtaataa
     6061 attcggggtg agggggattc aagacaagca accttgttag tcagctcaaa cagcgattta
     6121 acggttgagt aacacatcaa aacaccgttc gaggtcaagc ctggcgtgtt taacaagttc
     6181 ttgatatcat atataaatgt aataagaagt ttggtaatat tcaattcgaa gtgttcagtc
     6241 ttttacttct cttgttttat agaagaaaaa acatcaagaa acatctttaa catacacaaa
     6301 cacatactat cagaatacaa gatctatggc tgcaagtgag ataatgaaca atctgcctat
     6361 gcattccctc gattcttctc taagggattt acttaacgat gacttattca ttgaaagtga
     6421 tgaatcgacc aagtcggtaa atgatcagag gagtgaagta tttcaagaat gtgtgaacct
     6481 tttcataaaa agagatatca aggattgcct agaaaaaatg tctgaggttg gatttattga
     6541 tattaccgtt ttcaagtcca accctatgat attagattta tttgtcagcg cctgtgatat
     6601 tatgcccagc tttaccaaat taggtttaac actgcagagt gagattttga atattttcac
     6661 gttagatacc cctcaatgta ttgagacaag aaaaattatt ctcggtgacc tcagtaaact
     6721 tttggtcatc aataaattct ttcgatgctg tatcaaggtt attcagttta acttaactga
     6781 tcacaccgaa caagaagaaa aaactctgga gcttgaatcc ataatgagtg atttcatttt
     6841 cgtatacata acgaaaatga ggacaactat agatgtagtt ggcttacaag aattaatcga
     6901 gatattcatc tttcaggtga aggtaaaact gcatcataaa aaaccctcac cgaatatgta
     6961 ttgggcgctt tgcaaaacat tacctaagtt atctccaacc ctaaagggcc tttacttatc
     7021 taaagatgtt tccatagagg atgctattct gaactcaata gataataaaa tacaaaaaga
     7081 taaggcgaag tctaaaggta aacaaagagg agtgaaacag aaaatacacc attttcacga
     7141 gcctatgtta cacaatagta gtgaagaaca ggttaaggtt gaggatgcgt ttaatcaacg
     7201 cacctctact gatagtagac tccagagtac tggaacggcg ccccgtaaaa aaaacaatga
     7261 tattactgtt ctggctggtt cattttgggc tgtcctgaag catcatttca caagaagtgt
     7321 gctgaacaaa aacggacttc tgctcacagg tctgctgcta ctcttatgtt tgaaaaaata
     7381 taagtcattg atggcaattt tcaaacatgt tccagcggcc tttcacactg tataccccca
     7441 gattgtaggg ttgctaaaac ttctagcgag tatatgagga tcctaactcg agggagattg
     7501 ataagacttt tctagttgca tatcttttat atttaaatct tatctattag ttaatttttt
     7561 gtaatttatc cttatatata gtctggttat tctaaaatat catttcagta tctaaaaatt
     7621 cccctctttt ttcagttata tcttaacagg cgacagtcca aatgttgatt tatcccagtc
     7681 cgattcatca gggttgtgaa gcattttgtc aatggtcgct ggttctgtat gggcacagac
     7741 aacctaaacg tatatattaa atttgctctt gttctgtact ttcctaattc ttatgtaaaa
     7801 agacaagaat ttatgatact atttaataac aaaaaactac ctaagaaaag catcatgcag
     7861 tcgaaattga aatcgaaaag taaaacttta acggaacatg tttgaaattc taagaaagca
     7921 tacatcttca tcccttatat atagagttat gtttgatatt agtagtcatg ttgtaatctc
     7981 tggcctaagt atacgtaacg aaaatggtag cacgtcgcgt ttatggcccc caggttaatg
     8041 tgttctctga aattcgcatc actttgagaa ataatgggaa caccttacgc gtgagctgtg
     8101 cccaccgctt cgcctaataa agcggtgttc tcaaaatttc tccccgtttt caggatcacg
     8161 agcgccatct agttctggta aaatcgcgct tacaagaaca aagaaaagaa acatcgcgta
     8221 atgcaacagt gagacacttg ccgtcatata taaggttttg gatcagtaac cgttatttga
     8281 gcataacaca ggtttttaaa tatattatta tatatcatgg tatatgtgta aaattttttt
     8341 gctgactggt tttgtttatt tatttagctt tttaaaaatt ttactttctt cttgttaatt
     8401 ttttctgatt gctctatact caaaccaaca acaacttact ctacaactaa gatctatgtt
     8461 tgatcatgac gtcgaatatc tgatcactgc actctcttcc gaaacgagga tccagtatga
     8521 tcaacgcctg ctagacgaaa tcgcggccaa tgtggtatac tacgtcccaa gagtcaaatc
     8581 ccccgacact ttgtatcgct tggtcggagc acttttccga tcgcagttca ttgtccaatt
     8641 accaccacta cgccttttgc acatcgtcaa agatgttttc ctttggaaac tggaagtttc
     8701 tgagcccacg ctcccaattt cgaagtttta cttggtttgg aatgctgttt tcgaatctca
     8761 ccgagccacg tggaaccttt cacagttgat ggttttggat ggtgtactag tcacataccc
     8821 gagcttcaaa caattgaaca acgcctattt tattgatgaa tccagcaaca aaactgcgct
     8881 ttattacaga aactggaaat tacaactgtt ttcaccaata tgggcgcagc tatggaatac
     8941 tgccattgtc agggctaatt tatcgattca gcattgcctt ctgatagcat tagctttact
     9001 ttttaatcag tcaaaccgaa gcgccctttt gcatggagtt gacgtatcat ggaatttggt
     9061 cacagagaaa ctattggacc ttttggaaga gtatgtacac ggcattgtgc agccgatgga
     9121 aattttttcg actgattcag tcttatcaac caacttgaat catttggcca gttgcttgac
     9181 cagttcaatc acgaggtcaa atgaggcaac tttggtaaat tcagtacgca aactagaaag
     9241 gatatgtcga tacttgtccg atactgtggc ttcgttgaaa gaacaacaac tagatttcaa
     9301 gttccaaaat gtatttatcc tgataatcct cgcgttaaaa gaattatccg ccatgaatat
     9361 gacaatactg cctaaccata aagacacttt ttattctatg atctgtttaa gccttttcca
     9421 cgtccacgta ttgactcaaa aaatcgggac agtcggtttt cccagttacg attatgttta
     9481 cgataattta gtaacgtatt tcattgtcat ggatgacttg tctaaaatca ctacagtttt
     9541 ggaactaatg aaaagaaaca acaccaaaca agatccaaat aaattagtgt tttacatcaa
     9601 ttttctaaac aaaattacta attactatgg gtgtcgcatt cgtttgccgt ttattactga
     9661 gtttatcgaa cctttactgc atttcgatgt attttttagc ggaaagacgg gcaatacctt
     9721 ggatattgaa atcaaagaat ctatacacac attgacaatc actgtactgt ctatagactc
     9781 ctcttattcc tcacaggttg cacaatggca agtatcccgc atactcgtct atttaaagat
     9841 gtcgatggac caatttattg caggtaaatt gtccgcaaat caaattttac ttatattcgg
     9901 ccatctatcc acacaacttc cctcactaca taattataac aagcatttgc ttagggactc
     9961 tttgcacgaa acatatatca gaattgttaa tgtaaaaaac ccagaaaaga agaatgtatt
    10021 gattgaatgt ctgattgtac aaatagcctt tatcaacaat cctcatcatt tgatcggctg
    10081 gttgaacatc tgtctccagc taatcaatac acataacaaa aagctacttc aacaattgtg
    10141 ggaaatggtt tcgagtctag aatcctccct agccatcgac tggtggtaca caacggtctt
    10201 atcaagtcaa tcttctaaat tatagggatc ctaactcgag agtgctttta actaagaatt
    10261 attagtcttt tctgcttatt ttttcatcat agtttagaac actttatatt aacgaatagt
    10321 ttatgaatct atttaggttt aaaaattgat acagttttat aagttacttt ttcaaagact
    10381 cgtgctgtct attgcataat gcactggaag gggaaaaaaa aggtgcacac gcgtggcttt
    10441 ttcttgaatt tgcagtttga aaaatgctga gcagttacag agatgttacg aaccactagt
    10501 gcactgcagt acatcgagga gaacttctag tatatctaca tacctaatat tattgcctta
    10561 ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc
    10621 tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat
    10681 ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat
    10741 cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
    10801 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
    10861 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
    10921 tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca
    10981 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
    11041 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
    11101 ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat ataccattct
    11161 aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac
    11221 agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga
    11281 tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga
    11341 tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc
    11401 taaatgggga accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact
    11461 tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc
    11521 tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg
    11581 aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga
    11641 acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca
    11701 acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag
    11761 attatggaga aaaactgtgg aggaaaccat caagaacgaa tttcctacat tgaaggttca
    11821 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
    11881 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
    11941 aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
    12001 atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa
    12061 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
    12121 agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
    12181 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
    12241 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
    12301 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
    12361 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
    12421 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
    12481 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
    12541 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
    12601 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
    12661 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
    12721 acgacgtagt cgagagtact cgtatcgcat gtcggtgcga cacgaaatta caaaatggaa
    12781 tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga
    12841 aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa
    12901 ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt
    12961 taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc
    13021 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
    13081 taagtcaata ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac
    13141 tctgtcagaa acggccttaa cgacgtagtc gacctcctct tcagtactaa atctaccaat
    13201 accaaatctg atggaagaat gggctaatgc atcatcctta cccagcgccg agcggccgcg
    13261 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
    13321 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
    13381 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
    13441 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
    13501 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
    13561 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
    13621 accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta
    13681 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
    13741 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
    13801 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
    13861 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
    13921 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
    13981 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
    14041 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
    14101 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
    14161 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
    14221 aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt
    14281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    14341 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    14401 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    14461 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    14521 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    14581 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    14641 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    14701 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    14761 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    14821 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    14881 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    14941 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    15001 ctgtggataa ccgtgcggcc gccaataccc agaagaacag taaaataaag caaggtacgt
    15061 gaaattaata tttttaaatg gttctaaccg atgccgaaga actgcgcagt ccggttataa
    15121 cgtctgacat gtcctttttt gatttggaat ccaaccactc aagtgactct gttcatttac
    15181 tttgcgaaaa atatacccac aaattgccca tcgaaagtga atcgcaaacc accttcagac
    15241 tggcaccgac aaagcaaaga ttatacagac agagtacttt atacgtaccg ttaagtctca
    15301 agcaaagggt tttcttattt actgaacggg taaagagtat ctgggccggc ttgccaagat
    15361 gcaaaccgaa taagtatttc aaagttgcat ttgccttagc cgtcctgaca ccattggcta
    15421 tttggatatt ttatattgac tttcgtgtac attgatcaca tcgactgttc tattggcaaa
    15481 tgaaccacgg gcattgacta tttttcaggt tactactata tattatcatc acgggcaagg
    15541 attgtaccct gaattcgcat ctagactga
//
