LOCUS       sequence-431902-       17168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Tet-On inducible CRISPRai orthogonal system with 
            VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On 
            transactivator. PiggyBac vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17168)
  AUTHORS   Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, 
            Parker KR, Shy BR, Marson A, Chang HY
  TITLE     Bidirectional epigenetic editing reveals hierarchies in gene 
            regulation.
  JOURNAL   Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3.
  PUBMED    38760566
REFERENCE   2  (bases 1 to 17168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3."
COMMENT     Sequence Label: p101_CRISPRai_PiggyBac
FEATURES             Location/Qualifiers
     source          1..17168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..379
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             398..967
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             4148..4174
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4154..4180
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4193..4213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4220..4240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4289..4345
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4346..4372,4373..4375,4376..6862,6863..6865,
                     6866..8449)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4346..8449
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8453..8479
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8459..8485
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             8498..8518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8525..8545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8585..9280
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             9326..9511
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     polyA_signal    9537..9671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9574..9593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9628..9647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    9720..10309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9826..9837
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10236..10256
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10371..11117)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(11130..11183)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(11193..11564)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     intron          11601..12523
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(11624..11644)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     repeat_region   12878..12940
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12987..13007)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12987..13005)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13026..13042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13026..13042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13039..13061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13050..13066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13074..13080,13081..13098,13099..13104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13119..13140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13257..13274)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13428..14016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13508..13527)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14187..14978,14979..15047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14810..14829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15048..15152)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15178..15633)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15315..15336)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15527..15546
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15760..15782
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15774..15791
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15775..15791
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15801..15820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15801..15819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   15849..15883
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    16202..16220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16238..16256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16274..16292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16310..16328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16346..16364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16382..16400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    16418..16436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     16481..16505
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             16582..16731
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 tccgaagccg acgcaggccg gagagggaac actctccgaa gcacttcttc aactccagtt
       61 tgatgacgag gatcttggag cactccttgg aaactcgaca gaccctgcgg tgtttaccga
      121 cctcgcgtca gtagataact ccgaatttca gcagcttttg aaccagggta tcccggtcgc
      181 gccacataca acggagccca tgttgatgga ataccccgaa gcaatcacga gacttgtgac
      241 gggagcgcag cggcctcccg atcccgcacc cgcacctttg ggggcacctg gcctccctaa
      301 cggacttttg agcggcgacg aggatttctc ctccatcgcc gatatggatt tctcagcctt
      361 gctgtcacag atttccagcg gctctggcag cggcagccgg gattccaggg aagggatgtt
      421 tttgccgaag cctgaggccg gctccgctat tagtgacgtg tttgagggcc gcgaggtgtg
      481 ccagccaaaa cgaatccggc catttcatcc tccaggaagt ccatgggcca accgcccact
      541 ccccgccagc ctcgcaccaa caccaaccgg tccagtacat gagccagtcg ggtcactgac
      601 cccggcacca gtccctcagc cactggatcc agcgcccgca gtgactcccg aggccagtca
      661 cctgttggag gatcccgatg aagagacgag ccaggctgtc aaagcccttc gggagatggc
      721 cgatactgtg attccccaga aggaagaggc tgcaatctgt ggccaaatgg acctttccca
      781 tccgccccca aggggccatc tggatgagct gacaaccaca cttgagtcca tgaccgagga
      841 tctgaacctg gactcacccc tgaccccgga attgaacgag attctggata ccttcctgaa
      901 cgacgagtgc ctcttgcatg ccatgcatat cagcacagga ctgtccatct tcgacacatc
      961 tctgtttcat atgggtggag gctccgggaa gcggaactac atcctgggcc tggccatcgg
     1021 catcaccagc gtgggctacg gcatcatcga ctacgagaca cgggacgtga tcgatgccgg
     1081 cgtgcggctg ttcaaagagg ccaacgtgga aaacaacgag ggcaggcgga gcaagagagg
     1141 cgccagaagg ctgaagcggc ggaggcggca tagaatccag agagtgaaga agctgctgtt
     1201 cgactacaac ctgctgaccg accacagcga gctgagcggc atcaacccct acgaggccag
     1261 agtgaagggc ctgagccaga agctgagcga ggaagagttc tctgccgccc tgctgcacct
     1321 ggccaagaga agaggcgtgc acaacgtgaa cgaggtggaa gaggacaccg gcaacgagct
     1381 gtccaccaaa gagcagatca gccggaacag caaggccctg gaagagaaat acgtggccga
     1441 actgcagctg gaacggctga agaaagacgg cgaagtgcgg ggcagcatca acagattcaa
     1501 gaccagcgac tacgtgaaag aagccaaaca gctgctgaag gtgcagaagg cctaccacca
     1561 gctggaccag agcttcatcg acacctacat cgacctgctg gaaacccggc ggacctacta
     1621 tgagggacct ggcgagggca gccccttcgg ctggaaggac atcaaagaat ggtacgagat
     1681 gctgatgggc cactgcacct acttccccga ggaactgcgg agcgtgaagt acgcctacaa
     1741 cgccgacctg tacaacgccc tgaacgacct gaacaatctc gtgatcacca gggacgagaa
     1801 cgagaagctg gaatattacg agaagttcca gatcatcgag aacgtgttca agcagaagaa
     1861 gaagcccacc ctgaagcaga tcgccaaaga aatcctcgtg aacgaagagg atattaaggg
     1921 ctacagagtg accagcaccg gcaagcccga gttcaccaac ctgaaggtgt accacgacat
     1981 caaggacatt accgcccgga aagagattat tgagaacgcc gagctgctgg atcagattgc
     2041 caagatcctg accatctacc agagcagcga ggacatccag gaagaactga ccaatctgaa
     2101 ctccgagctg acccaggaag agatcgagca gatctctaat ctgaagggct ataccggcac
     2161 ccacaacctg agcctgaagg ccatcaacct gatcctggac gagctgtggc acaccaacga
     2221 caaccagatc gctatcttca accggctgaa gctggtgccc aagaaggtgg acctgtccca
     2281 gcagaaagag atccccacca ccctggtgga cgacttcatc ctgagccccg tcgtgaagag
     2341 aagcttcatc cagagcatca aagtgatcaa cgccatcatc aagaagtacg gcctgcccaa
     2401 cgacatcatt atcgagctgg cccgcgagaa gaactccaag gacgcccaga aaatgatcaa
     2461 cgagatgcag aagcggaacc ggcagaccaa cgagcggatc gaggaaatca tccggaccac
     2521 cggcaaagag aacgccaagt acctgatcga gaagatcaag ctgcacgaca tgcaggaagg
     2581 caagtgcctg tacagcctgg aagccatccc tctggaagat ctgctgaaca accccttcaa
     2641 ctatgaggtg gaccacatca tccccagaag cgtgtccttc gacaacagct tcaacaacaa
     2701 ggtgctcgtg aagcaggaag aagccagcaa gaagggcaac cggaccccat tccagtacct
     2761 gagcagcagc gacagcaaga tcagctacga aaccttcaag aagcacatcc tgaatctggc
     2821 caagggcaag ggcagaatca gcaagaccaa gaaagagtat ctgctggaag aacgggacat
     2881 caacaggttc tccgtgcaga aagacttcat caaccggaac ctggtggata ccagatacgc
     2941 caccagaggc ctgatgaacc tgctgcggag ctacttcaga gtgaacaacc tggacgtgaa
     3001 agtgaagtcc atcaatggcg gcttcaccag ctttctgcgg cggaagtgga agtttaagaa
     3061 agagcggaac aaggggtaca agcaccacgc cgaggacgcc ctgatcattg ccaacgccga
     3121 tttcatcttc aaagagtgga agaaactgga caaggccaaa aaagtgatgg aaaaccagat
     3181 gttcgaggaa aagcaggccg agagcatgcc cgagatcgaa accgagcagg agtacaaaga
     3241 gatcttcatc accccccacc agatcaagca cattaaggac ttcaaggact acaagtacag
     3301 ccaccgggtg gacaagaagc ctaatagaga gctgattaac gacaccctgt actccacccg
     3361 gaaggacgac aagggcaaca ccctgatcgt gaacaatctg aacggcctgt acgacaagga
     3421 caatgacaag ctgaaaaagc tgatcaacaa gagccccgaa aagctgctga tgtaccacca
     3481 cgacccccag acctaccaga aactgaagct gattatggaa cagtacggcg acgagaagaa
     3541 tcccctgtac aagtactacg aggaaaccgg gaactacctg accaagtact ccaaaaagga
     3601 caacggcccc gtgatcaaga agattaagta ttacggcaac aaactgaacg cccatctgga
     3661 catcaccgac gactacccca acagcagaaa caaggtcgtg aagctgtccc tgaagcccta
     3721 cagattcgac gtgtacctgg acaatggcgt gtacaagttc gtgaccgtga agaatctgga
     3781 tgtgatcaaa aaagaaaact actacgaagt gaatagcaag tgctatgagg aagctaagaa
     3841 gctgaagaag atcagcaacc aggccgagtt tatcgcctcc ttctacaaca acgatctgat
     3901 caagatcaac ggcgagctgt atagagtgat cggcgtgaac aacgacctgc tgaaccggat
     3961 cgaagtgaac atgatcgaca tcacctaccg cgagtacctg gaaaacatga acgacaagag
     4021 gccccccagg atcattaaga caatcgcctc caagacccag agcattaaga agtacagcac
     4081 agacattctg ggcaacctgt atgaagtgaa atctaagaag caccctcaga tcatcaaaaa
     4141 gggcgcctat ccctatgacg tgcccgatta tgccagcctg ggcagcggct cccccaagaa
     4201 aaaacgcaag gtggaagatc ctaagaaaaa gcggaaagtg gacggcattg gtagtgggag
     4261 caacggcagc agcggatccg gaagcggagc tactaacttc agcctgctga agcaggctgg
     4321 agacgtggag gagaaccctg gacctatgga caagaagtac agcatcggcc tggccatcgg
     4381 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
     4441 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gcgccctgct
     4501 gttcgacagc ggagaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
     4561 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
     4621 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
     4681 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     4741 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
     4801 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
     4861 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     4921 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct
     4981 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga
     5041 gaagaagaat ggcctgttcg gcaacctgat tgccctgagc ctgggcctga cccccaactt
     5101 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga
     5161 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc
     5221 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat
     5281 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct
     5341 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt
     5401 cgaccagagc aagaacggct acgccggcta catcgatggc ggagccagcc aggaagagtt
     5461 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa
     5521 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca
     5581 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt
     5641 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     5701 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac
     5761 catcaccccc tggaacttcg aggaagtggt ggacaagggc gccagcgccc agagcttcat
     5821 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag
     5881 cctgctgtac gagtacttca ccgtgtacaa cgagctgacc aaagtgaaat acgtgaccga
     5941 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaagccatcg tggacctgct
     6001 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     6061 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     6121 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     6181 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     6241 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     6301 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     6361 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     6421 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     6481 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     6541 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     6601 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     6661 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     6721 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     6781 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     6841 gctgtccgac tacgatgtgg acgctatcgt gcctcagagc tttctgaagg acgactccat
     6901 cgataacaaa gtgctgactc ggagcgacaa gaaccggggc aagagcgaca acgtgccctc
     6961 cgaagaggtc gtgaagaaga tgaagaacta ctggcgccag ctgctgaatg ccaagctgat
     7021 tacccagagg aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     7081 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     7141 acagatcctg gactcccgga tgaacactaa gtacgacgag aacgacaaac tgatccggga
     7201 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     7261 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     7321 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     7381 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     7441 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     7501 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa caggcgagat
     7561 cgtgtgggat aagggccggg actttgccac cgtgcggaaa gtgctgtcta tgccccaagt
     7621 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     7681 caagaggaac agcgacaagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     7741 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     7801 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     7861 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     7921 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     7981 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     8041 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     8101 tgagcagaaa cagctgtttg tggaacagca caaacactac ctggacgaga tcatcgagca
     8161 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aggtgctgag
     8221 cgcctacaac aagcacagag acaagcctat cagagagcag gccgagaata tcatccacct
     8281 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     8341 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     8401 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgacg cctatcccta
     8461 tgacgtgccc gattatgcca gcctgggcag cggctccccc aagaaaaaac gcaaggtgga
     8521 agatcctaag aaaaagcgga aagtggacgg cattggtagt gggagcaacg gcagcagcgg
     8581 atccagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
     8641 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     8701 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     8761 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
     8821 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     8881 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     8941 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     9001 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
     9061 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
     9121 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
     9181 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
     9241 atactgcgac ctccctagca aactggggca caagcttaat ggcggtggcg gagggatgga
     9301 tgctaagtca ctaactgcct ggtcccggac actggtgacc ttcaaggatg tatttgtgga
     9361 cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcgtgt acagaaatgt
     9421 gatgctggag aactataaga acctggtttc cttgggttat cagcttacta agccagatgt
     9481 gatcctccgg ttggagaagg gagaagagcc ctaagacgaa tgcaattgtt gttgttaact
     9541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9661 atgtctggat caactggata actcaagcta accgtcatac atatgtcgac ggtatcgatg
     9721 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     9781 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     9841 gacgtagcag aaggacgtcc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
     9901 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     9961 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
    10021 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
    10081 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
    10141 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
    10201 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
    10261 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct
    10321 tgatatcaag cttgcatgcc tgcaggtcga ctctagagtc ttatgcggcc ttagcctggc
    10381 agcatatcga gatcgaagtc atcgagagcg tcagcaggga gcatatccag atcaaaatca
    10441 tccagggcat cggcgggcag catgtccagg tcgaagtcgt ccagggcgtc ggtgggtccg
    10501 ccgctctcgc acttcagctg cttctcgagg ccgcagatga tcagttccag gccgaacagg
    10561 aaggcgggct cggcgccctg tctgtcgaac agctcgatgg cctgcttcag cagggggggc
    10621 atgctgtcgg tggtgggtgt ctctctttcc tctttggcga cctggtgttc ctgttcttcc
    10681 agcacgcagc ccagggtgaa gtggcccacg gcgctcaggg cgtacagggc gttttccagg
    10741 ctgaagccct gctggcacag gaaggccagc tggttttcca gtgtctcgta ctgcttctcg
    10801 gtgggtctgg tgcccaggtg cactttggcg ccgtcccggt ggctcagcag ggcgcatctg
    10861 tagctcttgg cgttgttccg caggaagtcc tgccagctct cgccttccag agggcagctg
    10921 tgggtgtggt gccggtccag catctcgatg ggcagggcgt ccagcagggc ccgcttgttc
    10981 ttcacgtgcc agtacagggt gggctgttcc acgcccagtt tctgggccag cttccgggtg
    11041 gtcaggccct cgatgcccac gccgttcagc agttccaggg cgctgttgat cactttgctc
    11101 ttgtccagcc ggctcatcga ccctccgcct gggccaggat tctcctcgac gtcaccgcat
    11161 gttagcagac ttcctctgcc ctcggaaccg ccgtcctgct cctcggccac gaagtgcacg
    11221 cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg
    11281 gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg
    11341 tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg
    11401 tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc
    11461 tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg
    11521 tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccataattct cgagagctcc
    11581 agatcccctg ttcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc
    11641 ttgagaatga accaagatcc aaactcaaaa aggccaaatt ccaaggagaa ttacatcaag
    11701 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa
    11761 aacccctccc cccaacctaa agacgacgta ctccaaaagc tccagaacta atcgaggtgc
    11821 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc
    11881 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt
    11941 gagccccctg gagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac
    12001 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga
    12061 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc
    12121 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccggccgc gggcccaaaa
    12181 accgaaatac cagtgtgcag atcctggccc gcatttacaa gactatcttg ccagaaaaaa
    12241 agcgtcgcag cacgtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga
    12301 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca
    12361 actcaagcac gaggcgaagg ggctcctaaa gcgcaaggcc cgcccctggc tccagctcgg
    12421 gatcaagaat cacgtactgg agccaggtgg aagtaattca aggcacgcaa gggccataac
    12481 ccgtaaagag gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa
    12541 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
    12601 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
    12661 ttctctaggc accggttcaa ttggctgcag gactagtcta gttaaaagtt ttgttacttt
    12721 atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt
    12781 ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa
    12841 ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat
    12901 tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagt gtttaaacgg
    12961 ccgcttaatt aatccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    13021 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    13081 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    13141 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    13201 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    13261 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    13321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    13381 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    13441 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    13501 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    13561 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    13621 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    13681 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13741 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13801 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    13861 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    13921 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    13981 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    14041 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    14101 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    14161 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    14221 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    14281 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    14341 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    14401 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    14461 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    14521 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    14581 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    14641 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    14701 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    14761 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    14821 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    14881 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    14941 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    15001 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    15061 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    15121 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
    15181 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    15241 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    15301 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    15361 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    15421 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    15481 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    15541 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    15601 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
    15661 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    15721 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    15781 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggg cgcgccattc
    15841 tagattaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
    15901 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
    15961 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
    16021 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
    16081 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
    16141 ttcttgttat agatatcaac tagaatgcta gcgctgatat ctccacccca cagtggttta
    16201 ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt
    16261 atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt
    16321 gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta
    16381 ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt
    16441 ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc
    16501 agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg taccacttcc
    16561 taccctcgta aagaattcat ggatgcttta gacgattttg acttagatat gcttggttca
    16621 gacgcgttag acgacttcga cctagacatg ttaggctcag atgcattgga cgacttcgat
    16681 ttagatatgt tgggctccga tgccctagat gactttgatc tagatatgct aggtagtggc
    16741 ggcagcggat cccagtatct gcccgacaca gatgatagac accgaatcga agagaaacgc
    16801 aagcgaacgt atgaaacctt caaatcgatc atgaagaaat cgcccttctc gggtccgacc
    16861 gatcccaggc ccccaccgag aaggattgcg gtcccgtccc gctcgtcggc cagcgtgccg
    16921 aagcctgcgc cgcagcccta ccccttcacg tcgagcctga gcacaatcaa ttatgacgag
    16981 ttcccgacga tggtgttccc ctcgggacaa atctcacaag cctcggcgct cgcaccagcg
    17041 cctccccaag tccttccgca agcgcctgcc ccagcgcctg caccggcaat ggtgtccgcc
    17101 ctcgcacagg cccctgcgcc cgtccccgtg ctcgcgcctg gaccgcccca ggcggtcgct
    17161 ccaccggc
//
