LOCUS       sequence-431905-        5652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses TdTomato in Capsaspora owczarzaki.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5652)
  TITLE     Plasmids for generating fluorescent Capsaspora owczarzaki
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJG01
FEATURES             Location/Qualifiers
     source          1..5652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             304..1734
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     rep_origin      complement(2141..2729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2221..2240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2900..3691,3692..3760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3523..3542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3761..3865)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttcctccaac tgtcgttctg cgtttgttct tcgctctcgt cgtgcacact cggattagga
       61 tccattcatc cccatcccct tgcgcattgc caaaaacgag cgtcgaaacg cgaacgtcag
      121 cgtggggtgt tgacgccgaa ccccacgagt gttttttttt aaaatgctgt ttcatcgttt
      181 tcttccttct ccccacgtgc aatcctgatt taaataattg tcgctttttg atttggaaat
      241 gctcactctt ccattttttt attatttaac acacacagaa caaccttcac aactagtggt
      301 accatggtct cgaagggcga ggaggtcatc aaggagttca tgcgcttcaa ggtccgcatg
      361 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      421 gagggcaccc agaccgccaa gctgaaggtc accaagggcg gccccctgcc cttcgcctgg
      481 gacatcctgt ccccccagtt catgtacggc tccaaggcct acgtcaagca ccccgccgac
      541 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtcatgaac
      601 ttcgaggacg gcggtctggt caccgtcacc caggactcct ccctgcagga cggcaccctg
      661 atctacaagg tcaagatgcg cggcaccaac ttcccccccg acggccccgt catgcagaag
      721 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtcctgaag
      781 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt cgagttcaag
      841 accatctaca tggccaagaa gcccgtccag ctgcccggct actactacgt cgacaccaag
      901 ctggacatca cctcccacaa cgaggactac accatcgtcg aacagtacga gcgctccgag
      961 ggccgccacc acctgttcct gggccacggc accggctcga ccggctcggg ctcgtccggc
     1021 accgcctcct ccgaggacaa caacatggcc gtcatcaagg agttcatgcg cttcaaggtc
     1081 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1141 ccctacgagg gcacccagac cgccaagctg aaggtcacca agggcggccc cctgcccttc
     1201 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcctacgt caagcacccc
     1261 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtc
     1321 atgaacttcg aggacggcgg tctggtcacc gtcacccagg actcctccct gcaggacggc
     1381 accctgatct acaaggtcaa gatgcgcggc accaacttcc cccccgacgg ccccgtcatg
     1441 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtc
     1501 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccgcta cctggtcgag
     1561 ttcaagacca tctacatggc caagaagccc gtccagctgc ccggctacta ctacgtcgac
     1621 accaagctgg acatcacctc ccacaacgag gactacacca tcgtcgaaca gtacgagcgc
     1681 tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa gtaaggcggc
     1741 cgcattttgt gtttgccaag acacatttgt ttatttcaat catttctaag accaaaaagg
     1801 gatttcatga tttttgttgc ttgcattgaa gttttttttg gttgtcattt tttttgcgtt
     1861 ggttatgagc gcaatatatt ccattatggc aagaaacacg acgagttgtt tttacagtgg
     1921 agggatgagc aaggaatggg gaaatggaga acaagtggaa tgaaatggtt tgactttgtg
     1981 cggcgaaagt gttgccattt tcaatcatgc tgcgcctggc acctagatgc gtgtgattgc
     2041 tcattgctag tgctgttctc accgccggca tcggaaagaa catgtgagca aaaggccagc
     2101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcccaa
     3901 tgatcccaca aaaatgctga ttgtttgttt ttttccactg cggcttgcca tctttggaaa
     3961 ccgcagtcaa caaacaggtt tttcaggtca aactgccatg gcagactgac ctgaaatcgt
     4021 gctgcgaccc gctgggggta tttaaggcgg cccgttttgg acgagtttca gatcagtctt
     4081 ttaacctagt taaatcccac caacccacca aaatggatgg tgaagatgtt caagctggct
     4141 cgtttcgcat gattgaacaa gatggcctcc acgccggttc gcccgccgct tgggtcgagc
     4201 gcctcttcgg ctacgactgg gcccaacaga ccatcggctg ctcggatgcc gccgtcttcc
     4261 gcctgtcggc ccagggccgc cccgttctct ttgtcaagac cgacctgtcc ggtgccctga
     4321 acgaactgca ggacgaggcc gcccgcctct cgtggctggc caccaccggc gttccctgcg
     4381 ccgctgtcct cgacgttgtc actgaagccg gccgcgactg gctgctcctc ggcgaagtcc
     4441 ccggccagga tctcctgtcg tcgcacctcg ctcccgccga gaaggtctcc atcatggctg
     4501 atgccatgcg ccgcctgcac accctcgatc ccgctacctg ccccttcgac caccaagcca
     4561 agcaccgcat cgagcgcgcc cgtactcgca tggaagccgg tctcgtcgat caggatgatc
     4621 tggacgaaga gcaccagggc ctcgcccccg ccgaactgtt cgcccgcctc aaggcccgca
     4681 tgcccgacgg cgaggatctc gtcgtcaccc acggcgatgc ctgcctcccc aacatcatgg
     4741 tcgaaaacgg ccgcttttcg ggcttcatcg actgcggccg tctgggtgtc gccgaccgct
     4801 accaggacat cgccctcgct acccgtgata ttgctgaaga gctcggcggc gaatgggctg
     4861 accgcttcct cgtcctctac ggtatcgccg ctcccgattc gcagcgcatc gccttctacc
     4921 gcctcctcga cgagttcttc taagcgcgtt ctctccgagc gccctagtcg ccaacgctgc
     4981 ctgatgcgga ttgtgtctgt tgctgcgtga tcttgtacaa agaaaaaagg ggccggcaaa
     5041 tgcacagtta gcaacgaccg acaatcagca acctccaaac tctcagtttt tttcccccaa
     5101 tcgttgtcga tttagctttt gatgctgcat tttgaaatag aacgacaacc gaatttggga
     5161 gagagcactc ttttacaggt ttgtgaaaag aaaaaaaatc gaacgtgact tgcttccatc
     5221 ttccttctcg aagccgatct cggcccatcc ccgcttgtcc cctcctcccc tcctcgcaat
     5281 ccgctagaca gtgtctctgc acactggatt gcggtgcgcg ggccatccga tgcccctttc
     5341 ctcggcctgg agatggacgt gaaagtggaa aacgaaaaac gcgaaacaga aacatgtggt
     5401 tctttttttt ttctttttct ttttcgcctt cgggcttttc tctctcgctt cctcttctcc
     5461 gctctcttct tcgcgctctt ttgcctcttg cgtgagatgc tcgtcttgca atcgtctgcc
     5521 actgctgcgc tgtcacgatc gatcgcatcg gacaagccgt ttctgttttg aaatttagaa
     5581 cgttctggaa aacaacaaaa aattgccttt ttttcacttt tttctttttt cgtttttttc
     5641 cctcttcaac ac
//
