LOCUS       sequence-431906-        8193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1332
FEATURES             Location/Qualifiers
     source          1..8193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2561..2748
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2616..2640)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        3224..3631
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3632..4726
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5757..6572)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5757..6572)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6480..6499
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6564..6583
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6797..7385
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7286..7305
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgg acgtaggaag
      721 ttgctcagtg ggaaataatc cgcttgcgca gttgcacaaa catactcagc agaacaaatc
      781 gcttcagttt aatcagaaga ataatgggcg tcttaatgag tcacctctac agggtaccaa
      841 caagccaggt attagtgagg cttttatatc caatgttaat gctatttcac aagaaaacat
      901 ggcgaatatg caaaggttca taaacggaga accactgatc gatgataaaa gaagaatgga
      961 aatagggcca tcctcaggca ggcttccacc tttttcaaac gtacattctc tacagacttc
     1021 agcaaaccca acccaaatta agggagtgaa cgatatatct cattggtcac aggaatttca
     1081 aggtagtaat agtattcaaa atagaaacgc ggatacagga aattcagaaa aggcatggca
     1141 gcgtggctca acaaccgcat caagccggtt tcagtaccct aataccatga tgaataacta
     1201 tgcttatgct tctatgaaca gtcttagtgg atcaaggctc caatcgcctg ctttcatgaa
     1261 tcaacaacag tctggtcgtt ctaaagaagg agtcaatgag caagaacaac aaccctggac
     1321 agatcagttt gaaaagctgg aaaaagaagt ctcagaaaac ttggacataa atgatgaaat
     1381 agagaaggag gaaaatgtga gtgaagtaga acaaaacaaa ccagaaactg ttgagaagga
     1441 agaaggagta tatggagatc agtatcaatc tgatttccaa gaagtgtggg atagcataca
     1501 caaggacgct gaagaagtct tgccatccga attagttaat gatgacctca atctaggaga
     1561 agactacttg aaatatctcg gcggtagagt aaatgggaac atcgagtatg cttttcaatc
     1621 taacaacgaa tattttaata atcctaatgc ttataaaatt ggctgcctac tgatggaaaa
     1681 cggagccaaa ttgagcgagg cagcgctagc atttgaagct gctgttaaag aaaaaccgga
     1741 ccatgtggat gcatggctaa gattgggtct agtacaaacc cagaatgaaa aagagttgaa
     1801 cggcataagc gccctcgaag aatgtttaaa gttagaccca aagaatctgg aggcaatgaa
     1861 aactttagcg ataagttata taaacgaagg ttatgatatg agcgccttca caatgctgga
     1921 taaatgggca gaaactaagt acccggaaat ttggtcaagg atcaagcaac aagatgacaa
     1981 atttcaaaaa gagaaagggt ttacccatat tgatatgaac gctcatatca caaagcaatt
     2041 tttgcaacta gcaaacaatt taagcacaat agatcctgaa atacaactat gcttgggact
     2101 cttattttac acgaaagatg attttgacaa aaccatagat tgctttgaaa gtgcgttgag
     2161 ggtgaatcct aatgacgaac tcatgtggaa tagattaggg gcttcattgg ccaattccaa
     2221 tagatcagag gaagcaatcc aagcctatca tagggcacta caactaaaac cttcttttgt
     2281 tagagctcgc tataatctgg cggtatcatc catgaatata ggctgtttca aagaagcagc
     2341 aggctactta ttaagtgttc taagtatgca tgaagtgaac actaataata aaaaaggaga
     2401 tgttggatct ctcttgaata cgtacaatga tactgttata gagactttga agagagtttt
     2461 tatagcgatg aatagagatg atttacttca agaagtgaag ccaggcatgg acctgaaaag
     2521 atttaaagga gaattttcgt tttgaggatc ctaactcgag gcgaatttct tatgatttat
     2581 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     2641 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     2701 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa
     2761 atgcctgcaa atcgctcccc atttcgctgg aaatctgctc gtcagtggtg ctcacactga
     2821 cgaatcatgt acagatcata ccgatgactg cctggcgact cacaactaag caagacagcc
     2881 ggaaccagcg ccggcgaaca ccactgcata tatggcatat cacaacagtc cacgtctcaa
     2941 gcagttacag agatgttacg aaccactagt gcactgcagt acatcgagga gaacttctag
     3001 tatatctaca tacctaatat tattgcctta ttaaaaatgg aatcccaaca attacatcaa
     3061 aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg tgttcaaaaa
     3121 cgttatattt ataggataat tatactctat ttctcaacaa gtaattggtt gtttggccga
     3181 gcggtctaag gcgcctgatt caagaaatat cttgaccgca gttaactgtg ggaatactca
     3241 ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt cctcaacata
     3301 acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt
     3361 taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg
     3421 aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat
     3481 gcttgcatca caatacttga agttgacaat attatttaag gacctattgt tttttccaat
     3541 aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata
     3601 tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga tcgtcgtttt
     3661 gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat
     3721 ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc
     3781 tatcgatgct acaggtgttc cacttccaga tgaggcgctg gaagcctcca agaaggctga
     3841 tgccgttttg ttaggtgctg tgggtggtcc taaatgggga accggtagtg ttagacctga
     3901 acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa
     3961 ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac
     4021 tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga
     4081 cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat
     4141 cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt
     4201 ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat
     4261 caagaacgaa tttcctacat tgaaggttca acatcaattg attgattctg ccgccatgat
     4321 cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga
     4381 tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc
     4441 cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc
     4501 tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat
     4561 gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa
     4621 aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga
     4681 agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt
     4741 tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt
     4801 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
     4861 caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct
     4921 caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcacc
     4981 acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt tatatattgg
     5041 aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat
     5101 ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc
     5161 aagaatttta ctctgtcaga aacggcctta acgacgtagt cgagagtact cgtatcgcat
     5221 gtcggtgcga cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac
     5281 gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag
     5341 caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta acattaatat
     5401 tgacaaggag gagggcacca cacaaaaagt taggtgtaac agaaaatcat gaaactatga
     5461 ttcctaattt atatattgga ggattttctc taaaaaaaaa aaaatacaac aaataaaaaa
     5521 cactcaatga cctgaccatt tgatggagtt taagtcaata ccttcttgaa ccatttccca
     5581 taatggtgaa agttccctca agaattttac tctgtcagaa acggccttaa cgacgtagtc
     5641 gacctcctct tcagtactaa atctaccaat accaaatctg atggaagaat gggctaatgc
     5701 atcatcctta cccagcgccg agcggccgcg tgttacaacc aattaaccaa ttctgattag
     5761 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     5821 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     5881 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     5941 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     6001 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     6061 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     6121 tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     6181 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     6241 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     6301 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     6361 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     6421 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     6481 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg
     6541 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     6601 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     6661 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     6721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gccaataccc
     7501 agaagaacag taaaataaag caaggtacgt gaaattaata tttttaaatg gttctaaccg
     7561 atgccgaaga actgcgcagt ccggttataa cgtctgacat gtcctttttt gatttggaat
     7621 ccaaccactc aagtgactct gttcatttac tttgcgaaaa atatacccac aaattgccca
     7681 tcgaaagtga atcgcaaacc accttcagac tggcaccgac aaagcaaaga ttatacagac
     7741 agagtacttt atacgtaccg ttaagtctca agcaaagggt tttcttattt actgaacggg
     7801 taaagagtat ctgggccggc ttgccaagat gcaaaccgaa taagtatttc aaagttgcat
     7861 ttgccttagc cgtcctgaca ccattggcta tttggatatt ttatattgac tttcgtgtac
     7921 attgatcaca tcgactgttc tattggcaaa tgaaccacgg gcattgacta tttttcaggt
     7981 tactactata tattatcatc acgggcaagg attgtaccct gaattcgcat ctagactgat
     8041 gagacgtggt agagccacaa acagccggta caagcaacga tctccaggac catctgaatc
     8101 atgcgcggat gacacgaact cacgacggcg atcacagaca ttaacccaca gtacagacac
     8161 tgcgacaacg tggcaattcg tcgcaataca acg
//
