LOCUS       sequence-431938-        7689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB718
FEATURES             Location/Qualifiers
     source          1..7689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2057..2244
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2112..2136)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        2720..3127
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3128..4222
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(5253..6068)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5253..6068)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5976..5995
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6060..6079
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6293..6881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6782..6801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagaggatgt ccaatatttt ttttaaggaa taaggatact tcaagactag attcccccct
       61 gcattcccat cagaaccgta aaccttggcg ctttccttgg gaagtattca agaagtgcct
      121 tgtccggttt ctgtggctca caaaccagcg cgcccgatat ggctttcttt tcacttatga
      181 atgtaccagt acgggacaat tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt
      241 acattctaac catgtcacac tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc
      301 ttgagaacga tagattttct ttattttaca ttgaacagtc gttgtctcag cgcgctttat
      361 gttttcattc atacttcata ttataaaata acaaaagaag aatttcatat tcacgcccaa
      421 gaaatcaggc tgctttccaa atgcaattga cacttcatta gccatcacac aaaactcttt
      481 cttgctggag cttcttttaa aaaagacctc agtacaccaa acacgttacc cgacctcgtt
      541 attttacgac aactatgata aaattctgaa gaaaaaataa aaaaattttc atacttcttg
      601 cttttattta aaccattgaa tgatttcttt tgaacaaaac tacctgtttc accaaaggaa
      661 atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa agatctatgg ccccaaatca
      721 aagatcacgt tcgcttctgc agagacatcg aggaaaggta ctcatttcat tgactggaat
      781 agctgcttta tttaccacag ggtcggtggt agtgtttttc gtgaagagat ggttgtataa
      841 acagcagtta cggattactg agcaacactt catcaaagaa cagatcaaaa gaaggttcga
      901 gcagacacag gaagactcgt tgtacacaat atacgagcta cttcctgtat ggagaatggt
      961 tttgaatgaa aacgatttga atttggacag catcgttacc caattaaagg accaaaagaa
     1021 ccagttgact agagcaaagt ctagtgaaag cagagaaagt tcgccgttaa aaagtaaagc
     1081 tgagttgtgg aacgagttag aactaaagag tttgatcaag ctggtgacag tgacgtacac
     1141 agtatcgtcg ctgattcttt taacaagact gcaactaaat atcctgacaa gaaacgagta
     1201 cctggactcc gcaataaaat taaccatgca gcaggaaaac tgcaacaaac tacagaatag
     1261 gttctataac tgggttacat cgtggtggag cgatccggag gacaaagcgg atgatgcaat
     1321 ggtaatggca gcaaaaaagt caaagaaaga aggccaggaa gtgtatatta atgagcaagc
     1381 tttcctatca ctatcatggt ggattcttaa caaggggtgg ttgagttaca atgaaataat
     1441 tacaaatcaa atcgaaattg aatttgacgg catacaccca agagatactc taacgttaga
     1501 agaatttagt tctcgtctaa cgaacatatt ccggaacacg aattcccaaa tattccagca
     1561 aaataataat aacctcacgt ctattttgct tcctaaagat tccagtggac aggagtttct
     1621 tctgtcacag acgcttgacg cggatgcttt aacaagtttt cactctaaca cactagtttt
     1681 taatcaattg gtgaatgaac tgacccaatg tatcgaaagc acagcaacat ccatagtgtt
     1741 agagtctcta ataaatgaat cattccattt cataatgaac aaggtcggca taaagacaat
     1801 agccaagaaa aaacctggcc aagaagatca gcaacagtat caaatggcag ttttcgctat
     1861 gtcgatgaaa gactgttgcc aagaaatgct tcaaacaact gccgggtctt cccatagcgg
     1921 cagcgtgaac gaatacctgg ccactctgga ttctgtgcag ccgctcgatg atctgagcgc
     1981 cagcgtatac agcaactttg gtgtctccag ctcgttttcc ttcaagcctg gttcttagtg
     2041 aggatcctaa ctcgaggcga atttcttatg atttatgatt tttattatta aataagttat
     2101 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     2161 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
     2221 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt
     2281 cgctggaaat ctgctcgtca gtggtgctca cactgacgaa tcatgtacag atcataccga
     2341 tgactgcctg gcgactcaca actaagcaag acagccggaa ccagcgccgg cgaacaccac
     2401 tgcatatatg gcatatcaca acagtccacg tctcaagcag ttacagagat gttacgaacc
     2461 actagtgcac tgcagtacat cgaggagaac ttctagtata tctacatacc taatattatt
     2521 gccttattaa aaatggaatc ccaacaatta catcaaaatc cacattctct tcaaaatcaa
     2581 ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt atatttatag gataattata
     2641 ctctatttct caacaagtaa ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag
     2701 aaatatcttg accgcagtta actgtgggaa tactcaggta tcgtaagatg caagagttcg
     2761 aatctcttag caaccattat ttttttcctc aacataacga gaacacacag gggcgctatc
     2821 gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc gcctgacgca
     2881 tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagagcgc cggaaccggc
     2941 ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat acttgaagtt
     3001 gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat cgtcttactt
     3061 tctaactttt cttacctttt acatttcagc aatatatata tatatatttc aaggatatac
     3121 cattctaatg tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga
     3181 aatcacagcc gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa
     3241 gttcgatttc gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgttccact
     3301 tccagatgag gcgctggaag cctccaagaa ggctgatgcc gttttgttag gtgctgtggg
     3361 tggtcctaaa tggggaaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa
     3421 agaacttcaa ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga
     3481 cttatctcca atcaagccac aatttgctaa aggtactgac ttcgttgttg tcagagaatt
     3541 agtgggaggt atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga
     3601 tagtgaacaa tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc
     3661 cctacaacat gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc
     3721 ttcaagatta tggagaaaaa ctgtggagga aaccatcaag aacgaatttc ctacattgaa
     3781 ggttcaacat caattgattg attctgccgc catgatccta gttaagaacc caacccacct
     3841 aaatggtatt ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt
     3901 tatcccaggt tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa
     3961 caccgcattt ggtttgtacg aaccatgcca cggttctgct ccagatttgc caaagaataa
     4021 ggtcaaccct atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt
     4081 gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag gttttggatg caggtatcag
     4141 aactggtgat ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga
     4201 agttaagaaa atccttgctt aaaaagattc tcttttttta tgatatttgt acataaactt
     4261 tataaatgaa attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg
     4321 tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatgt
     4381 aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg
     4441 cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag gtgtaacaga
     4501 aaatcatgaa actatgattc ctaatttata tattggagga ttttctctaa aaaaaaaaaa
     4561 atacaacaaa taaaaaacac tcaatgacct gaccatttga tggagtttaa gtcaatacct
     4621 tcttgaacca tttcccataa tggtgaaagt tccctcaaga attttactct gtcagaaacg
     4681 gccttaacga cgtagtcgag agtactcgta tcgcatgtcg gtgcgacacg aaattacaaa
     4741 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
     4801 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
     4861 tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg gcaccacaca
     4921 aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat attggaggat
     4981 tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
     5041 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
     5101 ttttactctg tcagaaacgg ccttaacgac gtagtcgacc tcctcttcag tactaaatct
     5161 accaatacca aatctgatgg aagaatgggc taatgcatca tccttaccca gcgccgagcg
     5221 gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     5281 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     5341 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     5401 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     5461 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     5521 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     5581 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg
     5641 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     5701 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     5761 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     5821 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     5881 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     5941 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     6001 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
     6061 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     6121 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     6181 tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc ccttaacgtg
     6241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6961 ctgattctgt ggataaccgt gcggccgcca atacccagaa gaacagtaaa ataaagcaag
     7021 gtacgtgaaa ttaatatttt taaatggttc taaccgatgc cgaagaactg cgcagtccgg
     7081 ttataacgtc tgacatgtcc ttttttgatt tggaatccaa ccactcaagt gactctgttc
     7141 atttactttg cgaaaaatat acccacaaat tgcccatcga aagtgaatcg caaaccacct
     7201 tcagactggc accgacaaag caaagattat acagacagag tactttatac gtaccgttaa
     7261 gtctcaagca aagggttttc ttatttactg aacgggtaaa gagtatctgg gccggcttgc
     7321 caagatgcaa accgaataag tatttcaaag ttgcatttgc cttagccgtc ctgacaccat
     7381 tggctatttg gatattttat attgactttc gtgtacattg atcacatcga ctgttctatt
     7441 ggcaaatgaa ccacgggcat tgactatttt tcaggttact actatatatt atcatcacgg
     7501 gcaaggattg taccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag
     7561 ccggtacaag caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg
     7621 acggcgatca cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc
     7681 aatacaacg
//
