LOCUS       sequence-431979-        2195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2195)
  TITLE     MoClo-compatible AtU6-driven sgRNA array for plant genome 
            engineering
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pK6-K7
FEATURES             Location/Qualifiers
     source          1..2195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..75)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        143..247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             469..1260
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1431..2019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1920..1939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2173..2190
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcgtgg cggtggtaat gccatgtaat atgactcaga gaccgtggac gaaagggcct
       61 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      241 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
      301 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct
      361 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
      421 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
      481 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
      541 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
      601 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
      661 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
      721 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
      781 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
      841 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
      901 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
      961 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     1021 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     1081 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     1141 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     1201 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
     1261 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1321 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1381 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1441 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1501 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1561 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1621 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1681 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1741 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1801 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1861 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1921 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1981 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2041 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2101 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2161 acgaccgagc gcagcgagtc agtgagcgag gaagc
//
