LOCUS       sequence-431982-        2194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo
            Compatible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2194)
  TITLE     MoClo-compatible AtU6-driven sgRNA array for plant genome 
            engineering
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pK8-J11
FEATURES             Location/Qualifiers
     source          1..2194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..74)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        142..246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             468..1259
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1430..2018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1919..1938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2172..2189
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcgtgg ggcaatgtgc agcgaaacta agcgctagag accgtggacg aaagggcctc
       61 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      121 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      241 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      301 gcagtggcgg ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc
      361 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
      421 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
      481 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
      541 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
      601 acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
      661 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
      721 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
      781 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
      841 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
      901 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
      961 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     1021 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     1081 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     1141 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     1201 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
     1261 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1321 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1381 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1441 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1501 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1561 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1621 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1681 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1741 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1801 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1861 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1921 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1981 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2041 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2101 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2161 cgaccgagcg cagcgagtca gtgagcgagg aagc
//
