LOCUS       sequence-431983-        2198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2198)
  TITLE     MoClo-compatible AtU6-driven sgRNA array for plant genome 
            engineering
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pK4-K5
FEATURES             Location/Qualifiers
     source          1..2198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..93)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        161..265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             487..1278
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1449..2037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1938..1957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2191..2198,1..10)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agtgagcgag gaagcggtct cgccaccaat ttacatcttt atttattaac gttcgagaga
       61 ccgtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      121 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      181 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      241 tcaataatat tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa ccttgaccga
      301 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gttttttttt
      361 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      421 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
      481 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
      541 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
      601 gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt
      661 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
      721 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
      781 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
      841 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
      901 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
      961 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     1021 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     1081 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     1141 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     1201 ttggcctcgc gcgcagatca gttggaagaa tttgtccatt acgtaaaagg cgagatcacc
     1261 aaggtagtcg gcaaataact gtcagaccaa gtttactcat atatacttta gattgattta
     1321 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1381 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1441 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1501 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1561 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1621 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1681 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1741 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1801 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1861 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1921 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1981 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2041 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2101 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2161 accgctcgcc gcagccgaac gaccgagcgc agcgagtc
//
