LOCUS       sequence-431986-        2198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminates AtU6-promoter driven sgRNA array with four guides, MoClo 
            Compatible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2198)
  TITLE     MoClo-compatible AtU6-driven sgRNA array for plant genome 
            engineering
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pK4-J11
FEATURES             Location/Qualifiers
     source          1..2198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(60..78)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        146..250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             472..1263
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1434..2022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1923..1942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2176..2193
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcgcca ccaatttaca tctttattta ttaacgcgct agagaccgtg gacgaaaggg
       61 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      241 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      301 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
      361 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      421 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
      481 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
      541 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
      601 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
      661 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
      721 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
      781 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
      841 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
      901 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
      961 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1021 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1081 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1141 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     1201 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     1261 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1321 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1381 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1441 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1501 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1561 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1621 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1681 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1741 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1801 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1861 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1921 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1981 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2041 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2101 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2161 cgaacgaccg agcgcagcga gtcagtgagc gaggaagc
//
