LOCUS       sequence-431992-        2191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminates AtU6-promoter driven sgRNA array with two guides, MoClo 
            Compatible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2191)
  TITLE     MoClo-compatible AtU6-driven sgRNA array for plant genome 
            engineering
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pK2-J11
FEATURES             Location/Qualifiers
     source          1..2191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     277..296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     530..547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(605..623)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        691..795
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1017..1808
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1979..2191,1..376)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
       61 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      121 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
      181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      241 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      301 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      361 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      421 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      481 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      541 gagcgaggaa gcggtctcga agcctaggct gcacgggcat ccgctagaga ccgtggacga
      601 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      661 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      781 tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      841 ggtaacggcg cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt
      901 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      961 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
     1021 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1081 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1141 tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1201 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     1261 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1321 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1381 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1441 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1501 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1561 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1621 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1681 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1741 gcgcagatca gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg
     1801 gcaaataact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2161 agaactctgt agcaccgcct acatacctcg c
//
