LOCUS       sequence-431993-       12715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLRP3 tagged with mNeonGreen in mammalian cells 
            (lentiviral); driven by the weak pGK promoter with blast selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 12715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: pHAGE pGK hNLRP3-mNG
FEATURES             Location/Qualifiers
     source          1..12715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             2852..3085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3157..3745
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3263..3274
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3672..3692
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3746..4144)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      4141..4150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4169..4380)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    4488..4512
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4514..5221)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      4960..4969
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8365..8389)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8396..8895)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8444..8463)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8837..8860
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    8991..9108
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9406..9450)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9635..9868
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10365..10490
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10537..10717
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10735..10933)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10760..10780)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10935..11314
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11486..11505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11580..11684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11685..11753,11754..12545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11903..11922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     2881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     2941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3001 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     3061 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     3121 acagctgcct tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga
     3181 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     3241 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     3301 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     3361 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     3421 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     3481 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     3541 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     3601 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     3661 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     3721 aaattttgta atccagaggt tgattttagc cctcccacac ataaccagag ggcagcaatt
     3781 cacgaatccc aactgccgtc ggctgtccat cactgtcctt cactatggct ttgatcccag
     3841 gatgcagatc gagaagcacc tgtcggcacc gtccgcaggg gctcaagatg cccctgttct
     3901 catttccgat cgcgacgata caagtcaggt tgccagctgc cgcagcagca gcagtgccca
     3961 gcaccacgag ttctgcacaa ggtcccccag taaaatgata tacattgaca ccagtgaaga
     4021 tgcggccgtc gctagagaga gctgcgctgg cgacgctgta gtcttcagag atggggatgc
     4081 tgttgattgt agccgttgct ctttcaatga gggtggattc ttcttgagac aaaggcttgg
     4141 ccatggtggc agcgctctag aaccggtcct gtgttctggc ggcaaacccg ttgcgaaaaa
     4201 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     4261 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     4321 atcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     4381 actgacgggc accggagcca attcccactc ctttcaagac ctagctagtc ctgcaggttc
     4441 gctaccttag gaccgttata gttacgctcg aggtttaaac tcattaaacc actttgtaca
     4501 agaaagctgg gtcctacttg tacagctcgt ccatgcccat cacatcggta aaggcctttt
     4561 gccactcctt gaagttgagc tcggtcttgg agtgcttgag ctccgtctta cggaacacgt
     4621 acatcggctg gttcttcaga tagttagccg ccattggctt ggcaaaggtg taggtggtcc
     4681 gcgcagtgct ccggtagcgc ttgccatttc cagtggtgta actccactta aaggtactga
     4741 tgatggtttt gtcgttgggg taagtcttct tcgacctgca ccagtccgca gcggtcagcg
     4801 agttggtcat cacaggaccg tcagcaggga aaccagtccc cttcacctgg gcctctcctt
     4861 tgatgtggct tccctcgtag gtgtagcggt agttaacagt aagggaggca ccatcttcaa
     4921 actgcattgt gcgatggact tggtatccgg agccatctac catggcggcc tggaaaggcg
     4981 acatcccgtc agggtagggc aggtactgat ggaagccata cccgatatga gggaccagaa
     5041 tccaggggga gaactggagg tcacccttgg tggacttcag gtttaactcc tcataaccat
     5101 catttggatt gccggtgccc tgacccacca tgtcaaagtc cacaccgttg atggagccaa
     5161 agatgtgtaa ctcatgtgtc gctgggagag aggccatgtt atcctcctcg cccttgctca
     5221 cggatccgcc gcttcccccg gatccgccca accaagaagg ctcaaagacg acggtcagct
     5281 caggcttttc ttcttgaagt gtttctaacg cactttttgt ctcataattg aaatacattt
     5341 cagacaaccc caggttctgc aggaggcagc tctgctgttt cagcacttca cagaacatca
     5401 tgacccccag gtcgcccagg tcattgttgc ccaggctcag ctttcgcagg ctctggctgg
     5461 aggtcagaag tgtggaaaga tcccagcagc agtgtgacgt gaggttgcag ttgtctaatt
     5521 ccaacacctg aagcttgcag tcggggtgca agagtccctc acagagtagt ttgatcccct
     5581 tgtctccgag agtgttgcct cgcaggtaaa ggtgcgtgag attctgatta gtgctgagta
     5641 ccgaggacaa agctgaacaa cagactgacg taaggccaga attcaccaac cccagtttct
     5701 gcaggttaca ctgtggattc ttggcttttt cacataaaat tgcgactcct gagtctccca
     5761 aggcattctc ccccacatag agtctggtca gggaatggct ggtgctcaat actgatgcaa
     5821 gatcctgaca acatgctgat gtgaggcagc agctgaccaa ccagagcttc ttcagattgc
     5881 acaacaggtg cttcagtccc acacacagaa gtctgattcc gaagtcaccg agggcgttgt
     5941 cactcaggtc cagctccacc agcttctggt tgctgctgag gaccaaggag atgtcgaagc
     6001 agcactcatg cgagaggcca cagcgcccca accacaatct ccgaatgtta cagccaggat
     6061 gctggagcgt ttcacacaac actctcatcc ctgggtcccc cagagaattg tcactgaggt
     6121 ccaattcagt tagactctgg ctggtgctca gaactgaaaa gaggccccgg caaaaactgg
     6181 aagtgaggtg gctgttcacc aatccatgag aacaggcagc atgagaggag cttgggagga
     6241 cacactgcac catatcaagg tgtcggcctt ccttttcctc ctcctcttcc tccttgggca
     6301 tgttatggag aaaccccagg gacagtgact ccacccgatg acagttctca atgcaaaagg
     6361 aagaaaccat gtggtccatt ctggtggaga gattgatctc aatcttgggg aaatagtcca
     6421 tggccctttg cacgaagtcc tcctcctgca tctcgtacaa acagtagaac aattccagct
     6481 ggctgggctg gatctgcagc tttttagctt tggctttcac ttcaatccat ttcagcagct
     6541 ccagcctgat ttgctgagag atcttgcaac ttaatttctt ctccaagtag gaggtcctct
     6601 cctggtttac caggccaaag aggaaacgta caacaaaaat caaatacccc ttttcgaatt
     6661 tgccatagtt ttccagaagg actgtcacgt ctcggctggg aagcttcaaa cgactccctg
     6721 gaacgttcgt ccttccttcc ttttcctctt ccagcaggta gtacatggcg gcaaagaact
     6781 cctggaaagt catgtggatg aagctgtaga acttctcgca gtccacttcc ttttggaaca
     6841 ggttcatcct caggaaagca gacacatccg ccttctgcag tccatgattc ctgaggtcgg
     6901 actcctcaaa caggattttc tggttccaga ttccatctgc agccaaagag cagagccccc
     6961 agaggtgggc gcagaggccg tgctcctggc tccctccccg gggctgcagc aaactggaaa
     7021 ggaagaagac gtacaccgcg gtggtggtct tggatgtctg ggcaaggctc ttgccactct
     7081 ccatctgctg tttcagtcca gtgcacacga tccagcagac cagggggatg aagcacatgg
     7141 tgaagaggac ctcgttctcc tgaatcagac tgaaggctgc cctggcttgg gcctcatcag
     7201 agaagtactt gaagaagtac tctttccttt tggcctcgga gaaacccagg atctccacat
     7261 gccgaggatg gtccagcaag tgctgcagtt tctccagggc cacaggtctc gtggtgatga
     7321 gcagagaggc ctcgggaagc agcttctttc tgatgaggct gctcaggaga atgtctcccc
     7381 gctcggcctt ctgccagtca gtgcagagcg gtcctatgtg ctcgtcaaag gcaccttgca
     7441 gctcatcgaa gccgtccatg aggaagagga ttctggaggg ttttctcacg atcttgtgga
     7501 tgggtgggtt tgggtcgggg cagcagctca tgatcaggtc ccccaggctc ctctgtgtca
     7561 caaggctcac ctcccgacag tggatataga acagatagtc aaacctgtct tggtagagtg
     7621 tccccgacgc ccagtccaac atcatcttcc tggccaggat tgttttccca atccctgccg
     7681 ccccctggaa caccacggtg tgcacaggct cagaatgctc atcatcgggg tcaaacagca
     7741 actccatctt aatgggactc acggggctct cacacgtctt ggtcttgccg atggccagaa
     7801 gctcctgctc cctctcctgc tggctccggt gctccttgat gagacgcagt cgtgtgtagc
     7861 gtttgttgag gctcacactc tcacccagac gggcattcct gtcttcaatg cactggaatc
     7921 tgcttctcac gtactttctg tacttcttac ggtaatcttt cttcatttta caaatagaga
     7981 ttctcgaaag gtactccagt aaacccatcc actcctcttc aatgctgtct tcctggcata
     8041 tcacagtggg attcgaaaca cgtgcattat ctgaacccca cttcggctca tctctttttg
     8101 ctttctcata aaggtctctc ctgttgatcg cagcgaagat ccacacggcc atggcccacg
     8161 ccttctcctc cccattgaag tcgatcatta gcgtggctag atccacatgg tctgccttct
     8221 ctgtctgacc cctcgggagg gggatgcagc ccttctgggg aggatagtcc tctaagtgca
     8281 tcttaaattt cttcaagtcc acatcctcca ggtcctccag gtacctggcc agcttgcagc
     8341 gggtgcttgc catcttcatg gtggagcctg cttttttgta caaacttgtt tcgaacgaaa
     8401 ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga
     8461 atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc
     8521 cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct
     8581 attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca
     8641 cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg
     8701 cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga
     8761 acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg
     8821 tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa
     8881 gcgcctcccc tacccatagg gataacaggg taatataggc ggccgcgtag atggcgcgcc
     8941 ggtaccttaa ttaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
     9001 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     9061 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     9121 actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga
     9181 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct
     9241 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     9301 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     9361 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     9421 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     9481 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     9541 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     9601 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     9661 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     9721 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     9781 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     9841 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     9901 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     9961 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
    10021 ccggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
    10081 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
    10141 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
    10201 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
    10261 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
    10321 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
    10381 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
    10441 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
    10501 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
    10561 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
    10621 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
    10681 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt
    10741 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
    10801 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
    10861 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
    10921 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
    10981 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
    11041 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
    11101 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
    11161 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
    11221 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
    11281 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
    11341 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
    11401 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
    11461 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11521 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
    11581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    11641 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11701 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11761 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11821 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11881 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11941 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    12001 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    12061 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    12121 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    12181 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    12241 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    12301 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    12361 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    12421 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    12481 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    12541 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    12601 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12661 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
