LOCUS       sequence-432008-        9102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expressing an RNA containing your sequence of interest adjacent 
            to two MS2 sites from an RNA polymerase III promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Bernstein DS, Buter N, Stumpf C, Wickens M
  TITLE     Analyzing mRNA-protein complexes using a yeast three-hybrid system.
  JOURNAL   Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4.
  PUBMED    12054889
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Methods"; date: "2002-02"; volume: "26"; issue: "2"; pages: 
            "123-41"
COMMENT     Sequence Label: pIIIA/MS2-2
FEATURES             Location/Qualifiers
     source          1..9102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        196..416
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             417..1220
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1488..1509)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1700..1719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1936..1958
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1950..1967
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1951..1967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1977..1996
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1977..1995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2065..2440
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             2441..4156
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     terminator      complement(4822..4884)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(4917..4935)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(4956..4974)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(5039..5445)
                     /gene="RPR1"
                     /label=RPR1 promoter
                     /note="promoter for the S. cerevisiae RNase P RNA gene"
     primer_bind     complement(5447..5463)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5500..5520)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5500..5518)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5539..5555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5539..5555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5552..5574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5563..5579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5587..5593,5594..5611,5612..5617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5770..5787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5941..6529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6021..6040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6700..7491,7492..7560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7323..7342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7561..7665)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7692..9034
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8655..8702)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9075..9093
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgactctaga ggatccccgg
     2041 ccgggggatc tcggaattga ctttttcttg aataatacat aacttttctt aaaagaatca
     2101 aagacagata aaatttaaga gatattaaat attagtgaga agccgagaat tttgtaacac
     2161 caacataaca ctgacatctt taacaacttt taattatgat acatttctta cgtcatgatt
     2221 gattattaca gctatgctga caaatgactc ttgttgcatg gctacgaacc gggtaatact
     2281 aagtgattga ctcttgctga ccttttatta agaactaaat ggacaatatt atggagcatt
     2341 tcatgtataa attggtgcgt aaaatcgttg gatctctctt ctaagtacat cctactataa
     2401 caatcaagaa aaacaagaaa atcggacaaa acaatcaagt atggattcta gaacagttgg
     2461 tatattagga gggggacaat tgggacgtat gattgttgag gcagcaaaca ggctcaacat
     2521 taagacggta atactagatg ctgaaaattc tcctgccaaa caaataagca actccaatga
     2581 ccacgttaat ggctcctttt ccaatcctct tgatatcgaa aaactagctg aaaaatgtga
     2641 tgtgctaacg attgagattg agcatgttga tgttcctaca ctaaagaatc ttcaagtaaa
     2701 acatcccaaa ttaaaaattt acccttctcc agaaacaatc agattgatac aagacaaata
     2761 tattcaaaaa gagcatttaa tcaaaaatgg tatagcagtt acccaaagtg ttcctgtgga
     2821 acaagccagt gagacgtccc tattgaatgt tggaagagat ttgggttttc cattcgtctt
     2881 gaagtcgagg actttggcat acgatggaag aggtaacttc gttgtaaaga ataaggaaat
     2941 gattccggaa gctttggaag tactgaagga tcgtcctttg tacgccgaaa aatgggcacc
     3001 atttactaaa gaattagcag tcatgattgt gaggtctgtt aacggtttag tgttttctta
     3061 cccaattgta gagactatcc acaaggacaa tatttgtgac ttatgttatg cgcctgctag
     3121 agttccggac tccgttcaac ttaaggcgaa gttgttggca gaaaatgcaa tcaaatcttt
     3181 tcccggttgt ggtatatttg gtgtggaaat gttctattta gaaacagggg aattgcttat
     3241 taacgaaatt gccccaaggc ctcacaactc tggacattat accattgatg cttgcgtcac
     3301 ttctcaattt gaagctcatt tgagatcaat attggatttg ccaatgccaa agaatttcac
     3361 atctttctcc accattacaa cgaacgccat tatgctaaat gttcttggag acaaacatac
     3421 aaaagataaa gagctagaaa cttgcgaaag agcattggcg actccaggtt cctcagtgta
     3481 cttatatgga aaagagtcta gacctaacag aaaagtaggt cacataaata ttattgcctc
     3541 cagtatggcg gaatgtgaac aaaggctgaa ctacattaca ggtagaactg atattccaat
     3601 caaaatctct gtcgctcaaa agttggactt ggaagcaatg gtcaaaccat tggttggaat
     3661 catcatggga tcagactctg acttgccggt aatgtctgcc gcatgtgcgg ttttaaaaga
     3721 ttttggcgtt ccatttgaag tgacaatagt ctctgctcat agaactccac ataggatgtc
     3781 agcatatgct atttccgcaa gcaagcgtgg aattaaaaca attatcgctg gagctggtgg
     3841 ggctgctcac ttgccaggta tggtggctgc aatgacacca cttcctgtca tcggtgtgcc
     3901 cgtaaaaggt tcttgtctag atggagtaga ttctttacat tcaattgtgc aaatgcctag
     3961 aggtgttcca gtagctaccg tcgctattaa taatagtacg aacgctgcgc tgttggctgt
     4021 cagactgctt ggcgcttatg attcaagtta tacaacgaaa atggaacagt ttttattaaa
     4081 gcaagaagaa gaagttcttg tcaaagcaca aaagttagaa actgtcggtt acgaagctta
     4141 tctagaaaac aagtaatata taagtttatt gatatacttg tacagcaaat aattataaaa
     4201 tgatatacct attttttagg ctttgttatg attacatcaa atgtggactt catacataga
     4261 aatcaacgct tacaggtgtc cttttttaag aatttcatac ataagatcca ctagcctctg
     4321 caaagcttca tggcctttat aaaaaggaac tatccaatac ctcgccagaa ccaagtaaca
     4381 gtattttacg gggcacaaat caagaacaat aagacaggac tgtaaagatg gacgcattga
     4441 actccgaaag aacaacaaga gttccaaaaa gtagtggaac aaaagcaaat gagggatttc
     4501 atgcgtttgt actctaatct ggtagaaaga tgtttcacag actgtgtcaa tgacttcaca
     4561 acatcaaagc taaccaataa ggaacaaaca tgcatcatga agtgctcaga aaagttcttg
     4621 aagcatagcg aacgtgtagg gcagcgtttc caagaacaaa acgctgcctt gggacaaggc
     4681 ttgggccgat aaggtgtact ggcgtatata tatctaatta tgtatctctg gtgtagccca
     4741 tttttagcat gtaaatataa agagaaacca tatctaatct aaccaaatcc aaacaaaatt
     4801 caatagttac tatcgctttt ttctttctgt atcgcaaata agtgaaaatt aaaaaagaaa
     4861 gattaaattg gaagttggat atgggggaat tccgatcctc tagagtcgac ctgcagacat
     4921 gggtgatcct catgttttct agagtcgacc tgcagacatg ggtgatcctc atgttttcta
     4981 gagtcgacct gcaggcatgc aagctgcccg ggggatccac tagttctagc cggaattctg
     5041 ccaatcgcag ctcccagagt ttcgttccca gccgcgagca ccacagcgta ccatgtgcgc
     5101 cacgaggcct caaacgtaaa acaatcgaaa cgaaaagaaa cagactatag gggagtatag
     5161 agacagccgg ccaataagaa gaggaaaaag aaatactagc gtttatcaat gtgggtcgtt
     5221 aacatatcct gttgacaatg attacaggtt aaaaggtagc gtaagtgaat attaactatg
     5281 gatattctta tacttagata agagacatat aaaacacacg atgattgatt gatttttatg
     5341 accaatatat gtaattcgta attcagatag tttttatact tttaatgtgt gccgaaccat
     5401 attactagta tatgtaacta ccatgttatg ttcaattggc agatcggatc cactagttct
     5461 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     5521 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5581 tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5641 gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6361 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7681 agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     7741 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     7801 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     7861 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     7921 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     7981 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     8041 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     8101 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     8161 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     8221 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     8281 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     8341 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     8401 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     8461 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     8521 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     8581 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt
     8641 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     8701 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     8761 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     8821 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     8881 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     8941 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     9001 ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga
     9061 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
