LOCUS       sequence-43208-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Nikkila H, Gennis RB, Sligar SG
  TITLE     Cloning and expression of the gene encoding the soluble cytochrome 
            b562 of Escherichia coli.
  JOURNAL   Eur J Biochem. 1991 Dec 5;202(2):309-13.
  PUBMED    1761034
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Biochem."; date: "1991-12-5"; volume: "202(2)"; pages: "309-13"
COMMENT     Sequence Label: pNS207
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(614..630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(614..630)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(627..649)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    638..654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(662..668,669..686,687..692))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    707..728
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc ggcagcagca
       61 atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca ggcaacaggg
      121 aaatgaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc agttgctctg
      181 cagcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg tcgtcaatct
      241 gaccgaccag aatgtcgaaa ccgtggcgga aatctttcat ttccgggctg tccggtgatt
      301 tatcttcgag cttcggcggc gttgcttttt gcgcatccag cgctgcggcg cgcatcttcg
      361 ttaacgcgtc tttgacttgc gccgcgttat ccgctttttc gatcactttt aaattgtcgt
      421 tgagggtttc catattgtct tcaagatcag cggcaaacga cgcagaactg aataccaacg
      481 aggagactgc aagaatagct aacaggcttt tacgcattgc tcacttcctt ttttatttac
      541 acttcatcct tcacactgcc tcatcgacgg ctacgctcat tcgcccgggt accgagctcg
      601 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      661 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      721 tcacattaat tgcgttgcgc tcactgcccg c
//
