LOCUS       sequence-432117-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 [Sp] CRISPRi targeting CD47, positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller 
            NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, 
            Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG
  TITLE     Modular vector assembly enables rapid assessment of emerging CRISPR 
            technologies.
  JOURNAL   Cell Genom. 2024 Mar 13;4(3):100519. doi: 
            10.1016/j.xgen.2024.100519.
  PUBMED    38484704
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2024-03-13"; volume: "4(3):100519. doi"; pages: " 
            10.1016/j.xgen.2024.100519"
COMMENT     Sequence Label: pRDB_162
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    569..694
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             741..921
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(939..1137)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(964..984)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1139..1518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1690..1709
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1784..1888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1889..1957,1958..2749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2107..2126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2920..3508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3409..3428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3662..3679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3796..3817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3832..3837,3838..3855,3856..3862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3870..3886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3875..3897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3894..3910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3894..3910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3959..4080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3970..3989)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4024..4043
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4210..4584)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4603..4650)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      4712..4846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4766..4785)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4991..5011
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5020..5448)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5130..5151)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5342..5361
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5494..5718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5707..5724
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5750..5983
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    6055..6643
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6161..6172
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6570..6590
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6679..7275)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6759..6779)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7256..7275
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7286..8464)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7295..8233
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7318..7338)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(8584..8832)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8642..8661)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8812..8832)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    8890..9007
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9305..9349)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9534..9691,1..76)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
       61 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
      121 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
      181 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg
      241 cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt
      301 ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa
      361 gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt
      421 ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata
      481 ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc
      541 gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc
      601 ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc
      661 gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca
      721 agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga
      781 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
      841 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
      901 ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag acctcccacc
      961 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     1021 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     1081 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     1141 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     1201 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     1261 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     1321 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     1381 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     1441 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     1501 tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac
     1561 gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt
     1621 aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc
     1681 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc
     1741 tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1801 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1861 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1921 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1981 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2041 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2101 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2161 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2221 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2281 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2341 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2401 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2461 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2521 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2581 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2641 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2701 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2761 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3541 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3601 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3661 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3721 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3781 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3841 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3901 cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata
     3961 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     4021 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4081 ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     4141 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     4201 tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg
     4261 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
     4321 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
     4381 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
     4441 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     4501 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     4561 aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt atactatgcc
     4621 gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa aatggatata
     4681 caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     4741 tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     4801 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggactat ggttgctgac
     4861 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     4921 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     4981 gactttccac accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt
     5041 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5101 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5161 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5221 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5281 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5341 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5401 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc
     5461 tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
     5521 gcctgctatt atcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     5581 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     5641 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     5701 ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct gctagagatt
     5761 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     5821 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     5881 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
     5941 aaaagcagat cttatcttcg ttgggagtga attagccctt ccagtccccc cttttctttt
     6001 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagt cgacgcgggg
     6061 aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaggacgc ggaagaggcc
     6121 gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt
     6181 agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg
     6241 acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc
     6301 agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga
     6361 aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag
     6421 caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag
     6481 acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat
     6541 gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa
     6601 taccagtcaa tctttcacaa attttgtaat ccagaggttg attttcgaat gcatcgtaac
     6661 tgagtcctat tagcttccgg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     6721 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     6781 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     6841 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagactc cgacggtggc
     6901 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     6961 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     7021 ccccgcttcg acgctctccg gcgtggtcca gaccgccacg gcggcgccgt cgtccgcgac
     7081 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     7141 ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     7201 ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     7261 cttgtactcg gtcatggtgg ctccttcacg acacctgaaa tggaagaaaa aaactttgaa
     7321 ccactgtctg aggcttgaga atgaaccaag atccaaactc aaaaagggca aattccaagg
     7381 agaattacat caagtgccaa gctggcctaa cttcagtctc cacccactca gtgtggggaa
     7441 actccatcgc ataaaacccc tccccccaac ctaaagacga cgtactccaa aagctcgaga
     7501 actaatcgag gtgcctggac ggcgcccggt actccgtgga gtcacatgaa gcgacggctg
     7561 aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc
     7621 gcgtcctcca ttttgagctc cctgcagcag ggccgggaag cggccatctt tccgctcacg
     7681 caactggtgc cgaccgggcc agccttgccg cccagggcgg ggcgatacac ggcggcgcga
     7741 ggccaggcac cagagcaggc cggccagctt gagactaccc ccgtccgatt ctcggtggcc
     7801 gcgctcgcag gccccgcctc gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc
     7861 ccgcggcccc aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt acaagactat
     7921 cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat
     7981 cgaaagcagc gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc
     8041 caggccaggc ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac
     8101 tctcccaccc acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggtgg
     8161 aagtaattca aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac
     8221 acacggcact tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga
     8281 ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga
     8341 catcactttc ccagtttacc ccgcgccacc ttctctaggc accggatcaa ttgccgaccc
     8401 ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg
     8461 agccgtgttc aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
     8521 ttatttaaac ttgctatgct gtttccagca tagctcttaa actggtagcg gcgctgttgc
     8581 tgcggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
     8641 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
     8701 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
     8761 aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
     8821 aaataggccc tctctgcccg gtaccttaat taaccaaact ggatctctgc tgtccctgta
     8881 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     8941 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     9001 tttaaaattg tggatgaata ctgccatttg tctgcagaat tggcgcacgc agtgccgatc
     9061 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt
     9121 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
     9181 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc
     9241 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
     9301 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
     9361 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc
     9421 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
     9481 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
     9541 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
     9601 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct
     9661 gctgcactat accagacaat aattgtctgg c
//
