LOCUS       sequence-43214-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     14-3-3 plasmids
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1479 pcDNA3 flag HA 14-3-3 gamma
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttaccatg gactacaagg acgacgatga caagctcgat
      121 ggaggatacc cctacgacgt gcccgactac gccagcggat ccccggaatt catggtggac
      181 cgcgagcaac tagtgcagaa agcccggctg gccgagcagg cggagcgcta cgacgatatg
      241 gccgcggcca tgaagaacgt gacagagctg aatgaaccac tgtccaacga ggaacggaac
      301 ctcctgtcgg tggcctacaa gaacgtggtt ggggctcgcc gctcctcctg gagggtcatc
      361 agcagcatcg agcagaagac gtctgcggat ggcaacgaga agaagataga gatggtccga
      421 gcctaccggg aaaagattga gaaggagctg gaggccgtgt gccaggacgt gctgagcctg
      481 ctggacaact acctgatcaa gaactgcagc gagacccagt acgagagcaa ggtgttctac
      541 ctgaagatga aaggggacta ttaccgctac ctggctgaag tggccactgg ggagaaaagg
      601 gcgaccgtgg tggagtcgtc cgagaaggcc tacagcgaag cccacgagat cagcaaggag
      661 cacatgcagc ccacccaccc catccggctg ggcctggcgc tcaactactc cgttttctac
      721 tacgagatcc agaacgcccc agagcaagcc tgccacctgg ccaagaccgc cttcgatgac
      781 gccatcgccg agctcgacac tctgaacgag gactcctaca aggactccac tctgatcatg
      841 cagctgctcc gagacaacct aac
//
