LOCUS       sequence-43215-e        2694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O'Hanlon D, 
            Sung HK, Alvarez M, Talukder S, Pan Q, Mazzoni EO, Nedelec S, 
            Wichterle H, Woltjen K, Hughes TR, Zandstra PW, Nagy A, Wrana JL, 
            Blencowe BJ
  TITLE     An alternative splicing switch regulates embryonic stem cell 
            pluripotency and reprogramming.
  JOURNAL   Cell. 2011 Sep 30;147(1):132-46. Epub 2011 Sep 15.
  PUBMED    21924763
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-30"; volume: "147(1)"; pages: "132-46. Epub 2011 Sep 
            15"
COMMENT     Sequence Label: pCMV10-mFoxP1-ES
FEATURES             Location/Qualifiers
     source          1..2694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        147..350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     301..321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             380..445
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 actgcagtac atcaagtgta tcatatgcca agtccgcccc tatgacgtca atgacggtaa
       61 tggcccgctg gcatatgccc agtacatgac cttacgggac ttctacttgg cagtacatct
      121 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      181 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      241 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      301 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      361 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      421 cgattacaag gatgacgatg acaagcttgc ggccgcgatg caagaatctg ggtctgagac
      481 aaaaagtaac ggatcagcca tccagaacgg gtccagcggt ggcaaccact tactagagtg
      541 cggggcactt cgtgacactc ggtccaacgg agaggcacca gcggtggacc tgggggcagc
      601 cgaccttgcc cacgtccagc agcagcaaca gcaggccctg caggtggcaa ggcagctcct
      661 ccttcagcag cagcaacagc agcagcagca acagcagcag cagcagcagc agcaacagca
      721 gcaacagcag cagcaacaac agcagcagca gcaacagcag cagcagcagc agcaagttag
      781 tggattaaag tctcccaaga ggaatgacaa gcaaccagct cttcaggttc ccgtgtcagt
      841 ggctatgatg acacctcagg ttatcactcc tcaacaaatg cagcagatcc tccagcagca
      901 ggtgctgagc ccccagcagc tccaggttct cctccagcag cagcaggccc tcatgcttca
      961 acagcagcag cttcaagaat tttataaaaa acaacaggaa cagttgcagc ttcaacttct
     1021 ccaacagcaa catgctggaa aacagccgaa agagcagcag caggtggcta cccagcagtt
     1081 ggctttccag cagcagcttc tccagatgca gcagctgcag cagcagcacc tcttgtctct
     1141 ccagcgccaa ggcctcctaa caattcagcc ggggcagcct gcccttcccc tccaacccct
     1201 cgctcaaggc atgattccaa cggaactgca gcagctctgg aaagaagtga caagtgccca
     1261 cactgcagag gaaaccacaa gcagcaacca cagcagccta gacctgacca gcacatgtgt
     1321 ctcgtcctcg gcaccttcca agtcctccct aatcatgaac ccgcatgcct ctaccaatgg
     1381 acagctctcg gtccacactc ccaaaaggga aagcttgtcc cacgaggagc acccccacag
     1441 ccaccctctc tatggacatg gcgtatgcaa gtggccaggc tgtgaggcgg tttgtgacga
     1501 cttcccagcc tttctaaaac atctcaacag tgagcatgcg ctggacgata gaagcacagc
     1561 tcaatgtaga gtacaaatgc aggttgtaca gcagttagag ctacagcttg caaaagacaa
     1621 agagcgcctg caagccatga tgacccacct gcatgtgaag tctacagaac ccaaagctgc
     1681 ccctcagccc ctgaatctgg tatcaagtgt caccctctcc aagtctgcct cagaggcttc
     1741 tccacagagc ttacctcata ctccaacaac ccccaccgcc cccctgactc ctgtcaccca
     1801 aggcccctcc gtcatcacca ccaccagcat gcacacggtg ggacctatcc gcaggcggta
     1861 ctcagacaaa tacaacgtgc ccatttcttc agcagatatt gcgcagaacc aagaatttta
     1921 taagaacgcg gaagttagac caccatttac atatgcatct ttaatcaggc aggccattct
     1981 cgaatctcca gaaaagcagc taacactaaa cgaaatctat aactggttca cacgaatgtt
     2041 tgcttacttc cgacgcaatg cagccacgtg gaagggtgcc attcgcacca acctctcact
     2101 gcataagtgc tttatcagag tagaggatga gtttgggtcc ttttggacag ttgatgatga
     2161 agagtttata cgtggccgcc atattcaacg aggccgtcct cgcaaatact gccccggtga
     2221 aaactctgac gagcttggcg cacataaccc ttcccttatt aaaaacatgc agagcagcca
     2281 cgcctactgc acacctctca atgcagcttt acaggcttcc atggctgaga atagtatacc
     2341 tctgtacact accgcttcca tgggaaatcc cactctgggc agcctggcca gtgccatccg
     2401 ggaggagctg aacggggcca tggagcacac caacagcaac gagagtgaca gcagtccagg
     2461 cagatcccct atgcaagctg tgcaccccat acacgtcaaa gaagaacccc tcgaccccga
     2521 ggaagctgaa ggccctctgt ccttagtgac aacagccaac cacagtccag attttgacca
     2581 tgacagagat tacgaagacg aaccagtaaa tgaggacatg gagtgaggat cccgggtggc
     2641 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccag
//
