LOCUS       sequence-432152-        7783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of human 20S CP proteasome subunit alpha 
            type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and 
            alpha type-4 (PSMA4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, 
            Harper JW, Hanna J, Schulman BA
  TITLE     Visualizing chaperone-mediated multistep assembly of the human 20S 
            proteasome.
  JOURNAL   Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
  PUBMED    38600324
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9."
COMMENT     Sequence Label: pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4
FEATURES             Location/Qualifiers
     source          1..7783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1052..1143)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1299..1433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2371..2462)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    2618..2752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3777..3868)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    3945..3978
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(4049..4054,4055..4071,4072..4077)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4247..4780
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4847..5070
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(5205..5793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5285..5304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5983..6148
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    6300..6434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7453..7544)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    join(7700..7783,1..51)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
       61 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
      121 taaaacctct acaaatgtgg tatggctgat tatgatcctc tagtacttct cgacaagctt
      181 gtcgagactg caggctctag attcgaaagc ggccgctcat tacatattat catcatctga
      241 ttcatcttct tccttcagag attccttagc atatttctct gcttcttctc ttatatcttt
      301 tggaacaatt tcatgtcttc cattagttaa ttcaccaacc cagctgagtt ctagttcaaa
      361 agctttatcc ttaacttcgt catgtactat gtaaattatt tttgcaactt ctttaacgat
      421 atcacggcag gtcatttctt tcatctgaag cttctctatt tccgtctttg cagcttgcct
      481 ggctttgccg atggcacagc cccaataacc gtatgaaaca cctgatgggt caatcatgta
      541 gagttgcgca ccgtcattca cactgtaaga ccctaacatg aaactgcagc caaaaggtct
      601 aacagcactg tagagtgtat atgcatgcac atacatggcc actctgtctg caagatgttt
      661 tagtggaatg ttgtagccaa agttagatct gaagttggaa gcttcttctc ttgctatgtc
      721 tgctaaagaa cgagcatctg ccaacaaacc tgctactgcc attccaacat gccgatcaac
      781 attaaaaagt cttttgttgg aaccttcttc ataaagttta gaaaggacta atttttctac
      841 cccaaagaca acaccatctt tgcatctgat tccaatagct gtactactat tttccacagc
      901 cttcatagca tattcaactt gaaaaactct tccgtcagga gagaatgtag aggctgacag
      961 gtcataccca gtgccgattg agctcatgaa ttccgcgcgc ttcggaccgg gatccgcgcc
     1021 cgatggtggg acggtatgaa taatccggaa tatttatagg tttttttatt acaaaactgt
     1081 tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa ttatctccat
     1141 gatctattaa tattccggag taggtcgatt taaacatcag gcatcattag gtttatttaa
     1201 atggttatga tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
     1261 tgttagcagc cggatcttct aggctcaagc agtgatcaga tccagacatg ataagataca
     1321 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1381 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1441 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1501 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc
     1561 ttgtcgagac tgcaggctct agattcgaaa gcggccgctc attatgctat ggcagccaag
     1621 taatccttaa cttcagttgg agtaagcctc ctaaatccag cttcattgca gattccaact
     1681 tctatgttat cctctgtcat ttgcccttca aagctttcct ttagggttaa gatggctgta
     1741 tgaatggcat cttcaagttc cagatcttca ttatatcttt tctcaaggaa agtcttccca
     1801 ttcacatagt tctttcccat tgctgtagct ttccaggcaa agtaagctcc agatggatct
     1861 gactgaaata aatatggtcg tccctcattc caaccacaaa taagtaaaga aactccaaat
     1921 ggacgaacac cacctgactg agtatattct tgcatcacag aagctactct ctgtaccagc
     1981 tgagctgtag gaatgggttc ttggtacaca agatagtatt gttgagctag ttttcgagct
     2041 ctgtgcacaa gcactctgta atcggggccc atgccactgt acaccaaacc tatatgcttg
     2101 gtaattggtt ctactttgtg tacacttcgc tcatcataca gaatggattt ctgttttttc
     2161 tcagttgcta ataccacacc atttgcagct ttaattccca cggacggggc tcctccagct
     2221 acagcagcca aagcatattc aatctggaca agtttaccag acgggctgaa tgtagtcagc
     2281 gaaaagctgt acccgcgctc cgccatgaat tccgcgcgct tcggaccggg atccgcgccc
     2341 gatggtggga cggtatgaat aatccggaat atttataggt ttttttatta caaaactgtt
     2401 acgaaaacag taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg
     2461 atctattaat attccggagt aggtcgattt aaacgtgcaa tagtatccag tttatttaaa
     2521 tggttatgat agttattgct cagcggtggc agcagccaac tcagcttcct ttcgggcttt
     2581 gttagcagcc ggatcttcta ggctcaagca gtgatcagat ccagacatga taagatacat
     2641 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2701 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2761 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2821 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagtacttc tcgacaagct
     2881 tgtcgagact gcaggctcta gattcgaaag cggccgctca ttaatgttcc attggttcat
     2941 cagccttttc tgcaggttca tcagcaggtt gagcaggctg tgcctttctc tgtggtcttt
     3001 cttcaagacc ttccaggaat ggagacacat catcatcatc atagattgta aactccaagt
     3061 ctttaccaac aattccaatg gaaacattct ttgtagtcag gtcctgttct gcaggaagcg
     3121 tctctcttaa ggcacgcaga ccatgtttaa ctagttcatt taaattacac tccataaatt
     3181 cagacatatg tctctccaag taagtacgag ctgattggga acgggctcca atggacatgg
     3241 ctctgcagtc aaaatagtta gcagatggac aggtttggaa aatgtgaggg cccatatcat
     3301 cataaccagc aataaggaga ccaacaccat atggtctccg gccatatcgt tgtgttggta
     3361 tctgggtctt gcttccaatt agagatacaa gacgagacac aggcagtggt ctatcgaata
     3421 caaatctgga atccaaacac tcctgacgca taaaattaca taacagtcta gcatcagcag
     3481 taagccccgc aattgagata ccaatatggt tgtcaacatg gagaattttt ttctgatgag
     3541 ctgcaagctc tgattgcgcc cttttcaatg caaccaaaac tgcatgagtt tttgatttca
     3601 gaccaactgt ggctgaacct tgtttaacag cttccattgc atattcaatt tgatgaatcc
     3661 tgccctgggg gctccaaaca gtgacatcat tgtcatactg atttcgaaac atgaattccg
     3721 cgcgcttcgg accgggatcc gcgcccgatg gtgggacggt atgaataatc cggaatattt
     3781 ataggttttt ttattacaaa actgttacga aaacagtaaa atacttattt atttgcgaga
     3841 tggttatcat tttaattatc tccatgatct attaatattc cggagtaggt cgcgaatcga
     3901 tactagtgtt taaactcgct accttaggac cgttatagtt acagataact tcgtataatg
     3961 tatgctatac gaagttatgg cgacttcgct gctgcccaag gttgccgggt gacgcacacc
     4021 gtggaaacgg atgaaggcac gaacccagtt gacataagcc tgttcggttc gtaaactgta
     4081 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     4141 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc
     4201 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     4261 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg
     4321 catcattcgc acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga
     4381 tcttttcggt cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga
     4441 ttacctcggg aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga
     4501 agcggttgtt ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga
     4561 gatctatatc tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc
     4621 gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca
     4681 agcagattac ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga
     4741 agtgatgcac tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat
     4801 cggcttcccg gccgcggagt tgttcggtaa attgtcacaa cgccgcgaat atagtcttta
     4861 catgcccttg gccacgcccc tctttaatac gacgggcaat ttgcacttca gaaaatgaag
     4921 agtttgcttt agccataaca aaagtccagt atgctttttc acagcataac tggactgatt
     4981 tcagtttaca actattctgt ctagtttaag actttattgt catagtttag atctattttg
     5041 ttcagtttaa gactttattg tccgcccaca cccgcttacg cagggcatct gcgcatccac
     5101 aggaagagcg acccaagtca atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5161 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5641 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5761 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5821 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5881 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5941 tatgagtaaa cttggtctga cagttgaccc aagtcaaccg gttgtgggcg gacaaaatag
     6001 ttgggaactg ggaggggtgg aaatggagtt tttaaggatt atttagggaa gagtgacaaa
     6061 atagatggga actgggtgta gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag
     6121 tttggaacta gatttcactt atctggttcc aattagatgg gtatacccta ggaaaccaag
     6181 tcaatgtcag tgtttattta aatggttatg atagttattg ctcagcggtg gcagcagcca
     6241 actcagcttc ctttcgggct ttgttagcag ccggatcttc taggctcaag cagtgatcag
     6301 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6361 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6421 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6481 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6541 ctctagtact tctcgacaag cttgtcgaga ctgcaggctc tagattcgaa agcggccgct
     6601 tactatttat ccttttcttt ctgttctttt tctttcttct cacgctcagc tttggcttct
     6661 tcttcctcgt gttttttgat caactgctcc acttcttttt gtttgagaac tctgattact
     6721 gtctttccat tctctcttgt tagtgttgca atttccactt tttcagcaga gagtttacta
     6781 acatccatgg tcttatttag tactttgata gctaaagcaa gtgctgactt caaggtcatt
     6841 tctccttctt tatagtcttg tttcaacatt gacacagctg cagcgctatt atttccaatg
     6901 catgtggcct tccatccccc gtaatttcca ctagggtcac tctgatagag ctgaaagcca
     6961 tagtgcttat cccagccaat gtacagcaat gaaacaccaa agggacgttt tcctccaaat
     7021 tgtgtataag cttgtttgat atcacacagc gctgtaacca actgctcaca aggtattggc
     7081 tcctgatact gtaataaata cctttgagca atgagcctta gttcattagt cagaacatta
     7141 gcatcagaag ttatgcctgc cacactgcaa gccatgtcct cattgagttt ataaattttt
     7201 tcagaaaaaa agacttcatc aagaagcttg tggatgttgc gtctctctgc tgcaagcaaa
     7261 acaccatcat ttgctaaaat tcccaaacag gtgcctgcat gtccaatagc ttccatggca
     7321 tattcaactt ggtataagcg accttctgga gaaaatatag tggtcctgga gtcatatctt
     7381 cgagacatga attccgcgcg cttcggaccg ggatccgcgc ccgatggtgg gacggtatga
     7441 ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac agtaaaatac
     7501 ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta atattccgga
     7561 gtaggtcgat ttaaactaag ctatgtgaac cgtttattta aatggttatg atagttattg
     7621 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcttc
     7681 taggctcaag cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac
     7741 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa att
//
