LOCUS       sequence-432154-        6319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of human 20S CP proteasome subunit alpha 
            type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, 
            Harper JW, Hanna J, Schulman BA
  TITLE     Visualizing chaperone-mediated multistep assembly of the human 20S 
            proteasome.
  JOURNAL   Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
  PUBMED    38600324
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9."
COMMENT     Sequence Label: pACEBac1-PSMA5-PSMA6-PSMA7
FEATURES             Location/Qualifiers
     source          1..6319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    215..349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        504..595
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1569..1703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1859..1950
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    2930..3064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3185..3350
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3540..4128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4029..4048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  4263..4486
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4553..5086)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5275..5280,5281..5297,5298..5303))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5374..5407)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5484..5575
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 catgttcaca aaggaagaac ttgaagaggt tatcaaggac atttaatgag cggccgcttt
       61 cgaatctaga gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag
      121 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      181 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      301 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
      361 tgagcctaga agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      421 ccgctgagca ataactatca taaccattta ataaactgga tactattgca cgtttaaatc
      481 gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
      541 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
      601 ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca
      661 tgtcccgtgg ttccagcgcc ggttttgacc gccacattac cattttttca cccgagggtc
      721 ggctctacca agtagaatat gcttttaagg ctattaacca gggtggcctt acatcagtag
      781 ctgtcagagg gaaagactgt gcggtaattg tcacacagaa gaaagtacct gacaaattat
      841 tggattccag cacagtgact cacttattca agataactga aaacattggt tgtgtgatga
      901 ccggaatgac agctgacagc agatcccagg tacagagggc acgctatgag gcagctaact
      961 ggaaatacaa gtatggctat gagattcctg tggacatgct gtgtaaaaga attgccgata
     1021 tttctcaggt ctacacacag aatgctgaaa tgaggcctct tggttgttgt atgattttaa
     1081 ttggtataga tgaagagcaa ggccctcagg tatataagtg tgatcctgca ggttactact
     1141 gtgggtttaa agccactgca gcgggagtta aacaaactga gtcaaccagc ttccttgaaa
     1201 aaaaagtgaa gaagaaattt gattggacat ttgaacagac agtggaaact gcaattacat
     1261 gcctgtctac tgttctatca attgatttca aaccttcaga aatagaagtt ggagtagtga
     1321 cagttgaaaa tcctaaattc aggattctta cagaagcaga gattgatgct caccttgttg
     1381 ctctagcaga gagagactaa tgagcggccg ctttcgaatc tagagcctgc agtctcgaca
     1441 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     1501 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1561 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1621 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1681 atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac
     1741 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact atcataacca
     1801 tttaaataaa cctaatgatg cctgatgttt aaatcgacct actccggaat attaatagat
     1861 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
     1921 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
     1981 gggcgcggat cccggtccga agcgcgcgga attcatgagc tacgaccgcg ccatcaccgt
     2041 cttctcgccc gacggccacc tcttccaagt ggagtacgcg caggaggccg tcaagaaggg
     2101 ctcgaccgcg gttggtgttc gaggaagaga cattgttgtt cttggtgtgg agaagaagtc
     2161 agtggccaaa ctgcaggatg aaagaacagt gcggaagatc tgtgctttgg atgacaacgt
     2221 ctgcatggcc tttgcaggcc tcaccgccga tgcaaggata gtcatcaaca gggcccgggt
     2281 ggagtgccag agccaccggc tgactgtaga ggacccggtc actgtggagt acatcacccg
     2341 ctacatcgcc agtctgaagc agcgttatac gcagagcaat gggcgcaggc cgtttggcat
     2401 ctctgccctc atcgtgggtt tcgactttga tggcactcct aggctctatc agactgaccc
     2461 ctcgggcaca taccatgcct ggaaggccaa tgccataggc cggggtgcca agtcagtgcg
     2521 cgagttcctg gagaagaact atactgacga agccattgaa acagatgatc tgacgattaa
     2581 gctggtgatc aaggcactcc tggaagtggt tcagtcaggt ggcaaaaaca ttgaacttgc
     2641 tgtcatgagg cgagatcaat ccctcaagat tttaaatcct gaagaaattg agaagtatgt
     2701 tgctgaaatt gaaaaagaaa aagaagaaaa cgaaaagaag aaacaaaaga aagcatcatg
     2761 ataagcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg agaagtacta
     2821 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2881 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3061 gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg
     3121 agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa
     3181 ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
     3241 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
     3301 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca accggttgac
     3361 ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3661 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4201 ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg
     4261 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     4321 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     4381 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     4441 tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca
     4501 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     4561 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     4621 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     4681 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     4741 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     4801 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     4861 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     4921 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     4981 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     5041 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     5101 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     5161 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     5221 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     5281 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     5341 cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat acattatacg
     5401 aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc
     5461 gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
     5521 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
     5581 ggattattca taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca
     5641 tgtttcttac ccggtctgag tacgacaggg gcgtgaatac tttttctccc gaaggaagat
     5701 tatttcaagt ggaatatgcc attgaggcta tcaagcttgg ttctacagcc attgggatcc
     5761 agacatcaga gggtgtgtgc ctagctgtgg agaagagaat tacttcccca ctgatggagc
     5821 ccagcagcat tgagaaaatt gtagagattg atgctcacat aggttgtgcc atgagtgggc
     5881 taattgctga tgctaagacc ttaattgata aagccagagt ggagacacag aaccactggt
     5941 tcacctacaa tgagacaatg acagtggaga gtgtgaccca agctgtgtcc aatctggctt
     6001 tgcagtttgg agaagaagat gcagatccag gtgccatgtc tcgtcccttt ggagtagcat
     6061 tattatttgg aggagttgat gagaaaggac cccagctgtt tcatatggac ccatctggga
     6121 cctttgtaca gtgtgatgct cgagcaattg gctctgcttc agagggtgcc cagagctcct
     6181 tgcaagaagt ttaccacaag tctatgactt tgaaagaagc catcaagtct tcactcatca
     6241 tcctcaaaca agtaatggag gagaagctga atgcaacaaa cattgagcta gccacagtgc
     6301 agcctggcca gaatttcca
//
