LOCUS       sequence-432156-        7571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect cell expression of human 20S CP proteasome subunit beta 
            type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta 
            type-4 (PSMB4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, 
            Harper JW, Hanna J, Schulman BA
  TITLE     Visualizing chaperone-mediated multistep assembly of the human 20S 
            proteasome.
  JOURNAL   Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
  PUBMED    38600324
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9."
COMMENT     Sequence Label: pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4
FEATURES             Location/Qualifiers
     source          1..7571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    51..185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1036..1127)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1283..1417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2331..2422)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    2578..2712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3671..3762)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    3839..3872
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(3943..3948,3949..3965,3966..3971)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4160..4693
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4760..4983
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(5118..5706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5198..5217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5896..6061
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    6213..6347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7375..7466)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca gatccagaca
       61 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      241 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagtac
      301 ttctcgacaa gcttgtcgag actgcaggct ctagattcga aagcggccgc tcattagtcc
      361 attcgggcct tcagtgtcct ggtggtgatt ttgtccttct cgatgatgtg gacaatgact
      421 cccatgcctg acactgcatc ccggtccaca gcattcagca tggcttggga gatggtttca
      481 aacaggtgat ccggatccat gttgggctcc cagagggact cacacattcc gtacatttgt
      541 tcggcgcagg tgccactgac cacaaagtca tcagtcacca tggggcagcc gatgaggtct
      601 agagagcaaa tgaagggctt aaaggtcttc gggtccaacc cggcaatgac tggctcagtg
      661 tagtaagggc caaaccgttt ctcatacaag aggttggcca ccatgctcat gagggtataa
      721 ggtttgatct gccgaccttc cttcaactca tacaggttca gccggaactt gaggcgctgg
      781 gcaactgtct ggacgtcagt ggcgagcccg gccagaccga tgtacagccg gtcacccatg
      841 ggaaagatct tctggaagtc cgtggtcacc atctgggcct ggatcccgaa gcgcctgtct
      901 gcagcgatgg ccacacagtt cttccccttc atggccatga cggcccctcc gttataggac
      961 ataatagaca tgaattccgc gcgcttcgga ccgggatccg cgcccgatgg tgggacggta
     1021 tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa aacagtaaaa
     1081 tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatcta ttaatattcc
     1141 ggagtaggtc gatttaaaca tcaggcatca ttaggtttat ttaaatggtt atgatagtta
     1201 ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc
     1261 ttctaggctc aagcagtgat cagatccaga catgataaga tacattgatg agtttggaca
     1321 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1381 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1441 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1501 atgtggtatg gctgattatg atcctctagt acttctcgac aagcttgtcg agactgcagg
     1561 ctctagattc gaaagcggcc gctcattagg agccctgttt ggggaaggaa atgttatcca
     1621 ggtcatggat gccatttttg tcaatgattc gaacactgaa ggttggcaga ttcaggatga
     1681 agcgtttctg gagctcctcc agacatttcc taaggagttc cactgccctc tcacgtgaga
     1741 tagtcggtgt gtagtatcgg tcgaggatac tgagagtcag gaaggcacca tagccgtgcg
     1801 ctgcaaaagg ggccttggcc aaggctgcca ggtagtccat gtaatacagc gctggccctt
     1861 catgctcatc atagccagcc aggaggaggt tcacatgata tggggtccga ctccgaagac
     1921 agtcagccag gtttcggcgt gtgaagttag ctgctgccgt gggagacaat tcatatccat
     1981 ttcgcatctt ataaagttgc acgtttttct gaatatattc tgcaaactgt acagtgtctc
     2041 cagcctctcc aacacacagg agtaatatct tttcactcat cttaaacatc ttgtcatgat
     2101 cgtccttcat ctggacaata ttgctggcgg ccacccggtc ggaggcgaca agaacatagt
     2161 cggggccttg gataccgatg aggtactcca tgaattccgc gcgcttcgga ccgggatcta
     2221 gattcgaaag cggccgcgac aagtgagctc gtcgacgtag gcctttgaat tccgcgcgct
     2281 tcggaccggg atccgcgccc gatggtggga cggtatgaat aatccggaat atttataggt
     2341 ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc gagatggtta
     2401 tcattttaat tatctccatg atctattaat attccggagt aggtcgattt aaacgtgcaa
     2461 tagtatccag tttatttaaa tggttatgat agttattgct cagcggtggc agcagccaac
     2521 tcagcttcct ttcgggcttt gttagcagcc ggatcttcta ggctcaagca gtgatcagat
     2581 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2641 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2701 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     2761 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     2821 ctagtacttc tcgacaagct tgtcgagact gcaggctcta gattcgaaag cggccgctta
     2881 tcagtccttc cttaaggaaa cagtttcctc cctgatgccc tctttggtca ctatgcagat
     2941 ccggagtgcg tccccagtgt acacatctct ctcagccgca gaaatgaaga catctttcac
     3001 cagccgcatc gctctgtcca aggacagcgg aacatgctcc acattctgca tgttcttaaa
     3061 accaacctgg ttgtcaagca ggggctgtag catggcactt gctgagcctc cagccttgaa
     3121 ggagtctctc tggtaagacc ctactggatc aaagctgtat acagccccct ttccttcttc
     3181 atcaagtcca ccgatgatgt tgtaaacata gtatggaaag aagcgccttg aatacaggat
     3241 tgtagacagc attgcagcaa ttgcccccgt agtcatggcc ttattattgg aatgcttata
     3301 catctttagt cttgcttcaa taatctttgt cagcgtaaga cagtctccat gaaaaccgct
     3361 gcatccaatg actgttttgt ctgttaattt gtaacatttg gggctatccc gcgtatgaat
     3421 tgaaaaccct tcactcaatc gagtatcaga agcaacaatt gcaaaatctt ctccagcaat
     3481 tgccagtata gtacctccgt tgaaaacgta gggcgaaaat cgcagctgca aagggcccgc
     3541 ggctctgtgc ggttccatcc ccaagtctct gccaggagcc gaatacatgg ctgtagagga
     3601 caacatgaat tccgcgcgct tcggaccggg atccgcgccc gatggtggga cggtatgaat
     3661 aatccggaat atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt
     3721 atttatttgc gagatggtta tcattttaat tatctccatg atctattaat attccggagt
     3781 aggtcgcgaa tcgatactag tgtttaaact cgctacctta ggaccgttat agttacagat
     3841 aacttcgtat aatgtatgct atacgaagtt atggcgactt cgctgctgcc caaggttgcc
     3901 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agttgacata agcctgttcg
     3961 gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     4021 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     4081 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     4141 atgttatgga gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg
     4201 ccctaaaaca aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc
     4261 tgaccaagtc aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt
     4321 agccacctac tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa
     4381 gacattcatc gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta
     4441 cgttctgccc aagtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc
     4501 cggcgagcac cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc
     4561 caacgcgctt ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt
     4621 ggctctctat acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag
     4681 taccgccacc taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg
     4741 taaattgtca caacgccgcg aatatagtct ttacatgccc ttggccacgc ccctctttaa
     4801 tacgacgggc aatttgcact tcagaaaatg aagagtttgc tttagccata acaaaagtcc
     4861 agtatgcttt ttcacagcat aactggactg atttcagttt acaactattc tgtctagttt
     4921 aagactttat tgtcatagtt tagatctatt ttgttcagtt taagacttta ttgtccgccc
     4981 acacccgctt acgcagggca tctgcgcatc cacaggaaga gcgacccaag tcaatcaggg
     5041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5521 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5761 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5821 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttga
     5881 cccaagtcaa ccggttgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     5941 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     6001 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     6061 tccaattaga tgggtatacc ctaggaaacc aagtcaatgt cagtgtttat ttaaatggtt
     6121 atgatagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
     6181 cagccggatc ttctaggctc aagcagtgat cagatccaga catgataaga tacattgatg
     6241 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6301 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6361 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     6421 acctctacaa atgtggtatg gctgattatg atcctctagt acttctcgac aagcttgtcg
     6481 agactgcagg ctctagattc gaaagcggcc gcttatcatt caaagccact gatcatgtgg
     6541 gcaatatccc agttggtctc tgtagacaat ggtccctcta tttcaacacc tttttcggtg
     6601 acagtggcgg tttgaaaccg gttgtaagaa cgggcatctc ggtagtacag cactcgcatg
     6661 cagcgttcta ctaagtcgcg ggcctcggtc tggcttagca ctggctgctt ctccagaact
     6721 tctcgcagca gaggctgagc caagtatgca ccataaccag tggccagcga aggggcttca
     6781 taggctacac caagcatgtc cacataaccg aggaagctct ctccatcagc atagcctccg
     6841 atgaccatgg tgttccacaa agggttcatc ttcgagcgcc ggctgtacat ggccctggtc
     6901 agccatgaat gaatagctct aggactatag ctgtgtccat ctcccagaag ctcctcatca
     6961 atcaccatct ggccgagaac ttgcttcaaa tactggaaat cagcgtagtc gccagaggca
     7021 cccagcatgg tactgttgtt gactcgcata atgcgagaga tgttgcggaa acgagccaag
     7081 gagccgtagg atcccagcat gtctgcggca atcaccactc cgccctcgaa cttaacgccg
     7141 aggactgagg tcccggtcac catggggttc tgggtccgcg tgattggacc tctgtaaagt
     7201 gcagacgccg gatccatgaa ggaatcggga gtggacggaa tgcggtaaaa ctgtcctggg
     7261 gccggacccc ccgcccaaag tccggaccgc gaccccaaaa acgcttccat gaattccgcg
     7321 cgcttcggac cgggatccgc gcccgatggt gggacggtat gaataatccg gaatatttat
     7381 aggttttttt attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg
     7441 gttatcattt taattatctc catgatctat taatattccg gagtaggtcg atttaaacta
     7501 agctatgtga accgtttatt taaatggtta tgatagttat tgctcagcgg tggcagcagc
     7561 caactcagct t
//
