LOCUS       sequence-432157-       13424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLRP3 tagged with mNeonGreen in mammalian cells 
            (lentiviral); driven by the weak UBC promoter with puro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
  TITLE     Spatiotemporal proteomic profiling of cellular responses to NLRP3 
            agonists
  JOURNAL   bioRxiv 2024.04.19.590338
REFERENCE   2  (bases 1 to 13424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.19.590338"
COMMENT     Sequence Label: pLenti UBC hNLRP3-mNG
FEATURES             Location/Qualifiers
     source          1..13424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1069..1267
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2134..2367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2552..2596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2894..3011
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3103..4314
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4151..4170
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4362..4386
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7530..8237
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      7782..7791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8239..8263)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8310..8399
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        8441..8940
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8476..8499)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8873..8892
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8961..9560
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8961..8980)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9457..9477
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(9591..9624)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9680..10268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9733..9753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10151..10162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10271..10287)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10272..10288)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10477..10710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10782..10903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10819..10838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10873..10892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10943..11078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11098..11117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11099..11117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(11140..11498)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11180..11201)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11392..11411
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11525..11629
                     /gene="bla"
                     /label=AmpR promoter
     promoter        11631..11988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11631..11822
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11654..11674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11839..11974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12023..12817
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             12023..12817
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12077..12096)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12687..12706
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(13005..13024)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13049..13096
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     2041 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     2101 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     2161 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     2221 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     2281 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     2341 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2401 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2461 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2521 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2581 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2641 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2701 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2761 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2821 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2881 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2941 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3001 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc
     3061 cgctctagcc ctagatttaa cccaacagaa ggctcgacgc gtggcctccg cgccgggttt
     3121 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     3181 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     3241 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     3301 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     3361 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     3421 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     3481 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     3541 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     3601 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     3661 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     3721 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     3781 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     3841 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     3901 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     3961 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     4021 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     4081 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     4141 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     4201 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     4261 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgctaga
     4321 ctgccggatc gaattgtcga cgaattcgga tctggccaat cacaagtttg tacaaaaaag
     4381 caggctccac catgaagatg gcaagcaccc gctgcaagct ggccaggtac ctggaggacc
     4441 tggaggatgt ggacttgaag aaatttaaga tgcacttaga ggactatcct ccccagaagg
     4501 gctgcatccc cctcccgagg ggtcagacag agaaggcaga ccatgtggat ctagccacgc
     4561 taatgatcga cttcaatggg gaggagaagg cgtgggccat ggccgtgtgg atcttcgctg
     4621 cgatcaacag gagagacctt tatgagaaag caaaaagaga tgagccgaag tggggttcag
     4681 ataatgcacg tgtttcgaat cccactgtga tatgccagga agacagcatt gaagaggagt
     4741 ggatgggttt actggagtac ctttcgagaa tctctatttg taaaatgaag aaagattacc
     4801 gtaagaagta cagaaagtac gtgagaagca gattccagtg cattgaagac aggaatgccc
     4861 gtctgggtga gagtgtgagc ctcaacaaac gctacacacg actgcgtctc atcaaggagc
     4921 accggagcca gcaggagagg gagcaggagc ttctggccat cggcaagacc aagacgtgtg
     4981 agagccccgt gagtcccatt aagatggagt tgctgtttga ccccgatgat gagcattctg
     5041 agcctgtgca caccgtggtg ttccaggggg cggcagggat tgggaaaaca atcctggcca
     5101 ggaagatgat gttggactgg gcgtcgggga cactctacca agacaggttt gactatctgt
     5161 tctatatcca ctgtcgggag gtgagccttg tgacacagag gagcctgggg gacctgatca
     5221 tgagctgctg ccccgaccca aacccaccca tccacaagat cgtgagaaaa ccctccagaa
     5281 tcctcttcct catggacggc ttcgatgagc tgcaaggtgc ctttgacgag cacataggac
     5341 cgctctgcac tgactggcag aaggccgagc ggggagacat tctcctgagc agcctcatca
     5401 gaaagaagct gcttcccgag gcctctctgc tcatcaccac gagacctgtg gccctggaga
     5461 aactgcagca cttgctggac catcctcggc atgtggagat cctgggtttc tccgaggcca
     5521 aaaggaaaga gtacttcttc aagtacttct ctgatgaggc ccaagccagg gcagccttca
     5581 gtctgattca ggagaacgag gtcctcttca ccatgtgctt catccccctg gtctgctgga
     5641 tcgtgtgcac tggactgaaa cagcagatgg agagtggcaa gagccttgcc cagacatcca
     5701 agaccaccac cgcggtgtac gtcttcttcc tttccagttt gctgcagccc cggggaggga
     5761 gccaggagca cggcctctgc gcccacctct gggggctctg ctctttggct gcagatggaa
     5821 tctggaacca gaaaatcctg tttgaggagt ccgacctcag gaatcatgga ctgcagaagg
     5881 cggatgtgtc tgctttcctg aggatgaacc tgttccaaaa ggaagtggac tgcgagaagt
     5941 tctacagctt catccacatg actttccagg agttctttgc cgccatgtac tacctgctgg
     6001 aagaggaaaa ggaaggaagg acgaacgttc cagggagtcg tttgaagctt cccagccgag
     6061 acgtgacagt ccttctggaa aactatggca aattcgaaaa ggggtatttg atttttgttg
     6121 tacgtttcct ctttggcctg gtaaaccagg agaggacctc ctacttggag aagaaattaa
     6181 gttgcaagat ctctcagcaa atcaggctgg agctgctgaa atggattgaa gtgaaagcca
     6241 aagctaaaaa gctgcagatc cagcccagcc agctggaatt gttctactgt ttgtacgaga
     6301 tgcaggagga ggacttcgtg caaagggcca tggactattt ccccaagatt gagatcaatc
     6361 tctccaccag aatggaccac atggtttctt ccttttgcat tgagaactgt catcgggtgg
     6421 agtcactgtc cctggggttt ctccataaca tgcccaagga ggaagaggag gaggaaaagg
     6481 aaggccgaca ccttgatatg gtgcagtgtg tcctcccaag ctcctctcat gctgcctgtt
     6541 ctcatggatt ggtgaacagc cacctcactt ccagtttttg ccggggcctc ttttcagttc
     6601 tgagcaccag ccagagtcta actgaattgg acctcagtga caattctctg ggggacccag
     6661 ggatgagagt gttgtgtgaa acgctccagc atcctggctg taacattcgg agattgtggt
     6721 tggggcgctg tggcctctcg catgagtgct gcttcgacat ctccttggtc ctcagcagca
     6781 accagaagct ggtggagctg gacctgagtg acaacgccct cggtgacttc ggaatcagac
     6841 ttctgtgtgt gggactgaag cacctgttgt gcaatctgaa gaagctctgg ttggtcagct
     6901 gctgcctcac atcagcatgt tgtcaggatc ttgcatcagt attgagcacc agccattccc
     6961 tgaccagact ctatgtgggg gagaatgcct tgggagactc aggagtcgca attttatgtg
     7021 aaaaagccaa gaatccacag tgtaacctgc agaaactggg gttggtgaat tctggcctta
     7081 cgtcagtctg ttgttcagct ttgtcctcgg tactcagcac taatcagaat ctcacgcacc
     7141 tttacctgcg aggcaacact ctcggagaca aggggatcaa actactctgt gagggactct
     7201 tgcaccccga ctgcaagctt caggtgttgg aattagacaa ctgcaacctc acgtcacact
     7261 gctgctggga tctttccaca cttctgacct ccagccagag cctgcgaaag ctgagcctgg
     7321 gcaacaatga cctgggcgac ctgggggtca tgatgttctg tgaagtgctg aaacagcaga
     7381 gctgcctcct gcagaacctg gggttgtctg aaatgtattt caattatgag acaaaaagtg
     7441 cgttagaaac acttcaagaa gaaaagcctg agctgaccgt cgtctttgag ccttcttggt
     7501 tgggcggatc cgggggaagc ggcggatccg tgagcaaggg cgaggaggat aacatggcct
     7561 ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg gactttgaca
     7621 tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac ctgaagtcca
     7681 ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg tatggcttcc
     7741 atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg gtagatggct
     7801 ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt actgttaact
     7861 accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag gggactggtt
     7921 tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg tgcaggtcga
     7981 agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt tacaccactg
     8041 gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc aagccaatgg
     8101 cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag ctcaagcact
     8161 ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg atgggcatgg
     8221 acgagctgta caagtaggac ccagctttct tgtacaaagt ggtgggtgga ggcggttcag
     8281 gtggcggagg aagcggaggc ggtggaagtt acccctacga cgtgcccgac tacgccggct
     8341 atccgtatga tgtcccggac tatgcaggat cctatccata cgacgttcca gattacgctt
     8401 agttaattaa ggccggccct cgaggttaac gaattctacc gggtagggga ggcgcttttc
     8461 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     8521 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     8581 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     8641 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     8701 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     8761 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     8821 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     8881 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     8941 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9001 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     9061 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     9121 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     9181 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     9241 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     9301 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     9361 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     9421 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     9481 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     9541 cccgcaagcc cggtgcctga tgtacaagta ggaattcgtc gagggaccta ataacttcgt
     9601 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
     9661 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9721 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9781 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9841 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9901 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9961 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10021 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    10081 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    10141 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    10201 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    10261 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
    10321 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
    10381 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
    10441 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    10501 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
    10561 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10621 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    10681 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
    10741 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
    10801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
    10921 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    10981 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11041 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
    11101 tatagtgagt cgtattagca tgtgagcaaa aggccagcat aaatcaaaag aatagaccga
    11161 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    11221 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    11281 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    11341 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    11401 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    11461 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
    11521 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    11581 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
    11641 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    11701 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    11761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    11821 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    11881 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    11941 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
    12001 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
    12061 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    12121 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    12181 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
    12241 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    12301 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    12361 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    12421 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    12481 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    12541 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    12601 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    12661 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    12721 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    12781 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    12841 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    12901 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    12961 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
    13021 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
    13081 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
    13141 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
    13201 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
    13261 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    13321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    13381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
